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Gene Expression Data
Assay Details
Assay
Reference: J:314181 Holzman MA, et al., HOXA5 Participates in Brown Adipose Tissue and Epaxial Skeletal Muscle Patterning and in Brown Adipocyte Differentiation. Front Cell Dev Biol. 2021;9:632303
Assay type: Immunohistochemistry
MGI Accession ID: MGI:7384940
Gene symbol: Acta1
Gene name: actin alpha 1, skeletal muscle
Antibody: anti-muscle actin (ab156302)
Detection system: Secondary antibody
Results
Specimen 4C: embryonic day 18.5 (more )
Note: Double labeled: red - Acta1; green - Pdgfra (assay MGI:7384939).
Structure Level Pattern Image Note
TS26: skeletal muscle Present Regionally restricted 4C Expression in rhomboid muscle.
Specimen 4D: embryonic day 18.5 (more )
Note: Double labeled: red - Acta1; green - Pdgfra (assay MGI:7384939).
Structure Level Pattern Image Note
TS26: skeletal muscle Present Regionally restricted 4D Expression in splenius muscle.
Specimen 4E: embryonic day 18.5 (more )
Note: Double labeled: red - Acta1; green - Pdgfra (assay MGI:7384939).
Structure Level Pattern Image Note
TS26: skeletal muscle Present Regionally restricted 4E Expression in prevertebral muscles.
Specimen 4F: embryonic day 18.5 (more )
Note: Double labeled: red - Acta1; green - Pdgfra (assay MGI:7384939).
Structure Level Pattern Image Note
TS26: skeletal muscle Present Regionally restricted 4F Expression in prevertebral muscles.
Specimen 4G: embryonic day 18.5; Hoxa5tm1Rob/Hoxa5tm1Rob (more )
Note: Double labeled: red - Acta1; green - Pdgfra (assay MGI:7384939).
Structure Level Pattern Image Note
TS26: skeletal muscle Present Regionally restricted 4G Expansion in area occupied by epaxial muscles (rhomboid, splenius and semispinalis muscles ) developing interleaved between brown adipose tissue (BAT) deposits.
Specimen 4H: embryonic day 18.5; Hoxa5tm1Rob/Hoxa5tm1Rob (more )
Note: Double labeled: red - Acta1; green - Pdgfra (assay MGI:7384939).
Structure Level Pattern Image Note
TS26: skeletal muscle Present Regionally restricted 4H Expansion in area occupied by epaxial muscles (rhomboid, splenius and semispinalis muscles ) developing interleaved between brown adipose tissue (BAT) deposits.
Specimen 4I: embryonic day 18.5; Hoxa5tm1Rob/Hoxa5tm1Rob (more )
Note: Double labeled: red - Acta1; green - Pdgfra (assay MGI:7384939).
Structure Level Pattern Image Note
TS26: skeletal muscle Present Regionally restricted 4I Reduction in area occupied by several hypaxial muscles (prevertebral (longus), sternothyroid and intercostal muscles between T1 and T2).
Specimen 4J: embryonic day 18.5; Hoxa5tm1Rob/Hoxa5tm1Rob (more )
Note: Double labeled: red - Acta1; green - Pdgfra (assay MGI:7384939).
Structure Level Pattern Image Note
TS26: skeletal muscle Present Regionally restricted 4J Reduction in area occupied by several hypaxial muscles (prevertebral (longus), sternothyroid and intercostal muscles between T1 and T2).
Specimen 7D: embryonic day 18.5; Hoxa5tm1.1Ljea/Hoxa5+, Myf5tm3(cre)Sor/Myf5+ (more )
Note: Conditional mutant. Double labeled: red - Acta1; green - Pdgfra (assay MGI:7384939).
Structure Level Pattern Image Note
TS26: skeletal muscle Present Regionally restricted 7D Expression in rhomboid and splenius muscles.
Specimen 7E: embryonic day 18.5; Hoxa5tm1.1Ljea/Hoxa5+, Myf5tm3(cre)Sor/Myf5+ (more )
Note: Conditional mutant. Double labeled: red - Acta1; green - Pdgfra (assay MGI:7384939).
Structure Level Pattern Image Note
TS26: skeletal muscle Present Regionally restricted 7E Expression in rhomboid and splenius muscles.
Specimen 7F: embryonic day 18.5; Hoxa5tm1.1Ljea/Hoxa5+, Myf5tm3(cre)Sor/Myf5+ (more )
Note: Conditional mutant. Double labeled: red - Acta1; green - Pdgfra (assay MGI:7384939).
