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Gene Expression Data
Assay Details
Assay
Reference: J:327630 Li R, et al., Generation of an Ihh-mKate2-Dre knock-in mouse line. Genesis. 2022 Jul;60(6-7):e23488
Assay type: In situ reporter (knock in)
MGI Accession ID: MGI:7341718
Gene symbol: Ihh
Gene name: Indian hedgehog
Reporter: RFP
Antibody: anti-RFP (R10367)
Detection system: Secondary antibody coupled to horseradish peroxidase
Assay notes: Staining was visualized using tyramide-conjugated fluorophores (NEON 4-color Allround Discovery Kit for FFPE, Histova Biotechnology, NEFP450).
Results
Specimen 2a - P1: postnatal day 1; Ihhem1(Tomm20/mKate2,dre)Xya/Ihh+ (more )
Structure Cell Type Level Pattern Image Note
TS27: femur growth plate cartilage chondrocyte Present 2a left Expression is seen in pre-hypertrophic and hypertrophic chondrocytes.
TS27: femur hypertrophic chondrocyte Present 2a left Expression is seen in pre-hypertrophic and hypertrophic chondrocytes.
TS27: hypertrophic cartilage zone growth plate cartilage chondrocyte Present 2a left Expression is seen in pre-hypertrophic and hypertrophic chondrocytes.
TS27: hypertrophic cartilage zone hypertrophic chondrocyte Present 2a left Expression is seen in pre-hypertrophic and hypertrophic chondrocytes.
Specimen 2a -P7: postnatal day 7; Ihhem1(Tomm20/mKate2,dre)Xya/Ihh+ (more )
Structure Cell Type Level Pattern Image Note
TS28: femur growth plate cartilage chondrocyte Present 2a right Expression is seen in pre-hypertrophic and hypertrophic chondrocytes.
TS28: femur hypertrophic chondrocyte Present 2a right Expression is seen in pre-hypertrophic and hypertrophic chondrocytes.
TS28: hypertrophic cartilage zone growth plate cartilage chondrocyte Present 2a right Expression is seen in pre-hypertrophic and hypertrophic chondrocytes.
TS28: hypertrophic cartilage zone hypertrophic chondrocyte Present 2a right Expression is seen in pre-hypertrophic and hypertrophic chondrocytes.
Specimen 3a/c: postnatal day 120; Gt(ROSA)26Sortm10(CAG-Confetti2)Bzsh/Gt(ROSA)26Sor+, Ihhem1(Tomm20/mKate2,dre)Xya/Ihh+ (more )
Note: Conditional mutant. Double labeled: red - Ihh reporter; cyan - Dre reporter.
Structure Cell Type Level Pattern Image Note
TS28: follicle cell of preantral follicle granulosa cell Present 3a , 3c
TS28: oocyte of preantral follicle Absent 3c , 3a The apparent staining in the oocyte is non-specific.
Specimen 3d/f: postnatal day 120; Gt(ROSA)26Sortm10(CAG-Confetti2)Bzsh/Gt(ROSA)26Sor+, Ihhem1(Tomm20/mKate2,dre)Xya/Ihh+ (more )
Note: Conditional mutant. Double labeled: red - Ihh reporter; cyan - Dre reporter.
Structure Cell Type Level Pattern Image Note
TS28: follicle cell of antral follicle granulosa cell Present 3f , 3d
TS28: oocyte of antral follicle Absent 3d , 3f The apparent staining in the oocyte is non-specific.
Specimen 3g/i: postnatal day 120; Gt(ROSA)26Sortm10(CAG-Confetti2)Bzsh/Gt(ROSA)26Sor+, Ihhem1(Tomm20/mKate2,dre)Xya/Ihh+ (more )
Note: Conditional mutant. Double labeled: red - Ihh reporter; cyan - Dre reporter.
Structure Level Pattern Image Note
TS28: corpus luteum Absent 3i , 3g
Specimen 3j/l: postnatal day 120; Gt(ROSA)26Sortm10(CAG-Confetti2)Bzsh/Gt(ROSA)26Sor+, Ihhem1(Tomm20/mKate2,dre)Xya/Ihh+ (more )
Note: Conditional mutant. Double labeled: red - Ihh reporter; cyan - Dre reporter.
Structure Level Pattern Image Note
TS28: corpus luteum Absent 3l , 3j
Specimen 4a/c: postnatal day 21; Gt(ROSA)26Sortm10(CAG-Confetti2)Bzsh/Gt(ROSA)26Sor+, Ihhem1(Tomm20/mKate2,dre)Xya/Ihh+ (more )
Note: Conditional mutant. Double labeled: red - Ihh reporter; cyan - Dre reporter.
Structure Level Pattern Image Note
TS28: choroidal vasculature Absent 4c , 4a There is no expression in the choroidal endothelium.
Specimen 4d/f: postnatal day 120; Gt(ROSA)26Sortm10(CAG-Confetti2)Bzsh/Gt(ROSA)26Sor+, Ihhem1(Tomm20/mKate2,dre)Xya/Ihh+ (more )
Note: Conditional mutant. Double labeled: red - Ihh reporter; cyan - Dre reporter.
Structure Level Pattern Image Note
TS28: choroidal vasculature Absent 4f , 4d There is no expression in the choroidal endothelium.
Specimen 4g/i: postnatal day 21; Gt(ROSA)26Sortm10(CAG-Confetti2)Bzsh/Gt(ROSA)26Sor+, Ihhem1(Tomm20/mKate2,dre)Xya/Ihh+ (more )
Note: Conditional mutant. Double labeled: red - Ihh reporter; cyan - Dre reporter.
Structure Level Pattern Image Note
TS28: retina ganglion cell layer Present 4g , 4i
TS28: retina inner nuclear layer Present Regionally restricted 4i , 4g Expression is seen in some cells.
