Pou5f1 Immunohistochemistry Gene Expression Assay

Gene Expression Data
Assay Details

Assay

Reference:	J:329966 Bell CJ, et al., Borcs6 is required for endo-lysosomal degradation during early development. Mol Reprod Dev. 2022 Aug;89(8):337-350
Assay type:	Immunohistochemistry
MGI Accession ID:	MGI:7398484
Gene symbol:	Pou5f1
Gene name:	POU domain, class 5, transcription factor 1

Antibody:	anti-Oct4 (ab27985)
Detection system:	Secondary antibody coupled to Alexa Fluor

Results

2j ©

2j'''' ©

Specimen 2j/j'''': embryonic day 6.5 (more

)
Note: Triple labeled: white - Oct4 (Pou5f1); green - E-cadherin (Cdh1 assay MGI:7398481); red - Cdx2 (assay MGI:7398483).

Structure	Level	Pattern	Image	Note
TS9: embryo ectoderm	Present		2j'''' , 2j	Expression in epiblast.

2k ©

2k'''' ©

Specimen 2k/k'''': embryonic day 6.5; Borcs6^{tm1.1(KOMP)Vlcg}/Borcs6^{tm1.1(KOMP)Vlcg} (more

)
Note: Triple labeled: white - Oct4 (Pou5f1); green - E-cadherin (Cdh1 assay MGI:7398481); red - Cdx2 (assay MGI:7398483).

Structure	Level	Pattern	Image	Note
TS9: embryo ectoderm	Present		2k'''' , 2k	Epiblast appeared much smaller with fewer expressing cells.

3a ©

3c'' ©

Specimen 3a/c'': embryonic day 6.5 (more

)
Note: Triple labeled: white - Oct4 (Pou5f1); green - E-cadherin (Cdh1 assay MGI:7398481); red - Cdx2 (assay MGI:7398483).

Structure	Level	Pattern	Image	Note
TS9: embryo ectoderm	Present		3a , 3c''	Expression in epiblast.

3b ©

3d'' ©

Specimen 3b/d'': embryonic day 6.5; Borcs6^{tm1.1(KOMP)Vlcg}/Borcs6⁺ (more

)
Note: Triple labeled: white - Oct4 (Pou5f1); green - E-cadherin (Cdh1 assay MGI:7398481); red - Cdx2 (assay MGI:7398483).

Structure	Level	Pattern	Image	Note
TS9: embryo ectoderm	Present		3b , 3d''	Epiblast appeared much smaller with fewer expressing cells.

Contributing Projects: Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
Citing These Resources Funding Information Warranty Disclaimer, Privacy Notice, Licensing, & Copyright Send questions and comments to User Support.	last database update 05/21/2024 MGI 6.23