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Gene Expression Data
Assay Details
Assay
Reference: J:253585 Siletti K, et al., Daple coordinates organ-wide and cell-intrinsic polarity to pattern inner-ear hair bundles. Proc Natl Acad Sci U S A. 2017 Dec 26;114(52):E11170-E11179
Assay type: Immunohistochemistry
MGI Accession ID: MGI:7427533
Gene symbol: Gnai3
Gene name: G protein subunit alpha i3
Antibody: Anti-G-alpha-i3 (G4040)
Detection system: Secondary antibody
Assay notes: The authors used three anti-Gnai3 antibodies but did not detail which was used to obtain these data; anti-Gai-3 (sc-262) (sc-137416) MGI:7427459 or anti-GNAI3 MGI:7427460 may have been used instead to generate these data.
Results
Specimen 2C: postnatal day 1; Ccdc88ctm1(KOMP)Mbp/Ccdc88c+ (more )
Note: Triple labeled: magenta - Gnai3; green - Tubulin; gray - phalloidin.
Structure Level Pattern Image Note
TS27: cochlea Present Regionally restricted 2C Kinocilium is centered within domain of Gnai3 expression.
Specimen 2D: postnatal day 1; Ccdc88ctm1(KOMP)Mbp/Ccdc88ctm1(KOMP)Mbp (more )
Note: Triple labeled: magenta - Gnai3; green - Tubulin; gray - phalloidin.
Structure Cell Type Level Pattern Image Note
TS27: cochlea cochlea auditory hair cell Present Regionally restricted 2D Kinocilium located at end of expression domain in hair cell with S-shaped bundle.
Specimen 2E: postnatal day 1; Ccdc88ctm1(KOMP)Mbp/Ccdc88ctm1(KOMP)Mbp (more )
Note: Triple labeled: magenta - Gnai3; green - Tubulin; gray - phalloidin.
Structure Cell Type Level Pattern Image Note
TS27: cochlea cochlea auditory hair cell Present Regionally restricted 2E Kinocilium near center of hair cell with C-shaped bundle.
Specimen 2F: postnatal day 1; Ccdc88ctm1(KOMP)Mbp/Ccdc88ctm1(KOMP)Mbp (more )
Note: Triple labeled: magenta - Gnai3; green - Tubulin; gray - phalloidin.
Structure Cell Type Level Pattern Image Note
TS27: cochlea cochlea auditory hair cell Present Regionally restricted 2F Kinocilium centered within domain of expression in hair cells resembling control.
Specimen 2G: postnatal day 1; Ccdc88ctm1(KOMP)Mbp/Ccdc88ctm1(KOMP)Mbp (more )
Note: Triple labeled: magenta - Gnai3; green - Tubulin; gray - phalloidin.
Structure Cell Type Level Pattern Image Note
TS27: cochlea cochlea auditory hair cell Present Regionally restricted 2G Kinocilium opposes crescent of Gnai3 expression in hair cell with fragmented bundle.
Specimen 3A Base Daple+/-: postnatal day 2; Ccdc88ctm1(KOMP)Mbp/Ccdc88c+ (more )
Note: Triple labeled: magenta - Gnai3; green - Tubulin; gray - phalloidin.
Structure Cell Type Level Pattern Image Note
TS27: cochlea cochlea auditory hair cell Present Regionally restricted 3A Base Daple+/- Expression at apical surface of hair cells.
Specimen 3A Base Daple-/-: postnatal day 2; Ccdc88ctm1(KOMP)Mbp/Ccdc88ctm1(KOMP)Mbp (more )
Note: Triple labeled: magenta - Gnai3; green - Tubulin; gray - phalloidin.
Structure Cell Type Level Pattern Image Note
TS27: cochlea cochlea auditory hair cell Present Regionally restricted 3A Base Daple-/- Expression at apical surface of hair cells.
Specimen 3A Apex Daple+/-: postnatal day 2; Ccdc88ctm1(KOMP)Mbp/Ccdc88c+ (more )
Note: Triple labeled: magenta - Gnai3; green - Tubulin; gray - phalloidin.
Structure Cell Type Level Pattern Image Note
TS27: cochlea cochlea auditory hair cell Present Regionally restricted 3A Apex Daple+/- Expression at apical surface of hair cells.
Specimen 3A Apex Daple-/-: postnatal day 2; Ccdc88ctm1(KOMP)Mbp/Ccdc88ctm1(KOMP)Mbp (more )
Note: Triple labeled: magenta - Gnai3; green - Tubulin; gray - phalloidin.
Structure Cell Type Level Pattern Image Note
TS27: cochlea cochlea auditory hair cell Present Regionally restricted 3A Apex Daple-/- Expression at apical surface of hair cells.
Specimen 4B: postnatal newborn; Ccdc88ctm1(KOMP)Mbp/Ccdc88ctm1(KOMP)Mbp (more )
Structure Cell Type Level Pattern Image Note
TS27: cochlea cochlear outer hair cell Present Regionally restricted 4B Expression in outer hair cells near apex of cochlea.
Specimen 4D: postnatal newborn; Ccdc88ctm1(KOMP)Mbp/Ccdc88ctm1(KOMP)Mbp (more )
Structure Cell Type Level Pattern Image Note
TS27: cochlea cochlear outer hair cell Present Regionally restricted 4D Expression in outer hair cells near apex of cochlea.
Specimen 8C (top): postnatal day 1; Ccdc88ctm1(KOMP)Mbp/Ccdc88c+ (more )
Note: Double labeled: magenta - Gnai3; green - ZO-1 (Tjp1 assay MGI:7427542).
Structure Level Pattern Image Note
TS27: cochlea Present Regionally restricted 8C (top) Expression excluded from intercellular junctions near apex of cochlea.
Specimen 8C (bottom): postnatal day 1; Ccdc88ctm1(KOMP)Mbp/Ccdc88c+ (more )
Note: Double labeled: magenta - Gnai3; green - ZO-1 (Tjp1 assay MGI:7427542).
Structure Cell Type Level Pattern Image Note
TS27: cochlea cochlea auditory hair cell Present Regionally restricted 8C (bottom) Exprssion restricted to apical surface of hair cells.
Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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05/28/2024
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