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Gene Expression Data
Assay Details
Assay
Reference: J:236440 You L, et al., The chromatin regulator Brpf1 regulates embryo development and cell proliferation. J Biol Chem. 2015 May 1;290(18):11349-64
Assay type: Immunohistochemistry
MGI Accession ID: MGI:7447077
Gene symbol: Casp3
Gene name: caspase 3
Antibody: anti-cleaved Caspase-3 (Asp175; #9661)
Detection system: Secondary antibody coupled to Alexa Fluor 568
Assay notes: Fixation was with 12% glacial acetic acid.
Results
Specimen 6A Control: embryonic day 8.5; Brpf1tm1c(EUCOMM)Wtsi/Brpf1+ (more )
Structure Level Pattern Image Note
TS13: neural ectoderm Absent 6A Control/DAPI , 6A Control
Specimen 6A Brdf1-null: embryonic day 8.5; Brpf1tm1d(EUCOMM)Wtsi/Brpf1tm1d(EUCOMM)Wtsi (more )
Structure Level Pattern Image Note
TS13: neural ectoderm Absent 6A Brpf1-null/DAPI , 6A Brpf1-null No evidence of apoptosis at E8.5.
Specimen 6B Control: embryonic day 9.5; Brpf1tm1c(EUCOMM)Wtsi/Brpf1+ (more )
Structure Level Pattern Image Note
TS15: future midbrain Absent 6B Control/DAPI , 6B Control
Specimen 6B Brdf1-null: embryonic day 9.5; Brpf1tm1d(EUCOMM)Wtsi/Brpf1tm1d(EUCOMM)Wtsi (more )
Structure Level Pattern Image Note
TS15: future midbrain Absent 6B Brpf1-null , 6B Brpf1-null/DAPI No evidence of apoptosis at E9.5.
Specimen 6C Control: embryonic day 10.5; Brpf1tm1c(EUCOMM)Wtsi/Brpf1+ (more )
Structure Level Pattern Image Note
TS17: forebrain Absent 6C Control/DAPI , 6C Control
TS17: hindbrain Absent 6C Control/DAPI , 6C Control
TS17: tail Absent 6C Control/DAPI , 6C Control
Specimen 6C Brdf1-null: embryonic day 10.5; Brpf1tm1d(EUCOMM)Wtsi/Brpf1tm1d(EUCOMM)Wtsi (more )
Structure Level Pattern Image Note
TS17: forebrain Present 6C Brpf1-null/DAPI , 6C Brpf1-null Marked apoptosis evident in forebrain, hindbrain and tail region.
TS17: hindbrain Present 6C Brpf1-null/DAPI , 6C Brpf1-null Marked apoptosis evident in forebrain, hindbrain and tail region.
TS17: tail Present 6C Brpf1-null/DAPI , 6C Brpf1-null Marked apoptosis evident in forebrain, hindbrain and tail region.
Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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05/21/2024
MGI 6.23 		The Jackson Laboratory