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Gene Expression Data
Assay Details
Assay
Reference: J:120320 Shih HP, et al., Cranial muscle defects of Pitx2 mutants result from specification defects in the first branchial arch. Proc Natl Acad Sci U S A. 2007 Apr 3;104(14):5907-12
Assay type: In situ reporter (knock in)
MGI Accession ID: MGI:7430765
Gene symbol: Pitx2
Gene name: paired-like homeodomain transcription factor 2
Results
Specimen 2A: embryonic day 9.5; Pitx2tm1Rsd/Pitx2+ (more )
Note: Double labeled: blue - Pitx2 reporter; green - TUNEL; red - PH3.
Structure Level Pattern Image Note
TS15: 1st branchial arch Present 2A , 2B
Specimen 2E: embryonic day 9.5; Pitx2tm1Rsd/Pitx2tm1Rsd (more )
Note: Double labeled: blue - Pitx2 reporter; green - TUNEL; red - PH3.
Structure Level Pattern Image Note
TS15: 1st branchial arch Present 2F , 2E
Specimen 2I: embryonic day 12.0; Pitx2tm1Rsd/Pitx2+ (more )
Note: Triple labeled: blue - Pitx2 reporter; red - Myog (assay MGI:7430769); green - BrdU.
Structure Level Pattern Image Note
TS19: muscle tissue Present Regionally restricted 2I Myog+/Pitx2+ muscle anlagen was detected in the jaw.
Specimen 2J: embryonic day 12.5; Pitx2tm1Rsd/Pitx2+ (more )
Note: Double labeled: red - Pitx2 reporter; green - TUNEL
Structure Level Pattern Image Note
TS20: muscle tissue Present Regionally restricted 2J Expression was detected in muscle anlagen of the jaw.
Specimen 2K: embryonic day 10.0; Pitx2tm1Rsd/Pitx2+ (more )
Note: Double labeled: green - Pitx2 reporter; red - Myf5 (assay MGI:7430770).
Structure Level Pattern Image Note
TS16: 1st branchial arch mesenchyme Present Regionally restricted 2K Co-expression of Myf5 and Pitx2 (beta-gal) was detected in the first branchial arch muscle anlagen.
Specimen 2L: embryonic day 10.5; Pitx2tm1Rsd/Pitx2+ (more )
Note: Triple labeled: blue - Pitx2 reporter; green - Six2 (assay MGI:7430776); red - Myod1 (assay MGI:7430771).
Structure Level Pattern Image Note
TS17: 1st branchial arch mesenchyme Present Regionally restricted 2L Co-expression of Myod1 and Pitx2 (beta-gal) was detected in the first branchial arch muscle anlagen.
Specimen 2M: embryonic day 12.0; Pitx2tm1Rsd/Pitx2tm1Rsd (more )
Note: Triple labeled: blue - Pitx2 reporter; red - Myog (assay MGI:7430769); green - BrdU.
Structure Level Pattern Image Note
TS19: muscle tissue Weak Regionally restricted 2M Myog+/Pitx2+ muscle anlagen was severely reduced or absent in the mutant vestigial jaw.
Specimen 2N: embryonic day 12.5; Pitx2tm1Rsd/Pitx2tm1Rsd (more )
Note: Double labeled: red - Pitx2 reporter; green - TUNEL
Structure Level Pattern Image Note
TS20: muscle tissue Weak Regionally restricted 2N Staining in muscle anlagen was severely reduced or absent in the mutant vestigial jaw.
Specimen 2O: embryonic day 10.0; Pitx2tm1Rsd/Pitx2tm1Rsd (more )
Note: Double labeled: green - Pitx2 reporter; red - Myf5 (assay MGI:7430770).
Structure Level Pattern Image Note
TS16: 1st branchial arch mesenchyme Present Regionally restricted 2O Expression was detected in the mutant residual first branchial arch muscle anlagen.
