About   Help   FAQ
Gene Expression Data
Assay Details
Assay
Reference: J:231673 Gustin SE, et al., WNT/beta-catenin and p27/FOXL2 differentially regulate supporting cell proliferation in the developing ovary. Dev Biol. 2016 Apr 15;412(2):250-60
Assay type: Immunohistochemistry
MGI Accession ID: MGI:7432672
Gene symbol: Cdkn1b
Gene name: cyclin dependent kinase inhibitor 1B
Results
Specimen 3B Foxl2+/+ top: embryonic day 13.5 (more )
Note: Double labeled: red - Cdkn1b; green - Foxl2 (assay MGI:7432671).
Structure Level Pattern Image Note
TS21: ovary Present 3B E13.5 XX gonad top Foxl2+/+ Expression was detected in somatic cells.
Specimen 3B Foxl2+/+ bottom: embryonic day 13.5 (more )
Note: Double labeled: red - Cdkn1b; green - Foxl2 (assay MGI:7432671).
Structure Level Pattern Image Note
TS21: ovary Present 3B E13.5 XX gonad bottom Foxl2+/+ Expression was detected in somatic cells.
Specimen 3B Foxl2-/- top: embryonic day 13.5; Foxl2tm1Gpil/Foxl2tm1Gpil (more )
Note: Double labeled: red - Cdkn1b; green - Foxl2 (assay MGI:7432671).
Structure Level Pattern Image Note
TS21: ovary Weak 3B E13.5 XX gonad top Foxl2-/- A substantial reduction of expression level in somatic cells was noted in the mutant.
Specimen 3B Foxl2-/- bottom: embryonic day 13.5; Foxl2tm1Gpil/Foxl2tm1Gpil (more )
Note: Double labeled: red - Cdkn1b; green - Foxl2 (assay MGI:7432671).
Structure Level Pattern Image Note
TS21: ovary Weak 3B E13.5 XX gonad bottom Foxl2-/- A substantial reduction of expression level in somatic cells was noted in the mutant.
Specimen 3B Foxl2+/+ E15.5: embryonic day 15.5 (more )
Note: Double labeled: red - Cdkn1b; green - Foxl2 (assay MGI:7432671).
Structure Level Pattern Image Note
TS23: ovary Present 3B E15.5 XX gonad Foxl2+/+ Expression was detected in somatic cells.
Specimen 3B Foxl2-/- E15.5: embryonic day 15.5; Foxl2tm1Gpil/Foxl2tm1Gpil (more )
Note: Double labeled: red - Cdkn1b; green - Foxl2 (assay MGI:7432671).
Structure Level Pattern Image Note
TS23: ovary Weak 3B E15.5 XX gonad Foxl2-/- A substantial reduction of expression level in somatic cells was noted in the mutant.
Specimen 4A: embryonic day 13.5; Lgr5tm1(cre/ERT2)Cle/Lgr5+ (more )
Note: Triple labeled: red - Cdkn1b; white - Gata4 (assay MGI:7432676); green - Lgr5 reporter (assay MGI:7432735).
Structure Level Pattern Image Note
TS21: ovary Present Regionally restricted 4A left , 4A i , 4A ii , 4A iii (i) GATA4-positive/LGR5GFP-positive/Cdkn1b-negative cells (green cells marked by >), (ii) GATA4-positive/LGR5GFP-positive/Cdkn1b-positive cells (yellow cells marked by arrows) and (iii) GATA4-positive/LRG5GFP-negative/Cdkn1b-positive cells (red cells). Triple positive cells appeared to be populating the medulla of the ovary.
Specimen 4B: embryonic day 16.5; Lgr5tm1(cre/ERT2)Cle/Lgr5+ (more )
Note: Triple labeled: red - Cdkn1b; white - Gata4 (assay MGI:7432676); green - Lgr5 reporter (assay MGI:7432735).
Structure Level Pattern Image Note
TS24: ovary Present Regionally restricted 4B iii , 4B left , 4B i , 4B ii Although some GATA4-positive/p27-positive/Lgr5-GFP-positive cells were also detected in E16.5 ovaries, there was a distinct demarcation between GATA4-positive/p27-negative/Lgr5-GFP-positive cells located in the cortex and the GATA4-positive/p27-positive/Lgr5-GFP-negative cells located in the medulla.
Specimen S2B: embryonic day 12.5 (more )
Note: Triple labeled: red - Cdkn1b; green - Foxl2 (assay MGI:7432671); blue - EdU.
Structure Level Pattern Image Note
TS20: ovary Present S2B left , S2B right Co-localization of signal with Foxl2 was observed.
Specimen S5B XX: embryonic day 11.5 (more )
Note: Triple labeled: red - Cdkn1b; green - Foxl2 (assay MGI:7432671); blue - EdU.
Structure Level Pattern Image Note
TS19: gonad Present S5B XX E11.5 Merge , S5B XX E11.5 p27
TS19: mesonephros Absent S5B XX E11.5 p27 , S5B XX E11.5 Merge
Specimen S5B XY: embryonic day 11.5 (more )
Note: Triple labeled: blue - Cdkn1b; green - Sox9 (assay MGI:7432679); red - EdU.
Structure Level Pattern Image Note
TS19: gonad Present S5B XY E11.5 p27 , S5B XY E11.5 Merge
TS19: mesonephros Absent S5B XY E11.5 p27 , S5B XY E11.5 Merge
Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
Citing These Resources
Funding Information
Warranty Disclaimer, Privacy Notice, Licensing, & Copyright
Send questions and comments to User Support. 		last database update
03/18/2025
MGI 6.24 		The Jackson Laboratory