Gene Expression Data
Assay Details
Assay
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Reference:
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J:177404
Schneider M, et al., Cell-specific detection of microRNA expression during cardiomyogenesis by combined in situ hybridization and immunohistochemistry. J Mol Histol. 2011 Aug;42(4):289-99
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Assay type:
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Immunohistochemistry
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MGI Accession ID:
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MGI:7442568
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Gene symbol:
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Col1a1
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Gene name:
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collagen, type I, alpha 1
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Antibody:
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anti-Col1 (ab34710)
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Detection system:
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Secondary antibody coupled to Alexa Fluor
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Assay notes:
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Antibody recognizes both Col1a1 and Col1a2.
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Results
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Specimen
5 left:
postnatal day 98
(more )
Note: Double labeled: magenta - Col1a1 and Col1a2; green - Acta2 (assay MGI:7442567).
Specimen
5 left:
(close )
Genetic Background:
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C57BL/6
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Age:
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postnatal day 98
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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Formalin
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Embedding:
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Cryosection
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Note:
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Double labeled: magenta - Col1a1 and Col1a2; green - Acta2 (assay MGI:7442567).
|
Structure |
Level |
Pattern |
Image |
Note |
TS28: extracellular matrix |
Present |
Regionally restricted |
5_merge left
,
5_collagen-1 left
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Expression was detected in the extracellular matrix in the heart ventricle.
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Specimen
5 center:
postnatal day 98
(more )
Note: Triple labeled: magenta - Col1a1 and Col1a2; green - Acta2 (assay MGI:7442567); red - Mir125b-1 (assay MGI:7442446) and Mir125b-2 (assay MGI:7442492).
Specimen
5 center:
(close )
Genetic Background:
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C57BL/6
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Age:
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postnatal day 98
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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Formalin
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Embedding:
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Cryosection
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Note:
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Triple labeled: magenta - Col1a1 and Col1a2; green - Acta2 (assay MGI:7442567); red - Mir125b-1 (assay MGI:7442446) and Mir125b-2 (assay MGI:7442492).
|
Structure |
Level |
Pattern |
Image |
Note |
TS28: extracellular matrix |
Present |
Regionally restricted |
5_collagen-1 center
,
5_merge center
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Expression was detected in the extracellular matrix in the heart ventricle.
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Specimen
5 right:
postnatal day 98
(more )
Note: Triple labeled: magenta - Col1a1 and Col1a2; green - Acta2 (assay MGI:7442567); red - Mir199a-1 (assay MGI:7442494) and Mir199a-2 (assay MGI:7442512).
Specimen
5 right:
(close )
Genetic Background:
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C57BL/6
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Age:
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postnatal day 98
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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Formalin
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Embedding:
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Cryosection
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Note:
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Triple labeled: magenta - Col1a1 and Col1a2; green - Acta2 (assay MGI:7442567); red - Mir199a-1 (assay MGI:7442494) and Mir199a-2 (assay MGI:7442512).
|
Structure |
Level |
Pattern |
Image |
Note |
TS28: extracellular matrix |
Present |
Regionally restricted |
5_collagen-1 right
,
5_merge right
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Expression was detected in the extracellular matrix in the heart ventricle.
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