About   Help   FAQ
Gene Expression Data
Assay Details
Assay
Reference: J:203995 Lee S, et al., Forward genetics identifies Kdf1/1810019J16Rik as an essential regulator of the proliferation-differentiation decision in epidermal progenitor cells. Dev Biol. 2013 Nov 15;383(2):201-13
Assay type: Immunohistochemistry
MGI Accession ID: MGI:7333008
Gene symbol: Flg
Gene name: filaggrin
Results
Specimen 2C control: embryonic day 18.5 (more )
Note: Double labeled: red - Flg; green - Itgb4 (assay MGI:7333006).
Structure Cell Type Level Pattern Image Note
TS26: epidermis cornified layer keratinocyte Present 2C left
TS26: epidermis basal layer Absent 2C left
Specimen 2C -/-: embryonic day 18.5; Kdf1shd/Kdf1shd (more )
Note: Double labeled: red - Flg; green - Itgb4 (assay MGI:7333006).
Structure Cell Type Level Pattern Image Note
TS26: epidermis cornified layer keratinocyte Ambiguous 2C right Expression was strongly reduced or absent.
TS26: epidermis basal layer Absent 2C right
Specimen 3I control: embryonic day 18.5 (more )
Note: Double labeled: red - Flg; green - Itgb4 (assay MGI:7333006).
Structure Cell Type Level Pattern Image Note
TS26: epidermis cornified layer keratinocyte Present 3I left
TS26: epidermis basal layer Absent 3I left
Specimen 3I shd+/-; Er+/-: embryonic day 18.5; Kdf1shd/Kdf1+, SfnEr/Sfn+ (more )
Note: Double labeled: red - Flg; green - Itgb4 (assay MGI:7333006).
Structure Cell Type Level Pattern Image Note
TS26: epidermis keratinocyte Present Regionally restricted 3I right The epidermis was greatly thickened and the cornified layer displayed gaps, with positive keratinocytes spilling out onto the surface.
TS26: epidermis basal layer Absent 3I right
Specimen 4K control: embryonic day 18.5 (more )
Note: Lateral back skin. Double labeled: red - Flg; green - Itgb4 (assay MGI:7333006).
Structure Cell Type Level Pattern Image Note
TS26: epidermis cornified layer keratinocyte Present 4K left
Specimen 4K shd-/-; p63+/-: embryonic day 18.5; Kdf1shd/Kdf1shd, Trp63tm2Brd/Trp63+ (more )
Note: Lateral back skin. Double labeled: red - Flg; green - Itgb4 (assay MGI:7333006).
Structure Cell Type Level Pattern Image Note
TS26: epidermis cornified layer keratinocyte Present 4K right Normal thickness and expression was restored in lateral (barrier-restored) regions.
Specimen 6E control: embryonic day 18.5 (more )
Note: Double labeled: red - Flg; green - Itgb4 (assay MGI:7333006).
Structure Cell Type Level Pattern Image Note
TS26: epidermis cornified layer keratinocyte Present 6E left
Specimen 6E GT/shd: embryonic day 18.5; Kdf1shd/Kdf1tm1a(EUCOMM)Wtsi (more )
Note: Double labeled: red - Flg; green - Itgb4 (assay MGI:7333006).
Structure Cell Type Level Pattern Image Note
TS26: epidermis keratinocyte Absent 6E middle Expression was not induced.
Specimen 6E GT/GT: embryonic day 18.5; Kdf1tm1a(EUCOMM)Wtsi/Kdf1tm1a(EUCOMM)Wtsi (more )
Note: Double labeled: red - Flg; green - Itgb4 (assay MGI:7333006).
Structure Cell Type Level Pattern Image Note
TS26: epidermis keratinocyte Absent 6E right Expression was not induced.
Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
Citing These Resources
Funding Information
Warranty Disclaimer, Privacy Notice, Licensing, & Copyright
Send questions and comments to User Support. 		last database update
03/25/2025
MGI 6.24 		The Jackson Laboratory