Gene Expression Data
Assay Details
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Assay
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Reference:
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J:332131
Perera SN, et al., Insights into olfactory ensheathing cell development from a laser-microdissection and transcriptome-profiling approach. Glia. 2020 Dec;68(12):2550-2584
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Assay type:
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RNA in situ
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MGI Accession ID:
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MGI:7424993
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Gene symbol:
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Plp1
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Gene name:
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proteolipid protein (myelin) 1
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Probe:
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Plp1 cDNA1
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Probe preparation:
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Antisense labelled with digoxigenin RNA
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Assay notes:
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The probe used in this assay does not distinguish between isoforms.
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Results
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Specimen
6 - Plp:
embryonic day 16.5
(more  )
Note: Coronal section. Immunostained with Sox10 (green) to label olfactory entheathing cells (OECs) and Schwann cells and Tubb3 (magenta) to identify axons.
Specimen
6 - Plp:
(close  )
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Genetic Background:
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Not Specified
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Age:
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embryonic day 16.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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Note:
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Coronal section. Immunostained with Sox10 (green) to label olfactory entheathing cells (OECs) and Schwann cells and Tubb3 (magenta) to identify axons.
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Specimen
S8 - E11.5:
embryonic day 11.5
(more )
Note: Parasagittal section. Immunostained with Sox10 (green) to label olfactory entheathing cells (OECs) and Schwann cells and Tubb3 (magenta) to identify axons.
Specimen
S8 - E11.5:
(close )
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Genetic Background:
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Not Specified
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Age:
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embryonic day 11.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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Note:
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Parasagittal section. Immunostained with Sox10 (green) to label olfactory entheathing cells (OECs) and Schwann cells and Tubb3 (magenta) to identify axons.
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| Structure |
Cell Type |
Level |
Pattern |
Image |
Note |
| TS20: olfactory nerve |
olfactory ensheathing cell |
Weak |
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S8C
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S8B
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The mucosal OECs show expression.
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Specimen
S8 - E13 - D1:
embryonic day 13.0
(more )
Note: Coronal section. Immunostained with Sox10 (green) to label olfactory entheathing cells (OECs) and Schwann cells and Tubb3 (magenta) to identify axons.
Specimen
S8 - E13 - D1:
(close )
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Genetic Background:
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Not Specified
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Age:
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embryonic day 13.0
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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Note:
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Coronal section. Immunostained with Sox10 (green) to label olfactory entheathing cells (OECs) and Schwann cells and Tubb3 (magenta) to identify axons.
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Specimen
S8 - E13 - D2:
embryonic day 13.0
(more )
Note: Coronal section. Immunostained with Sox10 (green) to label olfactory entheathing cells (OECs) and Schwann cells and Tubb3 (magenta) to identify axons.
Specimen
S8 - E13 - D2:
(close )
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Genetic Background:
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Not Specified
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Age:
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embryonic day 13.0
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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Note:
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Coronal section. Immunostained with Sox10 (green) to label olfactory entheathing cells (OECs) and Schwann cells and Tubb3 (magenta) to identify axons.
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| Structure |
Cell Type |
Level |
Pattern |
Image |
Note |
| TS21: olfactory nerve |
olfactory ensheathing cell |
Present |
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S8D2
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S8E2
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The mucosal OECs show expression.
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Specimen
12a:
embryonic day 16.5
(more )
Note: Coronal section. Immunostained with Sox10 (green) to label olfactory entheathing cells (OECs) and Tubb3 (magenta) to identify axons.
Specimen
12a:
(close )
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Genetic Background:
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involves: 129S1/Sv * 129X1/SvJ
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Age:
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embryonic day 16.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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Note:
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Coronal section. Immunostained with Sox10 (green) to label olfactory entheathing cells (OECs) and Tubb3 (magenta) to identify axons.
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Specimen
12b:
embryonic day 16.5; Ptprz1tm1Mno/ Ptprz1tm1Mno
(more )
Note: Coronal section. Immunostained with Sox10 (green) to label olfactory entheathing cells (OECs) and Tubb3 (magenta) to identify axons.
Specimen
12b:
(close )
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Genetic Background:
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involves: 129S1/Sv * 129X1/SvJ
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Mutant Allele(s):
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Ptprz1tm1Mno/Ptprz1tm1Mno
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Age:
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embryonic day 16.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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Note:
|
Coronal section. Immunostained with Sox10 (green) to label olfactory entheathing cells (OECs) and Tubb3 (magenta) to identify axons.
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| Structure |
Cell Type |
Level |
Pattern |
Image |
Note |
| TS24: main olfactory bulb olfactory nerve layer |
olfactory ensheathing cell |
Present |
Non-Uniform |
12b1
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12b
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The inner ONL-OECs show stronger expression than the outer ones. Expression levels in both the inner and outer OECs of the mutant are higher than those seen in wild type.
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Specimen
12c:
embryonic day 16.5
(more )
Note: Coronal section. Immunostained with Sox10 (green) to label olfactory entheathing cells (OECs) and Tubb3 (magenta) to identify axons.
Specimen
12c:
(close )
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Genetic Background:
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involves: 129S1/Sv * 129X1/SvJ
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Age:
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embryonic day 16.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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|
Note:
|
Coronal section. Immunostained with Sox10 (green) to label olfactory entheathing cells (OECs) and Tubb3 (magenta) to identify axons.
