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Gene Expression Data
Assay Details
Assay
Reference: J:264603 Da Silva F, et al., Myocardial-specific R-spondin3 drives proliferation of the coronary stems primarily through the Leucine Rich Repeat G Protein coupled receptor LGR4. Dev Biol. 2018 Sep 1;441(1):42-51
Assay type: Immunohistochemistry
MGI Accession ID: MGI:7336837
Gene symbol: Tagln
Gene name: transgelin
Antibody: SM22alpha antibody
Detection system: Secondary antibody coupled to Alexa Fluor 555
Results
Specimen 5C WT: embryonic day 16.5 (more )
Note: Double labeled: red - Tagln; green - Pecam1 (assay MGI:7336835).
Structure Cell Type Level Pattern Image Note
TS24: coronary artery smooth muscle cell of the coronary artery Present 5C upper left , 5C upper right
Specimen 5C KO: embryonic day 16.5; Lgr4em1Asc/Lgr4em1Asc (more )
Note: Double labeled: red - Tagln; green - Pecam1 (assay MGI:7336835).
Structure Cell Type Level Pattern Image Note
TS24: coronary artery smooth muscle cell of the coronary artery Present 5C lower left , 5C lower right Reduction in coronary arteries was seen (quantified in 5D).
Specimen S1B Pax3-cKO: embryonic day 16.5; Gt(ROSA)26Sortm4(ACTB-tdTomato,-EGFP)Luo/Gt(ROSA)26Sor+, Pax3tm1(cre)Joe/Pax3+, Rspo3tm2Okam/Rspo3tm2Okam (more )
Note: Conditional mutant. Double labeled: red - Tagln; green - Pecam1 (assay MGI:7336835).
Structure Cell Type Level Pattern Image Note
TS24: coronary artery smooth muscle cell of the coronary artery Present S1B left Coronary artery formation was not affected.
Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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03/18/2025
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