Probe:	G90 cDNA
Probe preparation:	Antisense labelled with S35 RNA
Visualized with:	Autoradiography
Assay notes:	Fix: Serra's (60% ethanol, 30% formalin, 10% acetic acid).

Structure	Level	Pattern	Image	Note
TS15: midbrain-hindbrain junction	Present	Regionally restricted		Expression was restricted to the ventricular zone of the isthmus between the third and fourth ventricles.
TS15: otocyst	Present	Regionally restricted		Expression was detected in the ventro-medial wall of the otic vesicle.
TS15: olfactory placode	Present

Structure	Level	Pattern	Image	Note
TS17: midbrain-hindbrain junction	Present			Expression was similar to E9.5.
TS17: olfactory placode	Present			Expression was similar to E9.5.
TS17: otocyst	Present			Expression was similar to E9.5.

Structure	Level	Pattern	Image	Note
TS19: telencephalon	Present	Regionally restricted	1A/B	Expression was detected in the wall of the telencephalic vesicle.
TS19: otocyst	Present	Regionally restricted	1A/B	Expression was detected in the ventro-medial wall of the otic vesicle.

Structure	Level	Pattern	Image	Note
TS19: midbrain-hindbrain junction	Present	Regionally restricted	1C/D	Expression was detected in the ventricular zone of the isthmus between the third and fourth ventricles.

Structure	Level	Pattern	Image	Note
TS19: otocyst	Present	Regionally restricted	1E/F	Expression was detected in the ventro-medial wall of the otic vesicle.

Structure	Level	Pattern	Image	Note
TS19: nasal pit	Present	Regionally restricted	1G/H	Expression was detected in the dorsal wall of the olfactory pit.

Structure	Level	Pattern	Image	Note
TS19: tongue epithelium	Present	Regionally restricted	1I/J	Expression was detected in the median circumvallate papilla located at the back of the tongue.

Structure	Level	Pattern	Image	Note
TS22: hippocampus	Present		1K/L

Structure	Level	Pattern	Image	Note
TS21: oropharynx	Present	Regionally restricted		Expression was detected in oropharyngeal epithelia.
TS21: tongue epithelium	Present	Regionally restricted		Expression was detected in the median circumvallate papilla of the tongue.

Structure	Level	Pattern	Image	Note
TS23: epiglottis	Present

Structure	Level	Pattern	Image	Note
TS25: epiglottis	Present
TS25: oropharynx	Present	Regionally restricted		Expression was detected in oropharyngeal epithelia.
TS25: circumvallate papillae	Present	Regionally restricted		Expression was detected in the median circumvallate papilla of the tongue.

Structure	Level	Pattern	Image	Note
TS21: cochlea	Present		2A/B
TS21: cochlear ganglion	Present		2A/B
TS21: pharyngo-tympanic tube	Present		2A/B

Structure	Cell Type	Level	Pattern	Image	Note
TS21: semicircular canal		Present	Regionally restricted	2C/D	Expression was detected in the crista of the ampullae.
TS21: utricle	sensory hair cell	Present	Regionally restricted	2C/D	Expression was detected in the macula of the utriculus.
TS21: utricle	supporting cell	Present	Regionally restricted	2C/D	Expression was detected in the macula of the utriculus.
TS21: vestibular ganglion		Present		2C/D

Structure	Level	Pattern	Image	Note
TS25: cochlea	Present		2E/F
TS25: cochlear ganglion	Present		2E/F
TS25: tubotympanic recess	Present		2E/F

Structure	Cell Type	Level	Pattern	Image	Note
TS25: vestibular ganglion		Present		2G/H
TS25: macula of saccule	sensory hair cell	Present		2G/H
TS25: macula of saccule	supporting cell	Present		2G/H
TS25: macula of utricle	sensory hair cell	Present		2G/H
TS25: macula of utricle	supporting cell	Present		2G/H
TS25: semicircular canal	sensory hair cell	Present	Regionally restricted	2G/H	Expression was detected in the crista of the ampullae.
TS25: semicircular canal	supporting cell	Present	Regionally restricted	2G/H	Expression was detected in the crista of the ampullae.