Specimen 2P: embryonic day 10.5; Pitx2tm1Rsd/Pitx2tm1Rsd (more )
Note: Triple labeled: blue - Pitx2 reporter; green - Six2 (assay MGI:7430776); red - Myod1 (assay MGI:7430771).
Structure Level Pattern Image Note
TS17: 1st branchial arch mesenchyme Present Regionally restricted 2P Expression was detected in the mutant residual first branchial arch muscle anlagen. The fraction of Pitx2(beta-gal)+ cells that was Myod1+ was dramatically reduced in the mutant.
Specimen 2Q: embryonic day 10.5; Pitx2tm1Rsd/Pitx2+, H2az2Tg(Wnt1-cre)11Rth/0, Gt(ROSA)26Sortm1Sor/Gt(ROSA)26Sor+ (more )
Note: Conditional mutant. Triple labeled: blue - Pitx2 reporter; red - Tbx1 (assay MGI:7430778); green - Cre reporter.
Structure Level Pattern Image Note
TS17: 1st branchial arch mesenchyme Present Regionally restricted 2R , 2Q Pitx2(beta-gal) and Tbx1 label identical cell populations in the mesodermal cores of the first and second branchial arches.
TS17: 2nd branchial arch mesenchyme Present Regionally restricted 2R , 2Q Pitx2(beta-gal) and Tbx1 label identical cell populations in the mesodermal cores of the first and second branchial arches.
Specimen 2S: embryonic day 10.5; Pitx2tm1Rsd/Pitx2+, H2az2Tg(Wnt1-cre)11Rth/0, Gt(ROSA)26Sortm1Sor/Gt(ROSA)26Sor+ (more )
Note: Conditional mutant. Triple labeled: blue - Pitx2 reporter; red - Tcf21 (assay MGI:7430779); green - Cre reporter.
Structure Level Pattern Image Note
TS17: 1st branchial arch mesenchyme Present Regionally restricted 2S , 2T Pitx2(beta-gal) and Tcf21 are co-expressed in the mesodermal cores of the first and second branchial arches.
TS17: 2nd branchial arch mesenchyme Present Regionally restricted 2S , 2T Pitx2(beta-gal) and Tcf21 are co-expressed in the mesodermal cores of the first and second branchial arches.
Specimen 2U: embryonic day 10.5; Pitx2tm1Rsd/Pitx2tm1Rsd, H2az2Tg(Wnt1-cre)11Rth/0, Gt(ROSA)26Sortm1Sor/Gt(ROSA)26Sor+ (more )
Note: Conditional mutant. Triple labeled: blue - Pitx2 reporter; red - Tbx1 (assay MGI:7430778); green - Cre reporter.
Structure Level Pattern Image Note
TS17: 1st branchial arch mesenchyme Present Regionally restricted 2V , 2U The Pitx2(beta-gal)+/Tbx1+ cell population was severely reduced in size in the first branchial arch of mutants.
TS17: 2nd branchial arch mesenchyme Present Regionally restricted 2V , 2U The Pitx2(beta-gal)+/Tbx1+ cell population in the second branchial arch showed no significant difference in size.
Specimen 2W: embryonic day 10.5; Pitx2tm1Rsd/Pitx2tm1Rsd, H2az2Tg(Wnt1-cre)11Rth/0, Gt(ROSA)26Sortm1Sor/Gt(ROSA)26Sor+ (more )
Note: Conditional mutant. Triple labeled: blue - Pitx2 reporter; red - Tcf21 (assay MGI:7430779); green - Cre reporter.
Structure Level Pattern Image Note
TS17: 1st branchial arch mesenchyme Present Regionally restricted 2X , 2W The residual Pitx2(beta-gal)+ cell population was severely reduced in size in the first branchial arch of mutants, and no Tcf21+ colabel was noted.
TS17: 2nd branchial arch mesenchyme Present Regionally restricted 2X , 2W The Pitx2(beta-gal)+/Tcf1+ cell population in the second branchial arch showed no significant difference in size.
Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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03/25/2025
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