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Gene Expression Data
Assay Details
Assay
Reference: J:331484 Yamada S, et al., Identification and expression profile of novel STAND gene Nwd2 in the mouse central nervous system. Gene Expr Patterns. 2022 Dec;46:119284
Assay type: RNA in situ
MGI Accession ID: MGI:7450905
Gene symbol: Nwd2
Gene name: NACHT and WD repeat domain containing 2
Probe: Nwd2 cDNA2
Probe preparation: Antisense labelled with alkaline phosphatase RNA
Visualized with: Fluorescence
Results
Specimen 5B: embryonic day 14.5 (more )
Note: Double labeled: red - Nwd2; green - Elavl3 (assay MGI:7450821) and Elavl4 (assay MGI:7450904).
Structure Cell Type Level Pattern Image Note
TS22: habenula neuron Present 5C , 5E , 5B , 5F , 5F' Co-labeling was detected in all Elavl3/4-immunopositive cells in the embryonic habenula. Almost all Nwd2-positive cells were positive for Elavl3/4.

Specimen 5H: postnatal day 14 (more )
Note: Double labeled: red - Nwd2; green - Elavl3 (assay MGI:7450821) and Elavl4 (assay MGI:7450904).
Structure Cell Type Level Pattern Image Note
TS28: medial habenular nucleus neuron Present 5H , 5L , 5K , 5L' , 5I Expression was detected in the medial habenula. Co-labeling with Elavl3/4 was detected.
TS28: lateral habenular nucleus neuron Present Scattered 5L , 5H , 5L'' , 5I , 5K Expression was observed in a few scattered neurons. No Nwd2-positive;Elavl3/4-negative cells were seen in lateral habenula.

Specimen 5N: postnatal adult (more )
Note: Double labeled: red - Nwd2; green - Elavl3 (assay MGI:7450821) and Elavl4 (assay MGI:7450904).
Structure Cell Type Level Pattern Image Note
TS28: medial habenular nucleus neuron Present 5N , 5O , 5R' , 5Q , 5R Expression was detected in the medial habenula. Co-labeling with Elavl3/4 was detected.
TS28: lateral habenular nucleus neuron Present Scattered 5R'' , 5N , 5Q , 5O , 5R Expression was observed in a few scattered neurons. No Nwd2-positive;Elavl3/4-negative cells were seen in lateral habenula.

Specimen 6B: embryonic day 14.5 (more )
Note: Double labeled: red - Nwd2; green - Rbfox3 (assay MGI:7451106).
Structure Cell Type Level Pattern Image Note
TS22: habenula neuron Present 6C' , 6B , 6C Nwd2 was expressed in the habenula earlier than Rbfox3 labeling was detected.

Specimen 6E: postnatal day 14 (more )
Note: Double labeled: red - Nwd2; green - Rbfox3 (assay MGI:7451106).
Structure Cell Type Level Pattern Image Note
TS28: medial habenular nucleus neuron Present 6F' , 6E , 6F All Nwd2-positive neurons were also positive for Rbfox3.
TS28: lateral habenular nucleus neuron Present 6E , 6F'' , 6F All Nwd2-positive neurons were also positive for Rbfox3.

Specimen 6H: postnatal adult (more )
Note: Double labeled: red - Nwd2; green - Rbfox3 (assay MGI:7451106).
Structure Cell Type Level Pattern Image Note
TS28: medial habenular nucleus neuron Present 6I' , 6I , 6H All Nwd2-positive neurons were also positive for Rbfox3.
TS28: lateral habenular nucleus neuron Present 6I , 6H , 6I'' All Nwd2-positive neurons were also positive for Rbfox3.

Specimen 6K: embryonic day 14.5 (more )
Note: Double labeled: red - Nwd2; green - Chat (assay MGI:7451107).
Structure Cell Type Level Pattern Image Note
TS22: habenula neuron Present 6K , 6L' , 6L Nwd2 was expressed in the habenula earlier than Chat labeling was detected.

Specimen 6N: postnatal day 14 (more )
Note: Double labeled: red - Nwd2; green - Chat (assay MGI:7451107).
Structure Cell Type Level Pattern Image Note
TS28: medial habenular nucleus cholinergic neuron Present Regionally restricted 6N , 6O' , 6O Expression in the ventral medial habenula overlapped with Chat expression in cholinergic neurons.
TS28: medial habenular nucleus neuron Present Regionally restricted 6O , 6O'' , 6N Expression of Nwd2 in dorsal medial habenula was devoid of Chat expression.

Specimen 6Q: postnatal adult (more )
Note: Double labeled: red - Nwd2; green - Chat (assay MGI:7451107).
Structure Cell Type Level Pattern Image Note
TS28: medial habenular nucleus cholinergic neuron Present Regionally restricted 6Q , 6R' , 6R Expression in the ventral medial habenula overlapped with Chat expression in cholinergic neurons.
TS28: medial habenular nucleus neuron Present Regionally restricted 6R , 6Q , 6R'' Expression of Nwd2 in dorsal medial habenula was devoid of Chat expression.


Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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last database update
10/22/2024
MGI 6.24
The Jackson Laboratory