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Gene Expression Data
Assay Details
Assay
Reference: J:203995 Lee S, et al., Forward genetics identifies Kdf1/1810019J16Rik as an essential regulator of the proliferation-differentiation decision in epidermal progenitor cells. Dev Biol. 2013 Nov 15;383(2):201-13
Assay type: Immunohistochemistry
MGI Accession ID: MGI:7333008
Gene symbol: Flg
Gene name: filaggrin
Results
Specimen 2C control: embryonic day 18.5 (more )
Note: Double labeled: red - Flg; green - Itgb4 (assay MGI:7333006).
Structure Cell Type Level Pattern Image Note
TS26: epidermis cornified layer keratinocyte Present 2C left
TS26: epidermis basal layer Absent 2C left
Specimen 2C -/-: embryonic day 18.5; Kdf1shd/Kdf1shd (more )
Note: Double labeled: red - Flg; green - Itgb4 (assay MGI:7333006).
Structure Cell Type Level Pattern Image Note
TS26: epidermis cornified layer keratinocyte Ambiguous 2C right Expression was strongly reduced or absent.
TS26: epidermis basal layer Absent 2C right
Specimen 3I control: embryonic day 18.5 (more )
Note: Double labeled: red - Flg; green - Itgb4 (assay MGI:7333006).
Structure Cell Type Level Pattern Image Note
TS26: epidermis cornified layer keratinocyte Present 3I left
TS26: epidermis basal layer Absent 3I left
Specimen 3I shd+/-; Er+/-: embryonic day 18.5; Kdf1shd/Kdf1+, SfnEr/Sfn+ (more )
Note: Double labeled: red - Flg; green - Itgb4 (assay MGI:7333006).
Structure Cell Type Level Pattern Image Note
TS26: epidermis keratinocyte Present Regionally restricted 3I right The epidermis was greatly thickened and the cornified layer displayed gaps, with positive keratinocytes spilling out onto the surface.
TS26: epidermis basal layer Absent 3I right
Specimen 4K control: embryonic day 18.5 (more )
Note: Lateral back skin. Double labeled: red - Flg; green - Itgb4 (assay MGI:7333006).
Structure Cell Type Level Pattern Image Note
TS26: epidermis cornified layer keratinocyte Present 4K left
Specimen 4K shd-/-; p63+/-: embryonic day 18.5; Kdf1shd/Kdf1shd, Trp63tm2Brd/Trp63+ (more )
Note: Lateral back skin. Double labeled: red - Flg; green - Itgb4 (assay MGI:7333006).
Structure Cell Type Level Pattern Image Note
TS26: epidermis cornified layer keratinocyte Present 4K right Normal thickness and expression was restored in lateral (barrier-restored) regions.
Specimen 6E control: embryonic day 18.5 (more )
Note: Double labeled: red - Flg; green - Itgb4 (assay MGI:7333006).
Structure Cell Type Level Pattern Image Note
TS26: epidermis cornified layer keratinocyte Present 6E left
Specimen 6E GT/shd: embryonic day 18.5; Kdf1shd/Kdf1tm1a(EUCOMM)Wtsi (more )
Note: Double labeled: red - Flg; green - Itgb4 (assay MGI:7333006).
Structure Cell Type Level Pattern Image Note
TS26: epidermis keratinocyte Absent 6E middle Expression was not induced.
Specimen 6E GT/GT: embryonic day 18.5; Kdf1tm1a(EUCOMM)Wtsi/Kdf1tm1a(EUCOMM)Wtsi (more )
Note: Double labeled: red - Flg; green - Itgb4 (assay MGI:7333006).
Structure Cell Type Level Pattern Image Note
TS26: epidermis keratinocyte Absent 6E right Expression was not induced.
Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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05/21/2024
MGI 6.23 		The Jackson Laboratory