About   Help   FAQ
Gene Expression Data
Assay Details
Assay
Reference: J:264603 Da Silva F, et al., Myocardial-specific R-spondin3 drives proliferation of the coronary stems primarily through the Leucine Rich Repeat G Protein coupled receptor LGR4. Dev Biol. 2018 Sep 1;441(1):42-51
Assay type: Immunohistochemistry
MGI Accession ID: MGI:7336835
Gene symbol: Pecam1
Gene name: platelet/endothelial cell adhesion molecule 1
Antibody: anti-PECAM
Detection system: Secondary antibody coupled to Alexa Fluor 488
Results
Specimen 1Aiv: embryonic day 14.5 (more )
Note: Double labeled: green - Pecam1; red - Rspo3 (assay MGI:7336784).
Structure Cell Type Level Pattern Image Note
TS22: coronary artery endothelial cell of coronary artery Present 1Aiv
Specimen 5C WT: embryonic day 16.5 (more )
Note: Double labeled: green - Pecam1; red - Tagln (assay MGI:7336837).
Structure Cell Type Level Pattern Image Note
TS24: coronary artery endothelial cell of coronary artery Present 5C upper left , 5C upper right
Specimen 5C KO: embryonic day 16.5; Lgr4em1Asc/Lgr4em1Asc (more )
Note: Double labeled: green - Pecam1; red - Tagln (assay MGI:7336837).
Structure Cell Type Level Pattern Image Note
TS24: coronary artery endothelial cell of coronary artery Present 5C lower left , 5C lower right Reduction in coronary arteries was seen (quantified in 5D).
Specimen 5F WT: embryonic day 13.5 (more )
Note: Double labeled: green - Pecam1; red - anti-BrdU antibody.
Structure Cell Type Level Pattern Image Note
TS21: coronary artery endothelial cell of coronary artery Present 5F upper right , 5F upper left
Specimen 5F KO: embryonic day 13.5; Lgr4em1Asc/Lgr4em1Asc (more )
Note: Double labeled: green - Pecam1; red - anti-BrdU antibody.
Structure Cell Type Level Pattern Image Note
TS21: coronary artery endothelial cell of coronary artery Present 5F lower right , 5F lower left Decreased rates of endothelial proliferation (BrdU+) in the mutant.
Specimen S1B Pax3-cKO: embryonic day 16.5; Gt(ROSA)26Sortm4(ACTB-tdTomato,-EGFP)Luo/Gt(ROSA)26Sor+, Pax3tm1(cre)Joe/Pax3+, Rspo3tm2Okam/Rspo3tm2Okam (more )
Note: Conditional mutant. Double labeled: green - Pecam1; red - Tagln (assay MGI:7336837).
Structure Cell Type Level Pattern Image Note
TS24: coronary artery endothelial cell of coronary artery Present S1B left Coronary artery formation was not affected.
Specimen S3B right: postnatal adult (more )
Note: Double labeled: green - Pecam1; red - Lgr4 (assay MGI:7336791).
Structure Cell Type Level Pattern Image Note
TS28: heart endothelium endothelial cell Present S3B right
Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
Citing These Resources
Funding Information
Warranty Disclaimer, Privacy Notice, Licensing, & Copyright
Send questions and comments to User Support. 		last database update
03/18/2025
MGI 6.24 		The Jackson Laboratory