Antibody:

anti-Sox10 (N-20, sc-17342)

Structure	Cell Type	Level	Pattern	Image	Note
TS15: migrating neural crest	migratory neural crest cell	Present	Regionally restricted	S2Ai	Expression in sacral neural crest cells emigrating from dorsal neural tube adjacent to somite 25.

Structure	Cell Type	Level	Pattern	Image	Note
TS15: migrating neural crest	migratory neural crest cell	Weak	Regionally restricted	S2Aii	Expression in fewer sacral neural crest cells emigrating from neural tube caudal to somite 24 than in wildtype.

Structure	Cell Type	Level	Pattern	Image	Note
TS15: migrating neural crest	migratory neural crest cell	Present	Regionally restricted	S2Aiii	Expression in fewer sacral neural crest cells emigrating from neural tube caudal to somite 24 than in wildtype.

Structure	Cell Type	Level	Pattern	Image	Note
TS17: migrating neural crest	migratory neural crest cell	Present	Regionally restricted	1Ai	Expression in sacral neural crest cells along medial pathway and their pioneer (most ventrally located) cells.

Structure	Cell Type	Level	Pattern	Image	Note
TS17: migrating neural crest	migratory neural crest cell	Weak	Regionally restricted	1Aii	Significant reduction in expressing pioneer sacral neural crest cells in periaortic area compared to wildtype.

Structure	Cell Type	Level	Pattern	Image	Note
TS17: migrating neural crest	migratory neural crest cell	Weak	Regionally restricted	1Aiii	Significant reduction in expressing pioneer sacral neural crest cells in periaortic area compared to wildtype.

Structure	Cell Type	Level	Pattern	Image	Note
TS19: pelvic ganglion	migratory neural crest cell	Present	Regionally restricted	1Di	Expression in sacral neural crest cells - pioneer cells aggregated as pelvic ganglia lateral to distal hindgut.

Structure	Cell Type	Level	Pattern	Image	Note
TS19: pelvic ganglion	migratory neural crest cell	Ambiguous		1Dii	Expressing cells in pelvic ganglion significantly reduced in number.

Structure	Level	Pattern	Image	Note
TS19: pelvic ganglion	Absent		1Diii	Neural crest cells not found in position of pelvic ganglion.

Structure	Cell Type	Level	Pattern	Image	Note
TS20: pelvic ganglion	migratory neural crest cell	Present	Regionally restricted	S2Ci	Expression in sacral neural crest cells.

Structure	Cell Type	Level	Pattern	Image	Note
TS20: pelvic ganglion	migratory neural crest cell	Weak	Regionally restricted	S2Cii	Signficantly fewer sacral neural crest cells than in wildtype.

Structure	Level	Pattern	Image	Note
TS20: pelvic ganglion	Absent		S2Ciii	No co-expression with p75 (Ngfr).

Structure	Cell Type	Level	Pattern	Image	Note
TS21: pelvic ganglion	migratory neural crest cell	Present	Regionally restricted	S2Ei	Expression in sacral neural crest cells.

Structure	Cell Type	Level	Pattern	Image	Note
TS21: pelvic ganglion	migratory neural crest cell	Weak	Regionally restricted	S2Eii	Signficantly fewer sacral neural crest cells than in wildtype.

Structure	Level	Pattern	Image	Note
TS21: pelvic ganglion	Absent		S2Eiii	No co-expression with p75 (Ngfr).

Structure	Cell Type	Level	Pattern	Image	Note
TS22: hindgut	neural crest cell	Present	Regionally restricted	S2Gii , S2Giii , S2Gi	Expression in neural crest cells in middle and distal regions of hindgut segments.
TS22: pelvic ganglion	migratory neural crest cell	Present	Regionally restricted	S2Giii , S2Gi	Expression in sacral neural crest cells.

Structure	Cell Type	Level	Pattern	Image	Note
TS22: hindgut	neural crest cell	Present	Regionally restricted	S2Gvi , S2Gv , S2Giv	Expression in neural crest cells in middle and distal regions of hindgut segments.
TS22: pelvic ganglion	migratory neural crest cell	Weak	Regionally restricted	S2Gvi , S2Giv	Signficantly fewer sacral neural crest cells in pelvic ganglia than in wildtype.

Structure	Cell Type	Level	Pattern	Image	Note
TS22: hindgut	neural crest cell	Present	Regionally restricted	S2Gviii , S2Gvii , S2Gix	Expression in neural crest cells in middle and distal regions of hindgut segments.
TS22: pelvic ganglion	migratory neural crest cell	Absent		S2Gvii , S2Gix	Signficantly fewer sacral neural crest cells in pelvic ganglia than in wildtype.

Structure	Cell Type	Level	Pattern	Image	Note
TS17: migrating neural crest	migratory neural crest cell	Present		S3Aiii

Structure	Cell Type	Level	Pattern	Image	Note
TS17: migrating neural crest	migratory neural crest cell	Present		S3Aiv

Structure	Cell Type	Level	Pattern	Image	Note
TS17: migrating neural crest	migratory neural crest cell	Present		S3Ciii

Structure	Cell Type	Level	Pattern	Image	Note
TS17: migrating neural crest	migratory neural crest cell	Present		S3Civ

Structure	Cell Type	Level	Pattern	Image	Note
TS17: migrating neural crest	migratory neural crest cell	Present		S3E +/+ Sox10 , S3E +/+ Merged

Structure	Cell Type	Level	Pattern	Image	Note
TS17: migrating neural crest	migratory neural crest cell	Present		S3E Dom/Dom Sox10 , S3E Dom/Dom Merged