Gene Expression Data
Assay Details
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Assay
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Reference:
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J:249296
Spiller CM, et al., Retinoblastoma 1 protein modulates XY germ cell entry into G1/G0 arrest during fetal development in mice. Biol Reprod. 2010 Feb;82(2):433-43
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Assay type:
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Immunohistochemistry
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MGI Accession ID:
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MGI:7467392
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Gene symbol:
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Ddx4
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Gene name:
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DEAD box helicase 4
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Antibody:
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anti-DDX4
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Detection system:
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Secondary antibody coupled to Alexa Fluor
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Results
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Specimen
3A:
embryonic day 14.5; Rb1tm1.2Gfk/Rb1 +
(more  )
Specimen
3A:
(close  )
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Genetic Background:
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involves: 129T2/SvEms * BALB/cJ * C57BL/6
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Mutant Allele(s):
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Rb1tm1.2Gfk/Rb1+
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Age:
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embryonic day 14.5
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Sex:
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Male
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Paraffin
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Specimen
3B:
(close )
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Genetic Background:
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involves: 129T2/SvEms * BALB/cJ * C57BL/6
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Mutant Allele(s):
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Rb1tm1.2Gfk/Rb1tm1.2Gfk
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Age:
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embryonic day 14.5
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Sex:
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Male
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Paraffin
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| Structure |
Level |
Pattern |
Image |
Note |
| TS22: germ cell of testis |
Present |
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3B
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There is a non-statistically significant increase of 19% in the number of germ cells in the mutants.
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Specimen
3E:
embryonic day 14.5; Rb1tm1.2Gfk/Rb1 +
(more )
Note: Double labeled: green - Ddx4; red - Mki67 (assay MGI:7467396).
Specimen
3E:
(close )
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Genetic Background:
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involves: 129T2/SvEms * BALB/cJ * C57BL/6
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Mutant Allele(s):
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Rb1tm1.2Gfk/Rb1+
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Age:
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embryonic day 14.5
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Sex:
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Male
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Paraffin
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Note:
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Double labeled: green - Ddx4; red - Mki67 (assay MGI:7467396).
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| Structure |
Level |
Pattern |
Image |
Note |
| TS22: germ cell of testis |
Present |
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3E
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In wild type the cells have stopped proliferating and entered cell cycle arrest.
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Specimen
3F:
(close )
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Genetic Background:
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involves: 129T2/SvEms * BALB/cJ * C57BL/6
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Mutant Allele(s):
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Rb1tm1.2Gfk/Rb1tm1.2Gfk
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Age:
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embryonic day 14.5
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Sex:
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Male
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Paraffin
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Note:
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Double labeled: green - Ddx4; red - Mki67 (assay MGI:7467396).
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| Structure |
Level |
Pattern |
Image |
Note |
| TS22: germ cell of testis |
Present |
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3F
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In the mutant 4% (a significant number) of the germ cells are still proliferating.
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Specimen
3G:
embryonic day 14.5; Rb1tm1.2Gfk/Rb1 +
(more )
Note: Also stained for BrdU (red).
Specimen
3G:
(close )
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Genetic Background:
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involves: 129T2/SvEms * BALB/cJ * C57BL/6
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Mutant Allele(s):
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Rb1tm1.2Gfk/Rb1+
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Age:
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embryonic day 14.5
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Sex:
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Male
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Paraffin
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Note:
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Also stained for BrdU (red).
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| Structure |
Level |
Pattern |
Image |
Note |
| TS22: germ cell of testis |
Present |
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3G
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In wild type the cells have stopped proliferating and entered cell cycle arrest.
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Specimen
3H:
(close )
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Genetic Background:
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involves: 129T2/SvEms * BALB/cJ * C57BL/6
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Mutant Allele(s):
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Rb1tm1.2Gfk/Rb1tm1.2Gfk
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Age:
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embryonic day 14.5
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Sex:
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Male
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Paraffin
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Note:
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Also stained for BrdU (red).
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| Structure |
Level |
Pattern |
Image |
Note |
| TS22: germ cell of testis |
Present |
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3H
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In the mutant 4% (a significant number) of the germ cells are still proliferating.
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Specimen
6A:
embryonic day 14.5; Rb1tm1.2Gfk/Rb1 +
(more )
Note: Also stained for a cell death marker (red).
Specimen
6A:
(close )
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Genetic Background:
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involves: 129T2/SvEms * BALB/cJ * C57BL/6
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Mutant Allele(s):
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Rb1tm1.2Gfk/Rb1+
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Age:
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embryonic day 14.5
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Sex:
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Male
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Paraffin
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Note:
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Also stained for a cell death marker (red).
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| Structure |
Level |
Pattern |
Image |
Note |
| TS22: germ cell of testis |
Present |
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6A
,
6C
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Observed cell death is in the somatic cell population.
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Specimen
6B:
embryonic day 14.5; Rb1tm1.2Gfk/ Rb1tm1.2Gfk
(more )
Note: Also stained for a cell death marker (red).
Specimen
6B:
(close )
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Genetic Background:
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involves: 129T2/SvEms * BALB/cJ * C57BL/6
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Mutant Allele(s):
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Rb1tm1.2Gfk/Rb1tm1.2Gfk
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Age:
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embryonic day 14.5
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Sex:
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Male
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Paraffin
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Note:
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Also stained for a cell death marker (red).
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| Structure |
Level |
Pattern |
Image |
Note |
| TS22: germ cell of testis |
Present |
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6D
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6B
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Observed cell death is in the somatic cell population.
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Analysis Tools