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Gene Expression Data
Assay Details
Assay
Reference: J:220452 Nasif S, et al., Islet 1 specifies the identity of hypothalamic melanocortin neurons and is critical for normal food intake and adiposity in adulthood. Proc Natl Acad Sci U S A. 2015 Apr 14;112(15):E1861-70
Assay type: Immunohistochemistry
MGI Accession ID: MGI:7432569
Gene symbol: Isl1
Gene name: ISL1 transcription factor, LIM/homeodomain
Antibody: clone 39.4D5
Detection system: Secondary antibody coupled to Alexa Fluor
Results
Specimen 1A E9.5: embryonic day 9.5 (more )
Note: Double labeled: red - Isl1; green - Pomc (assay MGI:7432658).
Structure Level Pattern Image Note
TS15: future hindbrain Present 1A Isl1 E9.5 , 1A E9.5 Overlay
Specimen 1A E10.0: embryonic day 10.0 (more )
Note: Double labeled: red - Isl1; green - Pomc (assay MGI:7432658).
Structure Level Pattern Image Note
TS16: diencephalon Present Single cells 1A Isl1 E10.0 , 1A E10.0 Overlay Expression is in the future hypothalamus.
TS16: 1st branchial arch Strong 1A Isl1 E10.0 , 1A E10.0 Overlay
TS16: Rathke's pouch Strong 1A Isl1 E10.0 , 1A E10.0 Overlay
Specimen 1A E10.5: embryonic day 10.5 (more )
Note: Double labeled: red - Isl1; green - Pomc (assay MGI:7432658).
Structure Level Pattern Image Note
TS17: diencephalon Present Regionally restricted 1A E10.5 Overlay , 1A Isl1 E10.5 Expression is in the mantle zone of the future hypothalamus.
Specimen 1A E12.5: embryonic day 12.5 (more )
Note: Double labeled: red - Isl1; green - Pomc (assay MGI:7432658).
Structure Level Pattern Image Note
TS20: hypothalamus mantle layer Present 1A Isl1 E12.5 , 1A E12.5 Overlay
Specimen 5A Control: embryonic day 11.5 (more )
Structure Level Pattern Image Note
TS19: diencephalon Moderate Regionally restricted 5A Isl1 Control Expression is in the developing hypothalamus.
Specimen 5A Ascl1-/-: embryonic day 11.5; Ascl1tm1And/Ascl1tm1And (more )
Structure Level Pattern Image Note
TS19: diencephalon Present Regionally restricted 5A Isl1 Ascl1-/- Expression is in the developing hypothalamus and in this mutant is reduced to approximately 10% of that of wild-type levels.
Specimen 5A Ngn3-/-: embryonic day 11.5; Neurog3tm1Fgu/Neurog3tm1Fgu (more )
Structure Level Pattern Image Note
TS19: diencephalon Present Regionally restricted 5A Isl1 Ngn3-/- Expression is in the developing hypothalamus and is similar to wild-type.
Specimen 5A Ascl1-/-;Ngn3-/-: embryonic day 11.5; Ascl1tm1And/Ascl1tm1And, Neurog3tm1Fgu/Neurog3tm1Fgu (more )
Structure Level Pattern Image Note
TS19: diencephalon Present Regionally restricted 5A Isl1 Ascl1-/-;Ngn3-/- Expression is in the developing hypothalamus and in this mutant is reduced to approximately 10% of that of wild-type levels.
Specimen S4B: embryonic day 11.5 (more )
Note: Double labeled: red - Isl1; green - Otp (assay MGI:7432587).
Structure Level Pattern Image Note
TS19: diencephalon Present Regionally restricted S4B Overlay , S4B ISL1 , S4B ISL1/OTP Expression is in the future hypothalamus.
TS19: hindbrain Absent S4B Overlay , S4B ISL1 , S4B ISL1/OTP
Specimen S5A: embryonic day 11.5 (more )
Note: Double labeled: green - Isl1; red - Ascl1 (assay MGI:7432597).
Structure Level Pattern Image Note
TS19: diencephalon Present Regionally restricted S5A ISL1 , S5A Overlay Expression is in the future hypothalamus.
Specimen S5B: embryonic day 11.5 (more )
Note: Double labeled: green - Isl1; red - Neurog3 (assay MGI:7432606).
Structure Level Pattern Image Note
TS19: diencephalon Present Regionally restricted S5B ISL1 , S5B Overlay Expression is in the future hypothalamus.
Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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03/18/2025
MGI 6.24 		The Jackson Laboratory