Structure Level Pattern Image Note
TS26: skeletal muscle Present Regionally restricted 7F Expression in prevertebral muscles.
Specimen 7G: embryonic day 18.5; Hoxa5tm1.1Ljea/Hoxa5+, Myf5tm3(cre)Sor/Myf5+ (more )
Note: Conditional mutant. Double labeled: red - Acta1; green - Pdgfra (assay MGI:7384939).
Structure Level Pattern Image Note
TS26: skeletal muscle Present Regionally restricted 7G Expression in sternothyroid and first intercostal muscles.
Specimen 7H: embryonic day 18.5; Hoxa5tm1.1Ljea/Hoxa5tm1.1Ljea, Myf5tm3(cre)Sor/Myf5+ (more )
Note: Conditional mutant. Double labeled: red - Acta1; green - Pdgfra (assay MGI:7384939).
Structure Level Pattern Image Note
TS26: skeletal muscle Present Regionally restricted 7H Expression somewhat expanded in epaxial (rhomboid and splenius) muscles, less severe than in Hoxa5 null embryos.
Specimen 7I: embryonic day 18.5; Hoxa5tm1.1Ljea/Hoxa5tm1.1Ljea, Myf5tm3(cre)Sor/Myf5+ (more )
Note: Conditional mutant. Double labeled: red - Acta1; green - Pdgfra (assay MGI:7384939).
Structure Level Pattern Image Note
TS26: skeletal muscle Present Regionally restricted 7I Expression somewhat expanded in epaxial (rhomboid and splenius) muscles, less severe than in Hoxa5 null embryos.
Specimen 7J: embryonic day 18.5; Hoxa5tm1.1Ljea/Hoxa5tm1.1Ljea, Myf5tm3(cre)Sor/Myf5+ (more )
Note: Conditional mutant. Double labeled: red - Acta1; green - Pdgfra (assay MGI:7384939).
Structure Level Pattern Image Note
TS26: skeletal muscle Present Regionally restricted 7J Expression not reduced in hypaxial (prevertebral and first intercostal) muscles.
Specimen 7K: embryonic day 18.5; Hoxa5tm1.1Ljea/Hoxa5tm1.1Ljea, Myf5tm3(cre)Sor/Myf5+ (more )
Note: Conditional mutant. Double labeled: red - Acta1; green - Pdgfra (assay MGI:7384939).
Structure Level Pattern Image Note
TS26: skeletal muscle Present Regionally restricted 7K Expression not reduced in hypaxial (prevertebral and first intercostal) muscles.
Specimen 8F: embryonic day 18.5; Gt(ROSA)26Sortm1.1(EYFP)Cos/Gt(ROSA)26Sor+, Hoxa5tm1Rob/Hoxa5+, Tg(Hoxa5-cre)447BLjea/0 (more )
Note: Conditional mutant. Double labeled: red - Acta1; green - Cre reporter.
Structure Level Pattern Image Note
TS26: skeletal muscle Present Regionally restricted 8F
Specimen 8G: embryonic day 18.5; Gt(ROSA)26Sortm1.1(EYFP)Cos/Gt(ROSA)26Sor+, Hoxa5tm1Rob/Hoxa5+, Tg(Hoxa5-cre)447BLjea/0 (more )
Note: Conditional mutant. Double labeled: red - Acta1; green - Cre reporter.
Structure Level Pattern Image Note
TS26: skeletal muscle Present Regionally restricted 8G
Specimen 8H: embryonic day 18.5; Gt(ROSA)26Sortm1.1(EYFP)Cos/Gt(ROSA)26Sor+, Hoxa5tm1Rob/Hoxa5tm1Rob, Tg(Hoxa5-cre)447BLjea/0 (more )
Note: Conditional mutant. Double labeled: red - Acta1; green - Cre reporter.
Structure Level Pattern Image Note
TS26: skeletal muscle Present Regionally restricted 8H
Specimen 8I: embryonic day 18.5; Gt(ROSA)26Sortm1.1(EYFP)Cos/Gt(ROSA)26Sor+, Hoxa5tm1Rob/Hoxa5tm1Rob, Tg(Hoxa5-cre)447BLjea/0 (more )
Note: Conditional mutant. Double labeled: red - Acta1; green - Cre reporter.
Structure Level Pattern Image Note
TS26: skeletal muscle Present Regionally restricted 8I
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03/25/2025
MGI 6.24 		The Jackson Laboratory