Pcna Immunohistochemistry Gene Expression Assay

Gene Expression Data
Assay Details

Assay

Reference:	J:283394 Hughes LJ, et al., Yap/Taz are required for establishing the cerebellar radial glia scaffold and proper foliation. Dev Biol. 2020 Jan 1;457(1):150-162
Assay type:	Immunohistochemistry
MGI Accession ID:	MGI:7434459
Gene symbol:	Pcna
Gene name:	proliferating cell nuclear antigen

Antibody:	anti-PCNA (10205-2-AP)
Detection system:	Secondary antibody coupled to Cy3

Results

3E ©

Specimen 3E: embryonic day 13.5 (more

)
Note: Double labeled: red - Pcna; green - BrdU.

Structure	Level	Pattern	Image	Note
TS21: cerebellum layer	Present	Regionally restricted	3E	Expressed in the cerebellum external granule cell layer.
TS21: upper rhombic lip	Present		3E

3F ©

Specimen 3F: embryonic day 13.5; Tg(Nes-cre)1Atp/0, Wwtr1^tm1.1Eno/Wwtr1^tm1.1Eno, Yap1^tm1.1Eno/Yap1^tm1.1Eno (more

)
Note: Conditional mutant. Double labeled: red - Pcna; green - BrdU.

Structure	Level	Pattern	Image	Note
TS21: cerebellum layer	Present	Regionally restricted	3F	Expressed in the cerebellum external granule cell layer. The proportion of BrdU labeled S-phase cells among total Pcna+ cells was similar as in the wild-type.
TS21: upper rhombic lip	Present		3F	The proportion of BrdU labeled S-phase cells among total Pcna+ cells was similar as in the wild-type.

Contributing Projects: Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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