Specimen 4j/l: postnatal day 120; Gt(ROSA)26Sortm10(CAG-Confetti2)Bzsh/Gt(ROSA)26Sor+, Ihhem1(Tomm20/mKate2,dre)Xya/Ihh+ (more )
Note: Conditional mutant. Double labeled: red - Ihh reporter; cyan - Dre reporter.
Structure Level Pattern Image Note
TS28: retina ganglion cell layer Present 4l , 4j
TS28: retina inner nuclear layer Present Regionally restricted 4j , 4l Expression is seen in some cells.
Specimen 5a/c: postnatal day 21; Gt(ROSA)26Sortm10(CAG-Confetti2)Bzsh/Gt(ROSA)26Sor+, Ihhem1(Tomm20/mKate2,dre)Xya/Ihh+ (more )
Note: Conditional mutant. Double labeled: red - Ihh reporter; cyan - Dre reporter.
Structure Cell Type Level Pattern Image Note
TS28: cerebellum Purkinje cell layer Purkinje cell Present 5a , 5c
Specimen 5d/f: postnatal day 120; Gt(ROSA)26Sortm10(CAG-Confetti2)Bzsh/Gt(ROSA)26Sor+, Ihhem1(Tomm20/mKate2,dre)Xya/Ihh+ (more )
Note: Conditional mutant. Double labeled: red - Ihh reporter; cyan - Dre reporter.
Structure Cell Type Level Pattern Image Note
TS28: cerebellum Purkinje cell layer Purkinje cell Present 5f , 5d
Specimen 5g/i: postnatal day 120; Gt(ROSA)26Sortm10(CAG-Confetti2)Bzsh/Gt(ROSA)26Sor+, Ihhem1(Tomm20/mKate2,dre)Xya/Ihh+ (more )
Note: Conditional mutant. Double labeled: red - Ihh reporter; cyan - Dre reporter.
Structure Level Pattern Image Note
TS28: gallbladder epithelium Present 5g , 5i Expression is seen in the columnar epithelium.
Specimen 6a: postnatal day 1; Gt(ROSA)26Sortm10(CAG-Confetti2)Bzsh/Gt(ROSA)26Sor+, Ihhem1(Tomm20/mKate2,dre)Xya/Ihh+ (more )
Note: Conditional mutant.
Structure Level Pattern Image Note
TS27: bladder Present Regionally restricted 6a Expression is seen in a few cells.
Specimen 6b: postnatal day 1; Gt(ROSA)26Sortm10(CAG-Confetti2)Bzsh/Gt(ROSA)26Sor+, Ihhem1(Tomm20/mKate2,dre)Xya/Ihh+ (more )
Note: Conditional mutant.
Structure Level Pattern Image Note
TS27: metanephros Present Regionally restricted 6b Expression is seen in a few cells.
Specimen 6c: postnatal day 1; Gt(ROSA)26Sortm10(CAG-Confetti2)Bzsh/Gt(ROSA)26Sor+, Ihhem1(Tomm20/mKate2,dre)Xya/Ihh+ (more )
Note: Conditional mutant.
Structure Level Pattern Image Note
TS27: liver Present Regionally restricted 6c Expression is seen in a few cells.
Specimen 6d: postnatal day 120; Gt(ROSA)26Sortm10(CAG-Confetti2)Bzsh/Gt(ROSA)26Sor+, Ihhem1(Tomm20/mKate2,dre)Xya/Ihh+ (more )
Note: Conditional mutant.
Structure Level Pattern Image Note
TS28: skin Present Single cells 6d
Specimen 6e: postnatal day 120; Gt(ROSA)26Sortm10(CAG-Confetti2)Bzsh/Gt(ROSA)26Sor+, Ihhem1(Tomm20/mKate2,dre)Xya/Ihh+ (more )
Note: Conditional mutant.
Structure Level Pattern Image Note
TS28: islets of Langerhans Present 6e
Specimen 6f: postnatal day 120; Gt(ROSA)26Sortm10(CAG-Confetti2)Bzsh/Gt(ROSA)26Sor+, Ihhem1(Tomm20/mKate2,dre)Xya/Ihh+ (more )
Note: Conditional mutant.
Structure Level Pattern Image Note
TS28: thymus Present Regionally restricted 6f Expression is seen in a few cells.
Specimen S3b: postnatal day 120; Gt(ROSA)26Sortm10(CAG-Confetti2)Bzsh/Gt(ROSA)26Sor+, Ihhem1(Tomm20/mKate2,dre)Xya/Ihh+ (more )
Note: Conditional mutant. Multi-labeled: red - Ihh reporter; magenta - Cd207 (assay MGI:7343677); green - Cd3e (assay MGI:7343679); yellow - S100a1 (assay MGI:7343682); cyan - Dre reporter.
Structure Cell Type Level Pattern Image Note
TS28: skin Langerhans cell Present Single cells S3b mKate2 , S3b Merge inset , S3b Merge
Specimen S3c: postnatal day 90; Gt(ROSA)26Sortm10(CAG-Confetti2)Bzsh/Gt(ROSA)26Sor+, Ihhem1(Tomm20/mKate2,dre)Xya/Ihh+ (more )
Note: Conditional mutant. Multi-labeled: red - Ihh reporter;magenta - Sycp3 (assay MGI:7343686); green - Kit (assay MGI:7343687); yellow - Cdh1 (assay MGI:7343689); cyan - Dre reporter.
Structure Level Pattern Image Note
TS28: seminiferous tubule Absent S3c mKate2 , S3c Merge inset , S3c Merge
Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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04/30/2024
MGI 6.23 		The Jackson Laboratory