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Gene Expression Data
Assay Details
Assay
Reference: J:171508 Ikegami D, et al., Sox9 sustains chondrocyte survival and hypertrophy in part through Pik3ca-Akt pathways. Development. 2011 Apr;138(8):1507-19
Assay type: RNA in situ
MGI Accession ID: MGI:7286331
Gene symbol: Col2a1
Gene name: collagen, type II, alpha 1
Probe: Col2a1 probe28
Probe preparation: Antisense labelled with digoxigenin RNA
Results
Specimen 1C Sox9flox/+: embryonic day 13.5; Sox9tm2Crm/Sox9+ (more )
Structure Cell Type Level Pattern Image Note
TS21: humerus cartilage condensation chondrocyte Present 1C Col2a1 Sox9flox/+
Specimen 1C 11Enh-Cre; Sox9flox/flox: embryonic day 13.5; Sox9tm2Crm/Sox9tm2Crm, Tg(Col11a2-cre)1Ntsu/0 (more )
Note: Conditional mutant.
Structure Cell Type Level Pattern Image Note
TS21: humerus cartilage condensation chondrocyte Present Regionally restricted 1C Col2a1 11Enh-Cre; Sox9flox/flox Expression was lost in the central region of the cartilage in the conditional mutant.
Specimen 3A Sox9flox/+: embryonic day 13.5; Sox9tm2Crm/Sox9+ (more )
Note: Proximal humerus.
Structure Level Pattern Image Note
TS21: humerus cartilage condensation Present 3A Col2a1 Sox9flox/+
Specimen 3A 11Prom-Cre; Sox9flox/flox: embryonic day 13.5; Sox9tm2Crm/Sox9tm2Crm, Tg(Col11a2-cre)2Ntsu/0 (more )
Note: Conditional mutant. Proximal humerus.
Structure Level Pattern Image Note
TS21: humerus cartilage condensation Present 3A Col2a1 11Prom-Cre; Sox9flox/flox
Specimen S2C Sox9flox/wt: embryonic day 15.5; Sox9tm2Crm/Sox9+ (more )
Note: Proximal humerus.
Structure Level Pattern Image Note
TS23: humerus Present S2C Col2a1 Sox9flox/+
Specimen S2C 11Enh-Cre; Sox9flox/flox: embryonic day 15.5; Sox9tm2Crm/Sox9tm2Crm, Tg(Col11a2-cre)1Ntsu/0 (more )
Note: Conditional mutant. Proximal humerus.
Structure Level Pattern Image Note
TS23: humerus Weak S2C Col2a1 11Enh-Cre;Sox9flox/flox Expression was almost completely lost in the conditional mutant.
Specimen S4A Sox9flox/+: embryonic day 14.5; Sox9tm2Crm/Sox9+ (more )
Note: Proximal humerus.
Structure Cell Type Level Pattern Image Note
TS22: humerus cartilage condensation chondrocyte Present S4A Col2a1 Sox9flox/+ Expression corresponded to that of Sox9 - which was detected in ephiphyseal round chondrocytes and metaphyseal flat chondrocytes.
Specimen S4A 11Prom-Cre; Sox9flox/flox: embryonic day 14.5; Sox9tm2Crm/Sox9tm2Crm, Tg(Col11a2-cre)2Ntsu/0 (more )
Note: Conditional mutant. Proximal humerus.
Structure Cell Type Level Pattern Image Note
TS22: humerus cartilage condensation chondrocyte Present Regionally restricted S4A Col2a1 11Prom-Cre;Sox9flox/flox Expression corresponded to that of Sox9 - which was only detected in ephiphyseal round chondrocytes in the conditional mutant.
Specimen S6 Sox9flox/+; Ptenflox/+: embryonic day 14.5; Ptentm2Mak/Pten+, Sox9tm2Crm/Sox9+ (more )
Structure Cell Type Level Pattern Image Note
TS22: humerus cartilage condensation chondrocyte Present Regionally restricted S6_Col2a1 Sox9flox/+;Ptenflox/+ Expression was detected in flat chondrocytes.
Specimen S6 11Prom-Cre; Sox9flox/flox; Pten+/+: embryonic day 14.5; Sox9tm2Crm/Sox9tm2Crm, Tg(Col11a2-cre)2Ntsu/0 (more )
Note: Conditional mutant.
Structure Cell Type Level Pattern Image Note
TS22: humerus cartilage condensation chondrocyte Weak Regionally restricted S6_Col2a1 11Prom-Cre;Sox9flox/flox;Pten+/+ Expression was decreased in proliferative chondrocytes.
Specimen S6 11Prom-Cre; Sox9flox/flox; Ptenflox/flox: embryonic day 14.5; Ptentm2Mak/Ptentm2Mak, Sox9tm2Crm/Sox9tm2Crm, Tg(Col11a2-cre)2Ntsu/0 (more )
Note: Conditional mutant.
Structure Cell Type Level Pattern Image Note
TS22: humerus cartilage condensation chondrocyte Weak Regionally restricted S6_Col2a1 11Prom-Cre;Sox9flox/flox;Ptenflox/flox Expression was decreased in proliferative chondrocytes.
Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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05/21/2024
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