Reporter:	GFP
Antibody:	anti-GFP (600-106-215)
Detection system:	Secondary antibody coupled to Alexa Fluor 488

Structure	Cell Type	Level	Pattern	Image	Note
TS28: cerebral cortex	perivascular macrophage	Present	Regionally restricted	1b	Expression was detected in perivascular macrophages, identified based on their close proximity to the abluminal side of Pecam1+ endothelial cells between the endothelial and the parenchymal basement membrane (laminin+), the perivascular space.

Structure	Cell Type	Level	Pattern	Image	Note
TS28: brain meninges	meningeal macrophage	Present	Regionally restricted	1c	Expression was detected in subdural meninges, localized by their close proximity to fibroblasts expressing ER-TR7 (fibroblast antibody).

Structure	Cell Type	Level	Pattern	Image	Note
TS28: brain meninges	meningeal macrophage	Present		1h left	Expression was detected in meningeal macrophages co-expressing Ptprc.
TS28: brain	microglial cell	Present		1h left	Microglia expressed only Cx3cr1 reporter.

Structure	Cell Type	Level	Pattern	Image	Note
TS28: brain	perivascular macrophage	Present	Regionally restricted	1h center	Expression was detected in perivascular macrophages co-expressing Ptprc.
TS28: brain	microglial cell	Present		1h center	Microglia expressed only Cx3cr1 reporter.

Structure	Cell Type	Level	Pattern	Image	Note
TS28: choroid plexus epithelium	choroid plexus macrophage	Present	Regionally restricted	1h right	Expression was detected in choroid plexus macrophages co-expressing Ptprc.
TS28: choroid plexus stroma	choroid plexus macrophage	Present	Regionally restricted	1h right	Expression was detected in choroid plexus macrophages co-expressing Ptprc.

Structure	Cell Type	Level	Pattern	Image	Note
TS28: brain	perivascular macrophage	Present	Regionally restricted	1i	Expression was detected in perivascular macrophages co-expressing Mrc1.

Structure	Cell Type	Level	Pattern	Image	Note
TS15: head mesenchyme	meningeal macrophage	Present	Regionally restricted	2_E9.5	Expression was detected in macrophages in the subdural meninges.

Structure	Cell Type	Level	Pattern	Image	Note
TS20: meninges	meningeal macrophage	Present		2_E12.5 Meninges

Structure	Cell Type	Level	Pattern	Image	Note
TS21: meninges	meningeal macrophage	Present		2_E13.5 Meninges

Structure	Cell Type	Level	Pattern	Image	Note
TS24: meninges	meningeal macrophage	Present		2_E16.5 Meninges

Structure	Cell Type	Level	Pattern	Image	Note
TS28: meninges	meningeal macrophage	Present		2_P60 Meninges

Structure	Cell Type	Level	Pattern	Image	Note
TS20: brain	perivascular macrophage	Present		2_E12.5 Perivascular space

Structure	Cell Type	Level	Pattern	Image	Note
TS21: brain	perivascular macrophage	Present		2_E13.5 Perivascular space

Structure	Cell Type	Level	Pattern	Image	Note
TS24: brain	perivascular macrophage	Present		2_E16.5 Perivascular space

Structure	Cell Type	Level	Pattern	Image	Note
TS28: brain	perivascular macrophage	Present		2_P60 Perivascular space

Structure	Cell Type	Level	Pattern	Image	Note
TS20: choroid plexus epithelium	choroid plexus macrophage	Present		2_E12.5 Choroid plexus
TS20: choroid plexus stroma	choroid plexus macrophage	Present		2_E12.5 Choroid plexus

Structure	Cell Type	Level	Pattern	Image	Note
TS21: choroid plexus epithelium	choroid plexus macrophage	Present		2_E13.5 Choroid plexus
TS21: choroid plexus stroma	choroid plexus macrophage	Present		2_E13.5 Choroid plexus

Structure	Cell Type	Level	Pattern	Image	Note
TS24: choroid plexus epithelium	choroid plexus macrophage	Present		2_E16.5 Choroid plexus
TS24: choroid plexus stroma	choroid plexus macrophage	Present		2_E16.5 Choroid plexus

Structure	Cell Type	Level	Pattern	Image	Note
TS28: choroid plexus epithelium	choroid plexus macrophage	Present		2_P60 Choroid plexus
TS28: choroid plexus stroma	choroid plexus macrophage	Present		2_P60 Choroid plexus

Structure	Cell Type	Level	Pattern	Image	Note
TS22: meninges	meningeal macrophage	Present		3f Meninges Cx3cr1GFP/WT	GFP and Aif1 expression co-localized in meningeal macrophages.

Structure	Cell Type	Level	Pattern	Image	Note
TS22: choroid plexus epithelium	choroid plexus macrophage	Present		3f Choriod plexus Cx3cr1GFP/WT	GFP and Aif1 expression co-localized in choroid plexus macrophages.
TS22: choroid plexus stroma	choroid plexus macrophage	Present		3f Choriod plexus Cx3cr1GFP/WT	GFP and Aif1 expression co-localized in choroid plexus macrophages.

Structure	Cell Type	Level	Pattern	Image	Note
TS22: meninges	meningeal macrophage	Present		3f Meninges Irf8-/- Cx3cr1GFP/WT	There was a reduction in the number of meningeal macrophages noted in the Irf8 mutant background.

Structure	Cell Type	Level	Pattern	Image	Note
TS22: choroid plexus epithelium	choroid plexus macrophage	Present		3f Choriod plexus Irf8-/- Cx3cr1GFP/WT	Choroid plexus macrophages were not affected in the Irf8 mutant background.
TS22: choroid plexus stroma	choroid plexus macrophage	Present		3f Choriod plexus Irf8-/- Cx3cr1GFP/WT	Choroid plexus macrophages were not affected in the Irf8 mutant background.