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Gene Expression Data
Assay Details
Assay
Reference: J:173795 Hilliard S, et al., Tight regulation of p53 activity by Mdm2 is required for ureteric bud growth and branching. Dev Biol. 2011 May 15;353(2):354-66
Assay type: Immunohistochemistry
MGI Accession ID: MGI:7330268
Gene symbol: Wt1
Gene name: WT1 transcription factor
Antibody: anti-WT1 (C-19; sc-192)
Detection system: Secondary antibody coupled to Alexa Fluor 555
Results
Specimen 5G/H/I: embryonic day 14.5; Mdm2tm1.2Mep/Mdm2tm1.2Mep (more )
Note: Double labeled: red - Wt1; green - cytokeratin.
Structure Cell Type Level Pattern Image Note
TS22: maturing glomerular tuft podocyte Present 5H , 5G , 5I
Specimen 5J/K/L: embryonic day 14.5; Mdm2tm1.2Mep/Mdm2tm1.2Mep, Tg(Hoxb7-cre)5526Cmb/0 (more )
Note: Conditional mutant. Double labeled: red - Wt1; green - cytokeratin.
Structure Cell Type Level Pattern Image Note
TS22: maturing glomerular tuft podocyte Present 5J , 5K , 5L The paucity of positive glomeruli in mutant was seen as compared to the control.
Specimen 9E: embryonic day 13.5; Mdm2tm1.2Mep/Mdm2tm1.2Mep, Trp53tm1Tyj/Trp53+ (more )
Structure Level Pattern Image Note
TS22: maturing glomerular tuft Present 9E
TS22: mesenchyme of metanephros Present 9E
Specimen 9G: embryonic day 13.5; Mdm2tm1.2Mep/Mdm2tm1.2Mep, Tg(Hoxb7-cre)5526Cmb/0, Trp53tm1Tyj/Trp53+ (more )
Note: Conditional mutant.
Structure Level Pattern Image Note
TS22: maturing glomerular tuft Present 9G The paucity of positive glomeruli in mutant was seen as compared to the control.
TS22: mesenchyme of metanephros Present 9G The paucity of positive glomeruli in mutant was seen as compared to the control.
Specimen 9I: embryonic day 13.5; Mdm2tm1.2Mep/Mdm2tm1.2Mep, Tg(Hoxb7-cre)5526Cmb/0, Trp53tm1Tyj/Trp53tm1Tyj (more )
Note: Conditional mutant.
Structure Level Pattern Image Note
TS22: maturing glomerular tuft Present 9I Similar as the wild-type.
TS22: mesenchyme of metanephros Present 9I Similar as the wild-type.
Specimen S3G/H/I: embryonic day 15.5; Mdm2tm1.2Mep/Mdm2tm1.2Mep (more )
Note: Double labeled: red - Wt1; green - cytokeratin.
Structure Cell Type Level Pattern Image Note
TS23: maturing glomerular tuft podocyte Present S3H , S3G , S3I
Specimen S3J/K/L: embryonic day 15.5; Mdm2tm1.2Mep/Mdm2tm1.2Mep, Tg(Hoxb7-cre)5526Cmb/0 (more )
Note: Conditional mutant. Double labeled: red - Wt1; green - cytokeratin.
Structure Cell Type Level Pattern Image Note
TS23: maturing glomerular tuft podocyte Present S3L , S3K , S3J Fewer positive podocyte progenitors and capillary loop glomeruli.
Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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12/30/2025
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