About   Help   FAQ
Gene Expression Data
Assay Details
Assay
Reference: J:294158 Vernersson Lindahl E, et al., An allelic series of Trp63 mutations defines TAp63 as a modifier of EEC syndrome. Am J Med Genet A. 2013 Aug;161A(8):1961-71
Assay type: RT-PCR
MGI Accession ID: MGI:7438173
Gene symbol: Trp63
Gene name: transformation related protein 63
Probe: p63-ex6, p63-ex8
Assay notes: Wild type and mutant transcripts can be differentiated by the presence or absence, respectively, of a BsaHI site in exon 7. The products of the RT-PCR reaction were subjected to restriction enzyme digestion with BsaHI to generate either two wild type fragments (213 bp and 135 bp) or one mutant fragment (348 bp).
Results Image: 5d
 Sample Information Bands Other Sample Information
Lane AgeStructure 348 bp (a) 213 bp (b) 135 bp (b) Amount Genetic BackgroundMutant Allele(s)Sex
R279H +/+ P newborn TS27: epidermis Not Applicable Present Present Not Specified; total RNA involves: 129S7/SvEvBrd * BALB/c * C57BL/6 Not Specified
R279H +/- P newborn TS27: epidermis Present Present Present Not Specified; total RNA involves: 129S7/SvEvBrd * BALB/c * C57BL/6 Trp63tm3.1Aam/Trp63+Not Specified
R279H +/- P newborn TS27: epidermis Present Present Present Not Specified; total RNA involves: 129S7/SvEvBrd * BALB/c * C57BL/6 Trp63tm3.1Aam/Trp63+Not Specified
R279H +/- P newborn TS27: epidermis Present Present Present Not Specified; total RNA involves: 129S7/SvEvBrd * BALB/c * C57BL/6 Trp63tm3.1Aam/Trp63+Not Specified
R279H +/- P newborn TS27: epidermis Present Present Present Not Specified; total RNA involves: 129S7/SvEvBrd * BALB/c * C57BL/6 Trp63tm3.1Aam/Trp63+Not Specified
R279H +/- P newborn TS27: epidermis Present Present Present Not Specified; total RNA involves: 129S7/SvEvBrd * BALB/c * C57BL/6 Trp63tm3.1Aam/Trp63+Not Specified
R279H +/- P newborn TS27: epidermis Present Present Present Not Specified; total RNA involves: 129S7/SvEvBrd * BALB/c * C57BL/6 Trp63tm3.1Aam/Trp63+Not Specified
R279HN +/+ P newborn TS27: epidermis Not Applicable Present Present Not Specified; total RNA involves: 129S7/SvEvBrd Not Specified
R279HN +/- P newborn TS27: epidermis Present Present Present Not Specified; total RNA involves: 129S7/SvEvBrd Trp63tm3Aam/Trp63+Not Specified
R279HN +/- P newborn TS27: epidermis Present Present Present Not Specified; total RNA involves: 129S7/SvEvBrd Trp63tm3Aam/Trp63+Not Specified
R279HN +/- P newborn TS27: epidermis Present Present Present Not Specified; total RNA involves: 129S7/SvEvBrd Trp63tm3Aam/Trp63+Not Specified
R279HN +/- P newborn TS27: epidermis Present Present Present Not Specified; total RNA involves: 129S7/SvEvBrd Trp63tm3Aam/Trp63+Not Specified
R279HN +/- P newborn TS27: epidermis Present Present Present Not Specified; total RNA involves: 129S7/SvEvBrd Trp63tm3Aam/Trp63+Not Specified
R279HN +/- P newborn TS27: epidermis Present Present Present Not Specified; total RNA involves: 129S7/SvEvBrd Trp63tm3Aam/Trp63+Not Specified
R279HN +/- P newborn TS27: epidermis Present Present Present Not Specified; total RNA involves: 129S7/SvEvBrd Trp63tm3Aam/Trp63+Not Specified
R279HN +/- P newborn TS27: epidermis Present Present Present Not Specified; total RNA involves: 129S7/SvEvBrd Trp63tm3Aam/Trp63+Not Specified
Notes:
(a) mutant restriction fragment. Trp63<R279HN/+> mice expressed a higher proportion of mutant Trp63 transcript compared to Trp63<R279H/+> mice. Mutant transcript accounted for 50% of the total Trp63 transcript pool in Trp63<R279HN/+> epidermis compared to only ~8% in the Trp63<R279H/+> epidermis.
(b) wild type restriction fragment
Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
Citing These Resources
Funding Information
Warranty Disclaimer, Privacy Notice, Licensing, & Copyright
Send questions and comments to User Support. 		last database update
12/30/2025
MGI 6.24 		The Jackson Laboratory