Cdh1 Immunohistochemistry Gene Expression Assay

Gene Expression Data
Assay Details

Assay

Reference:	J:327630 Li R, et al., Generation of an Ihh-mKate2-Dre knock-in mouse line. Genesis. 2022 Jul;60(6-7):e23488
Assay type:	Immunohistochemistry
MGI Accession ID:	MGI:7343689
Gene symbol:	Cdh1
Gene name:	cadherin 1

Antibody:	anti-E-Cadherin (24E10; #3195)
Detection system:	Secondary antibody coupled to horseradish peroxidase
Assay notes:	Staining was visualized using tyramide-conjugated fluorophores (NEON 4-color Allround Discovery Kit for FFPE, Histova Biotechnology, NEFP450).

Results

S3c E-cadherin ©

S3c Merge ©

Specimen S3c: postnatal day 90; Gt(ROSA)26Sor^{tm10(CAG-Confetti2)Bzsh}/Gt(ROSA)26Sor⁺, Ihh^{em1(Tomm20/mKate2,dre)Xya}/Ihh⁺ (more

)
Note: Conditional mutant. Multi-labeled: yellow - Cdh1; red - Ihh reporter (assay MGI:7341718); magenta - Sycp3 (assay MGI:7343686); green - Kit (assay MGI:7343687); cyan - Dre reporter.

Structure	Level	Pattern	Image	Note
TS28: spermatogonium	Present	Regionally restricted	S3c E-cadherin , S3c Merge , S3c Merge inset	Expression is seen in undifferentiated spermatogonia.

Contributing Projects: Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
Citing These Resources Funding Information Warranty Disclaimer, Privacy Notice, Licensing, & Copyright Send questions and comments to User Support.	last database update 05/14/2024 MGI 6.23