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Gene Expression Data
Assay Details
Assay
Reference: J:231673 Gustin SE, et al., WNT/beta-catenin and p27/FOXL2 differentially regulate supporting cell proliferation in the developing ovary. Dev Biol. 2016 Apr 15;412(2):250-60
Assay type: Immunohistochemistry
MGI Accession ID: MGI:7432672
Gene symbol: Cdkn1b
Gene name: cyclin dependent kinase inhibitor 1B
Results
Specimen 3B Foxl2+/+ top: embryonic day 13.5 (more )
Note: Double labeled: red - Cdkn1b; green - Foxl2 (assay MGI:7432671).
Structure Level Pattern Image Note
TS21: ovary Present 3B E13.5 XX gonad top Foxl2+/+ Expression was detected in somatic cells.
Specimen 3B Foxl2+/+ bottom: embryonic day 13.5 (more )
Note: Double labeled: red - Cdkn1b; green - Foxl2 (assay MGI:7432671).
Structure Level Pattern Image Note
TS21: ovary Present 3B E13.5 XX gonad bottom Foxl2+/+ Expression was detected in somatic cells.
Specimen 3B Foxl2-/- top: embryonic day 13.5; Foxl2tm1Gpil/Foxl2tm1Gpil (more )
Note: Double labeled: red - Cdkn1b; green - Foxl2 (assay MGI:7432671).
Structure Level Pattern Image Note
TS21: ovary Weak 3B E13.5 XX gonad top Foxl2-/- A substantial reduction of expression level in somatic cells was noted in the mutant.
Specimen 3B Foxl2-/- bottom: embryonic day 13.5; Foxl2tm1Gpil/Foxl2tm1Gpil (more )
Note: Double labeled: red - Cdkn1b; green - Foxl2 (assay MGI:7432671).
Structure Level Pattern Image Note
TS21: ovary Weak 3B E13.5 XX gonad bottom Foxl2-/- A substantial reduction of expression level in somatic cells was noted in the mutant.
Specimen 3B Foxl2+/+ E15.5: embryonic day 15.5 (more )
Note: Double labeled: red - Cdkn1b; green - Foxl2 (assay MGI:7432671).
Structure Level Pattern Image Note
TS23: ovary Present 3B E15.5 XX gonad Foxl2+/+ Expression was detected in somatic cells.
Specimen 3B Foxl2-/- E15.5: embryonic day 15.5; Foxl2tm1Gpil/Foxl2tm1Gpil (more )
Note: Double labeled: red - Cdkn1b; green - Foxl2 (assay MGI:7432671).
Structure Level Pattern Image Note
TS23: ovary Weak 3B E15.5 XX gonad Foxl2-/- A substantial reduction of expression level in somatic cells was noted in the mutant.
Specimen 4A: embryonic day 13.5; Lgr5tm1(cre/ERT2)Cle/Lgr5+ (more )
Note: Triple labeled: red - Cdkn1b; white - Gata4 (assay MGI:7432676); green - Lgr5 reporter (assay MGI:7432735).
Structure Level Pattern Image Note
TS21: ovary Present Regionally restricted 4A left , 4A i , 4A ii , 4A iii (i) GATA4-positive/LGR5GFP-positive/Cdkn1b-negative cells (green cells marked by >), (ii) GATA4-positive/LGR5GFP-positive/Cdkn1b-positive cells (yellow cells marked by arrows) and (iii) GATA4-positive/LRG5GFP-negative/Cdkn1b-positive cells (red cells). Triple positive cells appeared to be populating the medulla of the ovary.
Specimen 4B: embryonic day 16.5; Lgr5tm1(cre/ERT2)Cle/Lgr5+ (more )
Note: Triple labeled: red - Cdkn1b; white - Gata4 (assay MGI:7432676); green - Lgr5 reporter (assay MGI:7432735).
Structure Level Pattern Image Note
TS24: ovary Present Regionally restricted 4B iii , 4B left , 4B i , 4B ii Although some GATA4-positive/p27-positive/Lgr5-GFP-positive cells were also detected in E16.5 ovaries, there was a distinct demarcation between GATA4-positive/p27-negative/Lgr5-GFP-positive cells located in the cortex and the GATA4-positive/p27-positive/Lgr5-GFP-negative cells located in the medulla.
Specimen S2B: embryonic day 12.5 (more )
Note: Triple labeled: red - Cdkn1b; green - Foxl2 (assay MGI:7432671); blue - EdU.
Structure Level Pattern Image Note
TS20: ovary Present S2B left , S2B right Co-localization of signal with Foxl2 was observed.
Specimen S5B XX: embryonic day 11.5 (more )
Note: Triple labeled: red - Cdkn1b; green - Foxl2 (assay MGI:7432671); blue - EdU.
Structure Level Pattern Image Note
TS19: gonad Present S5B XX E11.5 Merge , S5B XX E11.5 p27
TS19: mesonephros Absent S5B XX E11.5 p27 , S5B XX E11.5 Merge
Specimen S5B XY: embryonic day 11.5 (more )
Note: Triple labeled: blue - Cdkn1b; green - Sox9 (assay MGI:7432679); red - EdU.
Structure Level Pattern Image Note
TS19: gonad Present S5B XY E11.5 p27 , S5B XY E11.5 Merge
TS19: mesonephros Absent S5B XY E11.5 p27 , S5B XY E11.5 Merge
Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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05/28/2024
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