About   Help   FAQ
Gene Expression Data
Assay Details
Assay
Reference: J:208435 Yi SH, et al., Foxa2 acts as a co-activator potentiating expression of the Nurr1-induced DA phenotype via epigenetic regulation. Development. 2014 Feb;141(4):761-72
Assay type: Immunohistochemistry
MGI Accession ID: MGI:7468756
Gene symbol: Foxa2
Gene name: forkhead box A2
Antibody: anti-Foxa2 (M-20; sc-6554)
Detection system: Secondary antibody coupled to Alexa Fluor 488
Results
Specimen 1A/C: embryonic day 9.0 (more )
Note: Double labeled: green - Foxa2; red - Nr4a2 (assay MGI:7468764).
Structure Level Pattern Image Note
TS14: future midbrain Present Regionally restricted 1A , 1C Expressed in the ventral region.
Specimen 1A inset: embryonic day 9.0 (more )
Note: Double labeled: green - Foxa2; red - Pcna (assay MGI:7468812).
Structure Level Pattern Image Note
TS14: future midbrain Present Regionally restricted 1A inset Expressed in the ventral region, coexpressed with Pcna.
Specimen 1D/F: embryonic day 12.0 (more )
Note: Double labeled: green - Foxa2; red - Nr4a2 (assay MGI:7468764).
Structure Level Pattern Image Note
TS20: midbrain mantle layer Present 1D , 1F Expression was broader than that of Nr4a2, to the lateral portions.
TS20: midbrain ventricular layer Present 1D , 1F Weaker expression than in the mantle zone.
Specimen 1D inset: embryonic day 12.0 (more )
Note: Double labeled: green - Foxa2; red - Pcna (assay MGI:7468812).
Structure Level Pattern Image Note
TS20: midbrain Present 1D inset Some of the positive cells coexpressed Pcna.
Specimen 1G/I: embryonic day 15.0 (more )
Note: Double labeled: green - Foxa2; red - Nr4a2 (assay MGI:7468764).
Structure Level Pattern Image Note
TS23: midbrain Present Regionally restricted 1G , 1I Expression domain became narrower and was restricted to the domain of Nr4a2+ cells.
Specimen 1M: embryonic day 12.0 (more )
Note: Triple labeled: green - Foxa2; blue - Th (assay MGI:7468767); red - Nr4a2 (assay MGI:7468764).
Structure Level Pattern Image Note
TS20: midbrain mantle layer Present 1M
TS20: midbrain ventricular layer Present 1M
Specimen 5A: embryonic day 12.0 (more )
Note: Double labeled: green - Foxa2; red - Nr4a2 (assay MGI:7468764).
Structure Cell Type Level Pattern Image Note
TS20: midbrain mantle layer midbrain dopaminergic neuron Present 5A Foxa2 , 5A merge A granular distribution within the nucleus was shown, coexpressed with Nr4a2 (taken from Th+ cells in Fig 1M).
Specimen S1G: embryonic day 12.5 (more )
Note: Double labeled: green - Foxa2; red - Nr4a2 (assay MGI:7468764).
Structure Level Pattern Image Note
TS20: midbrain mantle layer Present S1G right , S1G left
TS20: midbrain ventricular layer Present S1G right , S1G left Weaker expression than in the mantle zone.
Specimen not shown: postnatal adult (more )
Structure Cell Type Level Pattern Image Note
TS28: midbrain midbrain dopaminergic neuron Present Exclusively colocalized in Th+ dopaminergic neurons.
TS28: hippocampus hippocampal CA1-3 neuron Absent
Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
Citing These Resources
Funding Information
Warranty Disclaimer, Privacy Notice, Licensing, & Copyright
Send questions and comments to User Support. 		last database update
05/07/2024
MGI 6.23 		The Jackson Laboratory