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Gene Expression Data
Assay Details
Assay
Reference: J:329966 Bell CJ, et al., Borcs6 is required for endo-lysosomal degradation during early development. Mol Reprod Dev. 2022 Aug;89(8):337-350
Assay type: Immunohistochemistry
MGI Accession ID: MGI:7398481
Gene symbol: Cdh1
Gene name: cadherin 1
Antibody: Anti-E-Cadherin (610181; Clone 36)
Detection system: Secondary antibody coupled to Alexa Fluor
Results
Specimen 2b: embryonic day 7.5 (more )
Note: Double labeled: red - E-cadherin (Cdh1); green - Brachyury (T assay MGI:7398482).
Structure Level Pattern Image Note
TS11: embryo ectoderm Present 2b
TS11: extraembryonic ectoderm Present 2b
Specimen 2c: embryonic day 7.5; Borcs6tm1.1(KOMP)Vlcg/Borcs6tm1.1(KOMP)Vlcg (more )
Note: Double labeled: red - E-cadherin (Cdh1); green - Brachyury (T assay MGI:7398482).
Structure Level Pattern Image Note
TS11: ectoderm Present 2c Authors state that epiblast and extraembryonic ectoderm appear to have died.
Specimen 2j/j'': embryonic day 6.5 (more )
Note: Triple labeled: green - E-cadherin (Cdh1); red - Cdx2 (assay MGI:7398483); white - Oct4 (Pou5f1 assay MGI:7398484).
Structure Level Pattern Image Note
TS9: embryo ectoderm Present Regionally restricted 2j'' , 2j Embryos composed of organized cells with uniformly sized nuclei.
TS9: extraembryonic ectoderm Present Regionally restricted 2j'' , 2j Embryos composed of organized cells with uniformly sized nuclei.
Specimen 2k/k'': embryonic day 6.5; Borcs6tm1.1(KOMP)Vlcg/Borcs6tm1.1(KOMP)Vlcg (more )
Note: Triple labeled: green - E-cadherin (Cdh1); red - Cdx2 (assay MGI:7398483); white - Oct4 (Pou5f1 assay MGI:7398484).
Structure Level Pattern Image Note
TS9: ectoderm Present Regionally restricted 2k , 2k'' Authors describe cells with larger nuclei and lacking epithelial organization.
Specimen 3a/c: embryonic day 6.5 (more )
Note: Triple labeled: green - E-cadherin (Cdh1); red - Cdx2 (assay MGI:7398483); white - Oct4 (Pou5f1 assay MGI:7398484).
Structure Level Pattern Image Note
TS9: extraembryonic ectoderm Present 3c , 3a , 3c'
TS9: embryo ectoderm Present 3a , 3c'' , 3c
Specimen 3b/d: embryonic day 6.5; Borcs6tm1.1(KOMP)Vlcg/Borcs6+ (more )
Note: Triple labeled: green - E-cadherin (Cdh1); red - Cdx2 (assay MGI:7398483); white - Oct4 (Pou5f1 assay MGI:7398484).
Structure Level Pattern Image Note
TS9: ectoderm Present 3d' , 3d'' , 3d , 3b
Specimen 4a/c: embryonic day 6.5 (more )
Note: Triple labeled: green - E-cadherin (Cdh1); red - Rab7 (assay MGI:7398485); white - Lamp1 (assay MGI:7398486).
Structure Level Pattern Image Note
TS9: embryo ectoderm Present 4a , 4c
TS9: extraembryonic ectoderm Present 4a , 4c
Specimen 4b/d: embryonic day 6.5 (more )
Note: Triple labeled: green - E-cadherin (Cdh1); red - Rab7 (assay MGI:7398485); white - Lamp1 (assay MGI:7398486).
Structure Level Pattern Image Note
TS9: ectoderm Present 4d , 4b
Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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05/07/2024
MGI 6.23 		The Jackson Laboratory