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Gene Expression Data
Assay Details
Assay
Reference: J:177404 Schneider M, et al., Cell-specific detection of microRNA expression during cardiomyogenesis by combined in situ hybridization and immunohistochemistry. J Mol Histol. 2011 Aug;42(4):289-99
Assay type: RNA in situ
MGI Accession ID: MGI:7442446
Gene symbol: Mir125b-1
Gene name: microRNA 125b-1
Probe: miR-125b probe
Probe preparation: Antisense labelled with digoxigenin RNA
Visualized with: Rhodamine
Assay notes: The mature microRNA is encoded at multiple sites in the genome.
Results
Specimen 3 miR-125b: embryonic day 14.5 (more )
Note: Double labeled: red - Mir125b-1 and Mir125b-2; green - Myosin.
Structure Level Pattern Image Note
TS22: heart Present Regionally restricted 3_miR-125b Expression was widely distributed within connective tissues of the heart.
TS22: dorsal aorta Present Regionally restricted 3_miR-125b Expression was localized to the aortic wall.
TS22: atrioventricular septal complex Present Regionally restricted 3_miR-125b Expression was detected in streaks of connective tissue in the atrioventricular junction.
Specimen 4b: postnatal day 98 (more )
Note: Double labeled: red - Mir125b-1 and Mir125b-2; green - Myosin.
Structure Level Pattern Image Note
TS28: artery Present Regionally restricted 4b* , 4b Expression was detected in cardiac arteries.
TS28: cardiac interstitium Present 4b* , 4b
Specimen 4e: postnatal day 98 (more )
Note: Double labeled: red - Mir125b-1 and Mir125b-2; green - Myosin.
Structure Level Pattern Image Note
TS28: cardiac interstitium Present 4e , 4e*
TS28: visceral pericardium Present 4e* , 4e Expression was detected in epicardial cells.
Specimen 4h: postnatal day 98 (more )
Note: Double labeled: red - Mir125b-1 and Mir125b-2; green - Myosin.
Structure Level Pattern Image Note
TS28: aorta wall Present 4h* , 4h
TS28: aortic valve Present 4h* , 4h
TS28: cardiac interstitium Present 4h* , 4h
TS28: heart valve Present 4h* , 4h
Specimen 4k: postnatal day 98 (more )
Note: Double labeled: red - Mir125b-1 and Mir125b-2; green - Myosin.
Structure Level Pattern Image Note
TS28: visceral pericardium Present 4kR+B+G , 4kR+B , 4kR Expression was detected in epicardial cells.
Specimen 4n: postnatal day 98 (more )
Note: Double labeled: red - Mir125b-1 and Mir125b-2; green - Myosin.
Structure Level Pattern Image Note
TS28: endocardial lining Ambiguous 4nR , 4nR+B , 4nR+B+G
Specimen 5 center: postnatal day 98 (more )
Note: Triple labeled: red - Mir125b-1 and Mir125b-2; green - Acta2 (assay MGI:7442567); magenta - Col1a1 (assay MGI:7442568) and Col1a2 (assay MGI:7442583).
Structure Cell Type Level Pattern Image Note
TS28: extracellular matrix Present Regionally restricted 5_merge center , 5_miR-125b In the heart ventricle, expression localized in Acta2-negative perivascular cells embedded in the Collagen-I-positive extracellular matrix .
TS28: heart ventricle smooth muscle cell Absent 5_merge center , 5_miR-125b Acta2-positive smooth muscle cells showed no expression.
Specimen Table 1 E14.5: embryonic day 14.5 (more )
Structure Cell Type Level Pattern Image Note
TS22: cardiac muscle tissue cardiac muscle cell Weak
TS22: heart Strong Regionally restricted Strong expression was detected in connective tissue.
TS22: connective tissue Strong Regionally restricted Strong expression was detected in connective tissue of several organs.
Specimen Table 1 P98: postnatal day 98 (more )
Structure Cell Type Level Pattern Image Note
TS28: cardiac muscle tissue cardiac muscle cell Absent
TS28: aorta wall Strong
TS28: aortic valve Strong
TS28: cardiac interstitium Strong
TS28: heart valve Strong
TS28: visceral pericardium Strong
TS28: artery Strong Regionally restricted Expression was detected in Acta2-negative cells of vessel walls.
Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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05/21/2024
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