Probe:	Ptch probe10
Probe preparation:	labelled with S35

Structure	Level	Pattern	Image	Note
TS22: mesenchyme of metanephros	Strong	Regionally restricted	1C	High levels of expression in most layers of mesenchymal cells adjacent to epithelium of collecting ducts and ureter.
TS22: ureteral mesenchyme	Strong	Regionally restricted	1C	High levels of expression in most layers of mesenchymal cells adjacent to epithelium of collecting ducts and ureter.

Structure	Level	Pattern	Image	Note
TS27: renal medulla	Present	Regionally restricted	1G inset , 1G	Expression in mesenchyme of medulla and renal pelvis.
TS27: renal pelvis	Present	Regionally restricted	1G inset , 1G	Expression in mesenchyme of medulla and renal pelvis.

Structure	Cell Type	Level	Pattern	Image	Note
TS27: ureter		Present	Regionally restricted	1K	Expression restricted to think layer of mesenchymal cells immediately adjacent to epithelium.
TS27: ureteral smooth muscle layer smooth muscle component	ureter smooth muscle cell	Absent		1K	No expression in differentiated ureter smooth muscle cells.

Structure	Level	Pattern	Image	Note
TS22: metanephros	Present		4A

Structure	Level	Pattern	Image	Note
TS22: metanephros	Absent		4B	Expression reduced to basal levels.

Structure	Level	Pattern	Image	Note
TS22: ureter	Present		4C

Structure	Level	Pattern	Image	Note
TS22: ureter	Absent		4D	Expression reduced to basal levels.