Fgf8 RNA in situ Gene Expression Assay

Gene Expression Data
Assay Details

Assay

Reference:	J:219823 Rosin JM, et al., Mice lacking the transcription factor SHOX2 display impaired cerebellar development and deficits in motor coordination. Dev Biol. 2015 Mar 1;399(1):54-67
Assay type:	RNA in situ
MGI Accession ID:	MGI:7336386
Gene symbol:	Fgf8
Gene name:	fibroblast growth factor 8

Probe:	Fgf8 cDNA13
Probe preparation:	Antisense labelled with digoxigenin RNA
Visualized with:	Alkaline phosphatase

Results

3A ©

Specimen 3A: embryonic day 12.5 (more

)

Structure	Level	Pattern	Image	Note
TS20: midbrain-hindbrain junction	Present		3A	isthmus

3B ©

Specimen 3B: embryonic day 12.5; Shox2^tm1Ddu/Shox2^tm1.1Ddu, Tg(Nes-cre)1Kln/0 (more

)
Note: Conditional mutant.

Structure	Level	Pattern	Image	Note
TS20: midbrain-hindbrain junction	Present		3B	Only subtle changes were seen in the isthmus.

S3G ©

Specimen S3G: embryonic day 10.5 (more

)

Structure	Level	Pattern	Image	Note
TS17: midbrain-hindbrain junction	Present		S3G	isthmus

S3H ©

Specimen S3H: embryonic day 10.5; Shox2^tm1Ddu/Shox2^tm1.1Ddu, Tg(Nes-cre)1Kln/0 (more

)
Note: Conditional mutant.

Structure	Level	Pattern	Image	Note
TS17: midbrain-hindbrain junction	Present		S3H	Only subtle changes were seen in the isthmus.

S3I ©

Specimen S3I: embryonic day 10.5 (more

)

Structure	Level	Pattern	Image	Note
TS17: midbrain-hindbrain junction	Present		S3I	isthmus

S3J ©

Specimen S3J: embryonic day 10.5; Shox2^tm1Ddu/Shox2^tm1.1Ddu, Tg(Nes-cre)1Kln/0 (more

)
Note: Conditional mutant.

Structure	Level	Pattern	Image	Note
TS17: midbrain-hindbrain junction	Present		S3J	Only subtle changes were seen in the isthmus.

S3K ©

Specimen S3K: embryonic day 10.5 (more

)

Structure	Level	Pattern	Image	Note
TS17: midbrain-hindbrain junction	Present		S3K	isthmus

S3L ©

Specimen S3L: embryonic day 10.5; Shox2^tm1Ddu/Shox2^tm1.1Ddu, Tg(Nes-cre)1Kln/0 (more

)
Note: Conditional mutant.

Structure	Level	Pattern	Image	Note
TS17: midbrain-hindbrain junction	Present		S3L	Only subtle changes were seen in the isthmus.

S3M ©

Specimen S3M: embryonic day 11.5 (more

)

Structure	Level	Pattern	Image	Note
TS19: midbrain-hindbrain junction	Present		S3M	isthmus

S3N ©

Specimen S3N: embryonic day 11.5; Shox2^tm1Ddu/Shox2^tm1.1Ddu, Tg(Nes-cre)1Kln/0 (more

)
Note: Conditional mutant.

Structure	Level	Pattern	Image	Note
TS19: midbrain-hindbrain junction	Present		S3N	Only subtle changes were seen in the isthmus.

S3O ©

Specimen S3O: embryonic day 11.5 (more

)

Structure	Level	Pattern	Image	Note
TS19: midbrain-hindbrain junction	Present		S3O	isthmus

S3P ©

Specimen S3P: embryonic day 11.5; Shox2^tm1Ddu/Shox2^tm1.1Ddu, Tg(Nes-cre)1Kln/0 (more

)
Note: Conditional mutant.

Structure	Level	Pattern	Image	Note
TS19: midbrain-hindbrain junction	Present		S3P	Only subtle changes were seen in the isthmus.

S3Q ©

Specimen S3Q: embryonic day 11.5 (more

)

Structure	Level	Pattern	Image	Note
TS19: midbrain-hindbrain junction	Present		S3Q	isthmus

S3R ©

Specimen S3R: embryonic day 11.5; Shox2^tm1Ddu/Shox2^tm1.1Ddu, Tg(Nes-cre)1Kln/0 (more

)
Note: Conditional mutant.

Structure	Level	Pattern	Image	Note
TS19: midbrain-hindbrain junction	Present		S3R	Only subtle changes were seen in the isthmus.

Contributing Projects: Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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