Sycp3 Immunohistochemistry Gene Expression Assay

Gene Expression Data
Assay Details

Assay

Reference:	J:327630 Li R, et al., Generation of an Ihh-mKate2-Dre knock-in mouse line. Genesis. 2022 Jul;60(6-7):e23488
Assay type:	Immunohistochemistry
MGI Accession ID:	MGI:7343686
Gene symbol:	Sycp3
Gene name:	synaptonemal complex protein 3

Antibody:	anti-SCP3 [Cor 10G11/7] (ab97672)
Detection system:	Secondary antibody coupled to horseradish peroxidase
Assay notes:	Staining was visualized using tyramide-conjugated fluorophores (NEON 4-color Allround Discovery Kit for FFPE, Histova Biotechnology, NEFP450).

Results

S3c Merge ©

S3c Merge inset ©

S3c SPC3 ©

Specimen S3c: postnatal day 90; Gt(ROSA)26Sor^{tm10(CAG-Confetti2)Bzsh}/Gt(ROSA)26Sor⁺, Ihh^{em1(Tomm20/mKate2,dre)Xya}/Ihh⁺ (more

)
Note: Conditional mutant. Multi-labeled: magenta - Sycp3; red - Ihh reporter (assay MGI:7341718); green - Kit (assay MGI:7343687); yellow - Cdh1 (assay MGI:7343697); cyan - Dre reporter.

Structure	Level	Pattern	Image	Note
TS28: spermatocyte	Present		S3c Merge inset , S3c Merge , S3c SPC3

Contributing Projects: Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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