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Gene Expression Data
Assay Details
Assay
Reference: J:322165 Cunningham JG, et al., Early construction of the thalamocortical axon pathway requires c-Jun N-terminal kinase signaling within the ventral forebrain. Dev Dyn. 2022 Mar;251(3):459-480
Assay type: Immunohistochemistry
MGI Accession ID: MGI:7382853
Gene symbol: Nkx2-1
Gene name: NK2 homeobox 1
Antibody: Anti-TTF-1 (H-190) sc-13040
Detection system: Secondary antibody coupled to Alexa Fluor 488
Results
Specimen 6B: embryonic day 13.5; Tg(mI56i-cre,EGFP)1Kc/0 (more )
Note: Double labeled: green - Nkx2-1; red - Isl1 (assay MGI:7382852).
Structure Level Pattern Image Note
TS21: medial ganglionic eminence Present 6B
TS21: telencephalon Present Regionally restricted 6B Expressed in the globus pallidus.
Specimen 6C: embryonic day 13.5; Tg(mI56i-cre,EGFP)1Kc/0 (more )
Note: Double labeled: green - Nkx2-1; red - Isl1 (assay MGI:7382852).
Structure Level Pattern Image Note
TS21: medial ganglionic eminence Present 6C
TS21: telencephalon Present Regionally restricted 6C Expressed in the globus pallidus.
Specimen 6D: embryonic day 13.5; Tg(mI56i-cre,EGFP)1Kc/0 (more )
Note: Double labeled: green - Nkx2-1; red - Isl1 (assay MGI:7382852).
Structure Level Pattern Image Note
TS21: forebrain Present Regionally restricted 6D Positive globus pallidus cells were present throughout the corridor region and resided directly beneath the thalamocortical axons as they entered the telencephalon.
Specimen 6E: embryonic day 13.5; Tg(mI56i-cre,EGFP)1Kc/0 (more )
Note: Double labeled: green - Nkx2-1; red - Isl1 (assay MGI:7382852).
Structure Level Pattern Image Note
TS21: forebrain Present Regionally restricted 6E Positive globus pallidus cells were present throughout the corridor region and resided directly beneath the thalamocortical axons as they entered the telencephalon. Precisely positioned at the point of axon crossing at the diencephalon-telencephalon boundary.
Specimen 6F: embryonic day 13.5; Mapk10tm1Flv/Mapk10tm1Flv, Mapk8tm1Rjd/Mapk8tm1Rjd, Mapk9tm1Flv/Mapk9tm1Flv, Tg(mI56i-cre,EGFP)1Kc/0 (more )
Note: Conditional mutant. Double labeled: green - Nkx2-1; red - Isl1 (assay MGI:7382852).
Structure Level Pattern Image Note
TS21: forebrain Present Regionally restricted 6F Positive globus pallidus cells occupied a larger territory at rostral levels, extending to the lateral, pial margin of the ventral telencephalon.
Specimen 6G: embryonic day 13.5; Mapk10tm1Flv/Mapk10tm1Flv, Mapk8tm1Rjd/Mapk8tm1Rjd, Mapk9tm1Flv/Mapk9tm1Flv, Tg(mI56i-cre,EGFP)1Kc/0 (more )
Note: Conditional mutant. Double labeled: green - Nkx2-1; red - Isl1 (assay MGI:7382852).
Structure Level Pattern Image Note
TS21: forebrain Present Regionally restricted 6G Slightly more caudal, the positive globus pallidus domain shifted dorsal and lateral, in some cases splitting into multiple clusters with the apparent influx of ectopically positioned Isl1 positive cells.
Specimen 6H: embryonic day 13.5; Mapk10tm1Flv/Mapk10tm1Flv, Mapk8tm1Rjd/Mapk8tm1Rjd, Mapk9tm1Flv/Mapk9tm1Flv, Tg(mI56i-cre,EGFP)1Kc/0 (more )
Note: Conditional mutant. Double labeled: green - Nkx2-1; red - Isl1 (assay MGI:7382852).
Structure Level Pattern Image Note
TS21: forebrain Present Regionally restricted 6H Positive cells were missing from the caudal corridor compared with the control.
Specimen 6I: embryonic day 13.5; Mapk10tm1Flv/Mapk10tm1Flv, Mapk8tm1Rjd/Mapk8tm1Rjd, Mapk9tm1Flv/Mapk9tm1Flv, Tg(mI56i-cre,EGFP)1Kc/0 (more )
Note: Conditional mutant. Double labeled: green - Nkx2-1; red - Isl1 (assay MGI:7382852).
Structure Level Pattern Image Note
TS21: forebrain Present Regionally restricted 6I Positive cells were missing from the caudal corridor compared with the control, ectopically infiltrated by Isl1-positive cells atmid-levels. Positive cells failed to extend caudally to the diencephalon-telencephalon boundary.
Specimen 6O: embryonic day 13.5; Tg(mI56i-cre,EGFP)1Kc/0 (more )
Note: Double labeled: green - Nkx2-1; red - Isl1 (assay MGI:7382852).
Structure Level Pattern Image Note
TS21: telencephalon Present Regionally restricted 6O Precisely positioned at the point of axon crossing at the diencephalon-telencephalon boundary.
Specimen 6P: embryonic day 13.5; Mapk10tm1Flv/Mapk10tm1Flv, Mapk8tm1Rjd/Mapk8tm1Rjd, Mapk9tm1Flv/Mapk9tm1Flv, Tg(mI56i-cre,EGFP)1Kc/0 (more )
Note: Conditional mutant. Double labeled: green - Nkx2-1; red - Isl1 (assay MGI:7382852).
Structure Level Pattern Image Note
TS21: telencephalon Present Regionally restricted 6P Positive cells failed to extend caudally to the diencephalon-telencephalon boundary.
Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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04/30/2024
MGI 6.23 		The Jackson Laboratory