Gene Expression Data
Assay Details
Assay
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Reference:
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J:334332
Pilaz LJ, et al., Subcellular mRNA localization and local translation of Arhgap11a in radial glial progenitors regulates cortical development. Neuron. 2023 Mar 15;111(6):839-856.e5
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Assay type:
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RNA in situ
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MGI Accession ID:
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MGI:7461498
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Gene symbol:
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Arhgap11a
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Gene name:
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Rho GTPase activating protein 11A
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Probe:
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Arhgap11a smFISH probe
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Visualized with:
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Fluorescence
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Assay notes:
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Two Arhgap11a antibodies were described, but the one used in this assay was not detailed. Anti-Arhgap11a (ab113261) MGI:7461491 may have been used in this assay.
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Results
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Specimen
1K Arhgap11a smFISH:
embryonic day 16.5
(more )
Specimen
1K Arhgap11a smFISH:
(close )
Genetic Background:
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C57BL/6J
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Age:
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embryonic day 16.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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Structure |
Cell Type |
Level |
Pattern |
Image |
Note |
TS24: cerebral cortex marginal layer |
forebrain radial glial cell |
Present |
Regionally restricted |
1K (right)
,
1K (left)
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Expression in radial glial cell endfeet at pia.
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Specimen
1M Arhgap11a smFISH:
embryonic day 16.5
(more )
Note: Double labeled: red - Arhgap11a RNA; green - Arhgap11a protein (assay MGI:7461494).
Specimen
1M Arhgap11a smFISH:
(close )
Genetic Background:
|
C57BL/6J
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Age:
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embryonic day 16.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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Note:
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Double labeled: red - Arhgap11a RNA; green - Arhgap11a protein (assay MGI:7461494).
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Structure |
Cell Type |
Level |
Pattern |
Image |
Note |
TS24: cerebral cortex marginal layer |
forebrain radial glial cell |
Present |
Regionally restricted |
1M
,
1M inset
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Expression in radial glial cell endfeet at pia colocalizing with Arhgap11a protein expression.
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