Structure	Cell Type	Level	Pattern	Image	Note
TS23: cochlea	sensory hair cell	Present	Regionally restricted	2J	Expression was detected in sensory hair cells of the organ of Corti.
TS23: cochlea	organ of Corti supporting cell	Present	Regionally restricted	2J	Expression was detected in a subset of supporting cells of the organ of Corti.
TS23: greater epithelial ridge		Present	Widespread	2J	Expression was detected in most cells of the greater epithelial ridge.

Structure	Cell Type	Level	Pattern	Image	Note
TS25: organ of Corti	sensory hair cell	Absent		2M
TS25: organ of Corti	organ of Corti supporting cell	Present	Regionally restricted	2M	Expression was detected in a subset of supporting cells of the organ of Corti.
TS25: greater epithelial ridge		Present	Widespread	2M	Expression was detected in most cells of the greater epithelial ridge.

Structure	Cell Type	Level	Pattern	Image	Note
TS23: inner ear vestibular component	sensory hair cell	Present		2P
TS23: inner ear vestibular component	supporting cell of vestibular epithelium	Present		2P

Structure	Cell Type	Level	Pattern	Image	Note
TS25: inner ear vestibular component	sensory hair cell	Present		2R
TS25: inner ear vestibular component	supporting cell of vestibular epithelium	Present		2R

Structure	Level	Pattern	Image	Note
TS25: cochlea	Present		2S/T
TS25: cochlear ganglion	Present		2S/T

Structure	Level	Pattern	Image	Note
TS25: cochlea	Present		2U/V	The mutant expression pattern and level were comparable to wild type.
TS25: cochlear ganglion	Present		2U/V	The mutant expression pattern and level were comparable to wild type.

Structure	Cell Type	Level	Pattern	Image	Note
TS25: inner ear vestibular component		Present
TS25: organ of Corti	sensory hair cell	Absent

Structure	Cell Type	Level	Pattern	Image	Note
TS25: inner ear vestibular component		Present			Expression was normal in the mutant compared to a wild type littermate.
TS25: organ of Corti	sensory hair cell	Absent			Expression was normal in the mutant compared to a wild type littermate.

Structure	Level	Pattern	Image	Note
TS25: cochlea	Present	Regionally restricted	3A/B	Expression was detected in post-mitotic cells.
TS25: cochlear ganglion	Present	Regionally restricted	3A/B	Expression was detected in post-mitotic cells.

Structure	Level	Pattern	Image	Note
TS23: oral cavity	Present	Regionally restricted	3D/E	Expression was detected in the papillae along the oral cavity. Expression was detected in post-mitotic cells.

Structure	Level	Pattern	Image	Note
TS23: olfactory epithelium	Present		3G/H	Expression was detected in post-mitotic cells.

Structure	Level	Pattern	Image	Note
TS23: vomeronasal organ	Present		3J/K	Expression was detected in post-mitotic cells.

Structure	Level	Pattern	Image	Note
TS25: nasopharynx epithelium	Present	Regionally restricted	3M/N	Expression was detected in post-mitotic epithelial cells.

Structure	Level	Pattern	Image	Note
TS23: pituitary gland	Present		3P/Q	A partial overlap of expression was detected with the histone H3 proliferation marker.

Structure	Level	Pattern	Image	Note
TS19: telencephalon	Present	Regionally restricted	3S/T	Expression was detected in the proliferating zones of the telencephalic vesicle.

Structure	Level	Pattern	Image	Note
TS27: cochlea	Present
TS27: inner ear vestibular component	Present
TS27: olfactory epithelium	Present
TS27: vomeronasal organ	Present

Structure	Level	Pattern	Image	Note
TS28: inner ear vestibular component	Present