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| Structure |
Cell Type |
Level |
Pattern |
Image |
Note |
| TS24: olfactory nerve |
olfactory ensheathing cell |
Present |
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12c
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12c1
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The mucosal OECs show expression.
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Specimen
12d:
embryonic day 16.5; Ptprz1tm1Mno/ Ptprz1tm1Mno
(more )
Note: Coronal section. Immunostained with Sox10 (green) to label olfactory entheathing cells (OECs) and Tubb3 (magenta) to identify axons.
Specimen
12d:
(close )
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Genetic Background:
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involves: 129S1/Sv * 129X1/SvJ
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Mutant Allele(s):
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Ptprz1tm1Mno/Ptprz1tm1Mno
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Age:
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embryonic day 16.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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|
Note:
|
Coronal section. Immunostained with Sox10 (green) to label olfactory entheathing cells (OECs) and Tubb3 (magenta) to identify axons.
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| Structure |
Cell Type |
Level |
Pattern |
Image |
Note |
| TS24: olfactory nerve |
olfactory ensheathing cell |
Present |
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12d
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12d1
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The mucosal OECs show expression. Expression levels in the mutant are higher than those seen in wild type.
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Specimen
12e:
embryonic day 16.5
(more )
Note: Coronal section. Immunostained with Sox10 (green) to label olfactory entheathing cells (OECs) and Tubb3 (magenta) to identify axons.
Specimen
12e:
(close )
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Genetic Background:
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involves: 129S1/Sv * 129X1/SvJ
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Age:
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embryonic day 16.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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Note:
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Coronal section. Immunostained with Sox10 (green) to label olfactory entheathing cells (OECs) and Tubb3 (magenta) to identify axons.
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Specimen
12f:
embryonic day 16.5; Ptprz1tm1Mno/ Ptprz1tm1Mno
(more )
Note: Coronal section. Immunostained with Sox10 (green) to label olfactory entheathing cells (OECs) and Tubb3 (magenta) to identify axons.
Specimen
12f:
(close )
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Genetic Background:
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involves: 129S1/Sv * 129X1/SvJ
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Mutant Allele(s):
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Ptprz1tm1Mno/Ptprz1tm1Mno
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Age:
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embryonic day 16.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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|
Note:
|
Coronal section. Immunostained with Sox10 (green) to label olfactory entheathing cells (OECs) and Tubb3 (magenta) to identify axons.
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| Structure |
Cell Type |
Level |
Pattern |
Image |
Note |
| TS24: trigeminal nerve |
Schwann cell |
Present |
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12f1
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12f
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The Schwann cells show expression. Expression levels in the mutant are comparable to those seen in wild type.
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Specimen
S14A:
embryonic day 16.5
(more )
Note: Coronal section. Immunostained with Sox10 (green) to label olfactory entheathing cells (OECs) and Tubb3 (magenta) to identify axons.
Specimen
S14A:
(close )
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Genetic Background:
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involves: 129S2/SvPas
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Age:
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embryonic day 16.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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Note:
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Coronal section. Immunostained with Sox10 (green) to label olfactory entheathing cells (OECs) and Tubb3 (magenta) to identify axons.
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Specimen
S14B:
embryonic day 16.5; Ptntm1Tmu/ Ptntm1Tmu
(more )
Note: Coronal section. Immunostained with Sox10 (green) to label olfactory entheathing cells (OECs) and Tubb3 (magenta) to identify axons.
Specimen
S14B:
(close )
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Genetic Background:
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involves: 129S2/SvPas
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Mutant Allele(s):
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Ptntm1Tmu/Ptntm1Tmu
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Age:
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embryonic day 16.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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|
Note:
|
Coronal section. Immunostained with Sox10 (green) to label olfactory entheathing cells (OECs) and Tubb3 (magenta) to identify axons.
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| Structure |
Cell Type |
Level |
Pattern |
Image |
Note |
| TS24: main olfactory bulb olfactory nerve layer |
olfactory ensheathing cell |
Very strong |
Non-Uniform |
S14B
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S14B1
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The inner ONL-OECs show stronger expression than the outer ones. Expression levels in the mutant are comparable to those seen in wild type.
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Specimen
S14C:
embryonic day 16.5
(more )
Note: Coronal section. Immunostained with Sox10 (green) to label olfactory entheathing cells (OECs) and Tubb3 (magenta) to identify axons.
Specimen
S14C:
(close )
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Genetic Background:
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involves: 129S2/SvPas
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Age:
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embryonic day 16.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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Note:
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Coronal section. Immunostained with Sox10 (green) to label olfactory entheathing cells (OECs) and Tubb3 (magenta) to identify axons.
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Specimen
S14D:
embryonic day 16.5; Ptntm1Tmu/ Ptntm1Tmu
(more )
Note: Coronal section. Immunostained with Sox10 (green) to label olfactory entheathing cells (OECs) and Tubb3 (magenta) to identify axons.
Specimen
S14D:
(close )
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Genetic Background:
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involves: 129S2/SvPas
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Mutant Allele(s):
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Ptntm1Tmu/Ptntm1Tmu
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Age:
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embryonic day 16.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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Note:
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Coronal section. Immunostained with Sox10 (green) to label olfactory entheathing cells (OECs) and Tubb3 (magenta) to identify axons.
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| Structure |
Cell Type |
Level |
Pattern |
Image |
Note |
| TS24: olfactory nerve |
olfactory ensheathing cell |
Present |
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S14D1
,
S14D
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The mucosal OECs show expression. Expression levels in the mutant are comparable to those seen in wild type.
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