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Gene Expression Data
Assay Details
Assay
Reference: J:220452 Nasif S, et al., Islet 1 specifies the identity of hypothalamic melanocortin neurons and is critical for normal food intake and adiposity in adulthood. Proc Natl Acad Sci U S A. 2015 Apr 14;112(15):E1861-70
Assay type: Immunohistochemistry
MGI Accession ID: MGI:7432658
Gene symbol: Pomc
Gene name: pro-opiomelanocortin-alpha
Antibody: anti-ACTH (AFP-156102789)
Detection system: Secondary antibody coupled to Alexa Fluor
Results
Specimen 1A E9.5: embryonic day 9.5 (more )
Note: Double labeled: green - Pomc; red - Isl1 (assay MGI:7432569).
Structure Level Pattern Image Note
TS15: future hindbrain Present 1A ACTH E9.5 , 1A E9.5 Overlay
TS15: diencephalon Absent 1A E9.5 Overlay , 1A ACTH E9.5
Specimen 1A E10.0: embryonic day 10.0 (more )
Note: Double labeled: green - Pomc; red - Isl1 (assay MGI:7432569).
Structure Level Pattern Image Note
TS16: 1st branchial arch Absent 1A E10.0 Overlay , 1A ACTH E10.0
TS16: diencephalon Absent 1A E10.0 Overlay , 1A ACTH E10.0
Specimen 1A E10.5: embryonic day 10.5 (more )
Note: Double labeled: green - Pomc; red - Isl1 (assay MGI:7432569).
Structure Level Pattern Image Note
TS17: diencephalon Present Regionally restricted 1A E10.5 Overlay , 1A ACTH E10.5 Expression is in the mantle zone of the future hypothalamus.
Specimen 1A E12.5: embryonic day 12.5 (more )
Note: Double labeled: green - Pomc; red - Isl1 (assay MGI:7432569).
Structure Level Pattern Image Note
TS20: hypothalamus mantle layer Present 1A ACTH E12.5 , 1A E12.5 Overlay
Specimen 5A Control: embryonic day 11.5 (more )
Structure Level Pattern Image Note
TS19: diencephalon Moderate Regionally restricted 5A ACTH Control Expression is in the developing hypothalamus.
Specimen 5A Ascl1-/-: embryonic day 11.5; Ascl1tm1And/Ascl1tm1And (more )
Structure Level Pattern Image Note
TS19: diencephalon Present Single cells 5A ACTH Ascl1-/- This mutant only has a few positive cells in the developing hypothalamus.
Specimen 5A Ngn3-/-: embryonic day 11.5; Neurog3tm1Fgu/Neurog3tm1Fgu (more )
Structure Level Pattern Image Note
TS19: diencephalon Present Single cells 5A ACTH Ngn3-/- This mutant only has a few positive cells in the developing hypothalamus.
Specimen 5A Ascl1-/-;Ngn3-/-: embryonic day 11.5; Ascl1tm1And/Ascl1tm1And, Neurog3tm1Fgu/Neurog3tm1Fgu (more )
Structure Level Pattern Image Note
TS19: diencephalon Absent 5A ACTH Ascl1-/-;Ngn3-/- In this mutant expression is absent in the developing hypothalamus.
Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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Send questions and comments to User Support. 		last database update
03/18/2025
MGI 6.24 		The Jackson Laboratory