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Gene Expression Data
Assay Details
Assay
Reference: J:327630 Li R, et al., Generation of an Ihh-mKate2-Dre knock-in mouse line. Genesis. 2022 Jul;60(6-7):e23488
Assay type: Immunohistochemistry
MGI Accession ID: MGI:7343687
Gene symbol: Kit
Gene name: KIT proto-oncogene receptor tyrosine kinase
Antibody: anti-c-Kit [EPR22566-205] (ab231780)
Detection system: Secondary antibody coupled to horseradish peroxidase
Assay notes: Staining was visualized using tyramide-conjugated fluorophores (NEON 4-color Allround Discovery Kit for FFPE, Histova Biotechnology, NEFP450).
Results
Specimen S3c: postnatal day 90; Gt(ROSA)26Sortm10(CAG-Confetti2)Bzsh/Gt(ROSA)26Sor+, Ihhem1(Tomm20/mKate2,dre)Xya/Ihh+ (more )
Note: Conditional mutant. Multi-labeled: green - Kit; red - Ihh reporter (assay MGI:7341718); magenta - Sycp3 (assay MGI:7343686); yellow - Cdh1 (assay MGI:7343687); cyan - Dre reporter.
Structure Level Pattern Image Note
TS28: spermatogonium Present Regionally restricted S3c c-Kit , S3c Merge inset , S3c Merge Expression is seen in differentiated spermatogonia.
Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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05/14/2024
MGI 6.23 		The Jackson Laboratory