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Gene Expression Data
Assay Details
Assay
Reference: J:322165 Cunningham JG, et al., Early construction of the thalamocortical axon pathway requires c-Jun N-terminal kinase signaling within the ventral forebrain. Dev Dyn. 2022 Mar;251(3):459-480
Assay type: Immunohistochemistry
MGI Accession ID: MGI:7382852
Gene symbol: Isl1
Gene name: ISL1 transcription factor, LIM/homeodomain
Antibody: Human Islet-1 Antibody (AF1837)
Detection system: Secondary antibody coupled to Alexa Fluor 647
Results
Specimen 6B: embryonic day 13.5; Tg(mI56i-cre,EGFP)1Kc/0 (more )
Note: Double labeled: red - Isl1; green - Nkx2-1 (assay MGI:7382853).
Structure Level Pattern Image Note
TS21: forebrain Present Regionally restricted 6B Positive cells formed a permissive corridor between the Nkx2-1 positive medial ganglionic eminence and globus pallidus through which unlabeled thalamocortical axons extended.
Specimen 6C: embryonic day 13.5; Tg(mI56i-cre,EGFP)1Kc/0 (more )
Note: Double labeled: red - Isl1; green - Nkx2-1 (assay MGI:7382853).
Structure Level Pattern Image Note
TS21: forebrain Present Regionally restricted 6C Positive cells formed a permissive corridor between the Nkx2-1 positive medial ganglionic eminence and globus pallidus through which unlabeled thalamocortical axons extended.
Specimen 6D: embryonic day 13.5; Tg(mI56i-cre,EGFP)1Kc/0 (more )
Note: Double labeled: red - Isl1; green - Nkx2-1 (assay MGI:7382853).
Structure Level Pattern Image Note
TS21: forebrain Present Regionally restricted 6D Positive cells formed a permissive corridor between the Nkx2-1 positive medial ganglionic eminence and globus pallidus through which unlabeled thalamocortical axons extended.
TS21: telencephalon Present 6D
Specimen 6E: embryonic day 13.5; Tg(mI56i-cre,EGFP)1Kc/0 (more )
Note: Double labeled: red - Isl1; green - Nkx2-1 (assay MGI:7382853).
Structure Level Pattern Image Note
TS21: forebrain Present Regionally restricted 6E Thalamocortical axons (asterisk, unlabeled) crossed the diencephalon-telencephalon boundary above a patch of Nkx2-1 positive cells and Isl1 positive cells from the diencephalon.
TS21: diencephalon Present 6E
TS21: telencephalon Present 6E
Specimen 6F: embryonic day 13.5; Mapk10tm1Flv/Mapk10tm1Flv, Mapk8tm1Rjd/Mapk8tm1Rjd, Mapk9tm1Flv/Mapk9tm1Flv, Tg(mI56i-cre,EGFP)1Kc/0 (more )
Note: Conditional mutant. Double labeled: red - Isl1; green - Nkx2-1 (assay MGI:7382853).
Structure Level Pattern Image Note
TS21: forebrain Present 6F The positive corridor was present at rostral levels, similar as in the control.
Specimen 6G: embryonic day 13.5; Mapk10tm1Flv/Mapk10tm1Flv, Mapk8tm1Rjd/Mapk8tm1Rjd, Mapk9tm1Flv/Mapk9tm1Flv, Tg(mI56i-cre,EGFP)1Kc/0 (more )
Note: Conditional mutant. Double labeled: red - Isl1; green - Nkx2-1 (assay MGI:7382853).
Structure Level Pattern Image Note
TS21: forebrain Present Regionally restricted 6G The positive corridor was ectopically positioned laterally and ventrally compared with the control.
Specimen 6H: embryonic day 13.5; Mapk10tm1Flv/Mapk10tm1Flv, Mapk8tm1Rjd/Mapk8tm1Rjd, Mapk9tm1Flv/Mapk9tm1Flv, Tg(mI56i-cre,EGFP)1Kc/0 (more )
Note: Conditional mutant. Double labeled: red - Isl1; green - Nkx2-1 (assay MGI:7382853).
Structure Level Pattern Image Note
TS21: forebrain Present 6H The positive corridor was severely reduced at caudal levels, compared with the control, and no thalamocortical axons had crossed the diencephalon-telencephalon boundary.
Specimen 6I: embryonic day 13.5; Mapk10tm1Flv/Mapk10tm1Flv, Mapk8tm1Rjd/Mapk8tm1Rjd, Mapk9tm1Flv/Mapk9tm1Flv, Tg(mI56i-cre,EGFP)1Kc/0 (more )
Note: Conditional mutant. Double labeled: red - Isl1; green - Nkx2-1 (assay MGI:7382853).
Structure Level Pattern Image Note
TS21: forebrain Present Regionally restricted 6I The positive corridor was severely reduced at caudal levels, compared with the control, and no thalamocortical axons had crossed the diencephalon-telencephalon boundary.
TS21: diencephalon Present 6I
Specimen 6O: embryonic day 13.5; Tg(mI56i-cre,EGFP)1Kc/0 (more )
Note: Double labeled: red - Isl1; green - Nkx2-1 (assay MGI:7382853).
Structure Level Pattern Image Note
TS21: diencephalon Present 6O
TS21: telencephalon Present 6O
TS21: forebrain Present Regionally restricted 6O Positive cells extend caudally to where thalamocortical axons (asterisk) cross the diencephalon-telencephalon boundary.
Specimen 6P: embryonic day 13.5; Mapk10tm1Flv/Mapk10tm1Flv, Mapk8tm1Rjd/Mapk8tm1Rjd, Mapk9tm1Flv/Mapk9tm1Flv, Tg(mI56i-cre,EGFP)1Kc/0 (more )
Note: Conditional mutant. Double labeled: red - Isl1; green - Nkx2-1 (assay MGI:7382853).
Structure Level Pattern Image Note
TS21: forebrain Present Regionally restricted 6P Positive cells stopped rostrally and no thalamocortical axons had crossed the diencephalon-telencephalon boundary.
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Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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03/25/2025
MGI 6.24 		The Jackson Laboratory