Pou5f1 Immunohistochemistry Gene Expression Assay

Gene Expression Data
Assay Details

Assay

Reference:	J:329966 Bell CJ, et al., Borcs6 is required for endo-lysosomal degradation during early development. Mol Reprod Dev. 2022 Aug;89(8):337-350
Assay type:	Immunohistochemistry
MGI Accession ID:	MGI:7398484
Gene symbol:	Pou5f1
Gene name:	POU domain, class 5, transcription factor 1

Antibody:	anti-Oct4 (ab27985)
Detection system:	Secondary antibody coupled to Alexa Fluor

Results

2j ©

2j'''' ©

Specimen 2j/j'''': embryonic day 6.5 (more

)
Note: Triple labeled: white - Oct4 (Pou5f1); green - E-cadherin (Cdh1 assay MGI:7398481); red - Cdx2 (assay MGI:7398483).

Structure	Level	Pattern	Image	Note
TS9: embryo ectoderm	Present		2j'''' , 2j	Expression in epiblast.

2k ©

2k'''' ©

Specimen 2k/k'''': embryonic day 6.5; Borcs6^{tm1.1(KOMP)Vlcg}/Borcs6^{tm1.1(KOMP)Vlcg} (more

)
Note: Triple labeled: white - Oct4 (Pou5f1); green - E-cadherin (Cdh1 assay MGI:7398481); red - Cdx2 (assay MGI:7398483).

Structure	Level	Pattern	Image	Note
TS9: embryo ectoderm	Present		2k'''' , 2k	Epiblast appeared much smaller with fewer expressing cells.

3a ©

3c'' ©

Specimen 3a/c'': embryonic day 6.5 (more

)
Note: Triple labeled: white - Oct4 (Pou5f1); green - E-cadherin (Cdh1 assay MGI:7398481); red - Cdx2 (assay MGI:7398483).

Structure	Level	Pattern	Image	Note
TS9: embryo ectoderm	Present		3a , 3c''	Expression in epiblast.

3b ©

3d'' ©

Specimen 3b/d'': embryonic day 6.5; Borcs6^{tm1.1(KOMP)Vlcg}/Borcs6⁺ (more

)
Note: Triple labeled: white - Oct4 (Pou5f1); green - E-cadherin (Cdh1 assay MGI:7398481); red - Cdx2 (assay MGI:7398483).

Structure	Level	Pattern	Image	Note
TS9: embryo ectoderm	Present		3b , 3d''	Epiblast appeared much smaller with fewer expressing cells.

Contributing Projects: Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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