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Gene Expression Data
Assay Details
Assay
Reference: J:253585 Siletti K, et al., Daple coordinates organ-wide and cell-intrinsic polarity to pattern inner-ear hair bundles. Proc Natl Acad Sci U S A. 2017 Dec 26;114(52):E11170-E11179
Assay type: Immunohistochemistry
MGI Accession ID: MGI:7427542
Gene symbol: Tjp1
Gene name: tight junction protein 1
Antibody: anti-ZO-1
Detection system: Secondary antibody coupled to Alexa Fluor 488
Assay notes: Fixation was with TCA.
Results
Specimen 7B Daple+/-: postnatal day 0; Ccdc88ctm1(KOMP)Mbp/Ccdc88c+ (more )
Note: Double labeled: green - ZO-1 (Tjp1); magenta - Dvl2 (assay MGI:7427539).
Structure Level Pattern Image Note
TS27: cochlea Present Regionally restricted 7B ZO-1 Daple+/- , 7B Merged Daple+/- Expression at tight junctions near apex of cochlea.
Specimen 7B Daple-/-: postnatal day 0; Ccdc88ctm1(KOMP)Mbp/Ccdc88ctm1(KOMP)Mbp (more )
Note: Double labeled: green - ZO-1 (Tjp1); magenta - Dvl2 (assay MGI:7427539).
Structure Level Pattern Image Note
TS27: cochlea Present Regionally restricted 7B ZO-1 Daple-/- , 7B Merged Daple-/- Expression at tight junctions near apex of cochlea.
Specimen 7C Daple+/-: postnatal day 0; Ccdc88ctm1(KOMP)Mbp/Ccdc88c+ (more )
Note: Double labeled: green - ZO-1 (Tjp1); magenta - Dvl2 (assay MGI:7427539).
Structure Level Pattern Image Note
TS27: cochlea Present Regionally restricted 7C ZO-1 Daple+/- , 7C Merged Daple+/- Expression at tight junctions near base of cochlea.
Specimen 7C Daple-/-: postnatal day 0; Ccdc88ctm1(KOMP)Mbp/Ccdc88ctm1(KOMP)Mbp (more )
Note: Double labeled: green - ZO-1 (Tjp1); magenta - Dvl2 (assay MGI:7427539).
Structure Level Pattern Image Note
TS27: cochlea Present Regionally restricted 7C Merged Daple-/- , 7C ZO-1 Daple-/- Expression at tight junctions near base of cochlea.
Specimen 8B (top): postnatal day 1 (more )
Note: Double labeled: green - ZO-1 (Tjp1); magenta - Daple (Ccdc88c assay MGI:7427530).
Structure Level Pattern Image Note
TS27: cochlea Present Regionally restricted 8B (top) Expression at tight junctions.
Specimen 8B (bottom): postnatal day 1 (more )
Note: Double labeled: green - ZO-1 (Tjp1); magenta - Daple (Ccdc88c assay MGI:7427530).
Structure Level Pattern Image Note
TS27: cochlea Present Regionally restricted 8B (bottom) Expression at tight junctions.
Specimen 8C (top): postnatal day 1 (more )
Note: Double labeled: green - ZO-1 (Tjp1); magenta - Gnai3 (assay MGI:7427533).
Structure Level Pattern Image Note
TS27: cochlea Present Regionally restricted 8C (top) Expression at tight junctions.
Specimen 8C (bottom): postnatal day 1 (more )
Note: Double labeled: green - ZO-1 (Tjp1); magenta - Gnai3 (assay MGI:7427533).
Structure Level Pattern Image Note
TS27: cochlea Present Regionally restricted 8C (bottom) Expression at tight junctions.
Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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05/07/2024
MGI 6.23 		The Jackson Laboratory