About   Help   FAQ
Gene Expression Data
Assay Details
Assay
Reference: J:171508 Ikegami D, et al., Sox9 sustains chondrocyte survival and hypertrophy in part through Pik3ca-Akt pathways. Development. 2011 Apr;138(8):1507-19
Assay type: Immunohistochemistry
MGI Accession ID: MGI:7286330
Gene symbol: Sox9
Gene name: SRY (sex determining region Y)-box 9
Antibody: anti-Sox-9 (H-90; sc-20095)
Detection system: Secondary antibody coupled to Alexa Fluor 488
Results
Specimen 1A Sox9flox/+: embryonic day 12.5; Sox9tm2Crm/Sox9+ (more )
Structure Level Pattern Image Note
TS20: hand mesenchyme Present 1A anti-Sox9 Sox9flox/+
Specimen 1A 11Enh-Cre; Sox9flox/flox: embryonic day 12.5; Sox9tm2Crm/Sox9tm2Crm, Tg(Col11a2-cre)1Ntsu/0 (more )
Note: Conditional mutant.
Structure Level Pattern Image Note
TS20: hand Present 1A anti-Sox9 11Enh-Cre; Sox9flox/flox Sox9 expression pattern was normal in the paw.
Specimen 1B Sox9flox/+: embryonic day 12.5; Sox9tm2Crm/Sox9+ (more )
Structure Cell Type Level Pattern Image Note
TS20: humerus pre-cartilage condensation chondrocyte Present 1B anti-Sox9 Sox9flox/+
Specimen 1B 11Enh-Cre; Sox9flox/flox: embryonic day 12.5; Sox9tm2Crm/Sox9tm2Crm, Tg(Col11a2-cre)1Ntsu/0 (more )
Note: Conditional mutant.
Structure Cell Type Level Pattern Image Note
TS20: humerus pre-cartilage condensation chondrocyte Present Regionally restricted 1B anti-Sox9 11Enh-Cre; Sox9flox/flox Round chondrocytes in the central region of condensation in the conditional mutant humerus showed lost Sox9 expression.
Specimen 1C Sox9flox/+: embryonic day 13.5; Sox9tm2Crm/Sox9+ (more )
Structure Cell Type Level Pattern Image Note
TS21: humerus cartilage condensation chondrocyte Present 1C anti-Sox9 Sox9flox/+
Specimen 1C 11Enh-Cre; Sox9flox/flox: embryonic day 13.5; Sox9tm2Crm/Sox9tm2Crm, Tg(Col11a2-cre)1Ntsu/0 (more )
Note: Conditional mutant.
Structure Cell Type Level Pattern Image Note
TS21: humerus cartilage condensation chondrocyte Present Regionally restricted 1C anti-Sox9 11Enh-Cre; Sox9flox/flox Most chondrocytes in the conditional mutant proximal humerus did not express Sox9.
Specimen 1D Sox9flox/+: embryonic day 14.5; Sox9tm2Crm/Sox9+ (more )
Structure Cell Type Level Pattern Image Note
TS22: humerus cartilage condensation chondrocyte Present 1D anti-Sox9 Sox9flox/+
Specimen 1D 11Enh-Cre; Sox9flox/flox: embryonic day 14.5; Sox9tm2Crm/Sox9tm2Crm, Tg(Col11a2-cre)1Ntsu/0 (more )
Note: Conditional mutant.
Structure Cell Type Level Pattern Image Note
TS22: humerus cartilage condensation chondrocyte Ambiguous 1D anti-Sox9 11Enh-Cre; Sox9flox/flox
Specimen 2C Sox9flox/+: embryonic day 13.0; Sox9tm2Crm/Sox9+ (more )
Structure Cell Type Level Pattern Image Note
TS21: humerus cartilage condensation chondrocyte Present 2C Anti-Sox9 Sox9flox/+
Specimen 2C 11Prom-Cre; Sox9flox/flox: embryonic day 13.0; Sox9tm2Crm/Sox9tm2Crm, Tg(Col11a2-cre)2Ntsu/0 (more )
Note: Conditional mutant.
Structure Cell Type Level Pattern Image Note
TS21: humerus cartilage condensation chondrocyte Present 2C Anti-Sox9 11Prom-Cre; Sox9flox/flox Normal Sox9 expression was present at E13.0.
Specimen 2D Sox9flox/+: embryonic day 13.5; Sox9tm2Crm/Sox9+ (more )
Structure Cell Type Level Pattern Image Note
TS21: humerus cartilage condensation chondrocyte Present Regionally restricted 2D Anti-Sox9 Sox9flox/+ Expression was detected in proliferative chondrocytes. Expression was not detected in prehypertrophic chondrocytes.
Specimen 2D 11Prom-Cre; Sox9flox/flox: embryonic day 13.5; Sox9tm2Crm/Sox9tm2Crm, Tg(Col11a2-cre)2Ntsu/0 (more )
Note: Conditional mutant.
Structure Cell Type Level Pattern Image Note
TS21: humerus cartilage condensation chondrocyte Present Regionally restricted 2D Anti-Sox9 11Prom-Cre; Sox9flox/flox The length of the zone of proliferative chondrocytes expressing Sox9 was 25% shorter in the conditional mutant. Results suggest that Sox9 expression was lost in several layers of flat chondrocytes closest to the prehypertrophic chondrocytes.
Specimen 4G Sox9flox/+: embryonic day 13.5; Sox9tm2Crm/Sox9+ (more )
Structure Level Pattern Image Note
TS21: humerus cartilage condensation Present Regionally restricted 4G Anti-Sox9 Sox9flox/+ Expression was detected in the central and peripheral parts of primordial cartilage.
Specimen 4G 11Enh-Cre; Sox9flox/flox: embryonic day 13.5; Sox9tm2Crm/Sox9tm2Crm, Tg(Col11a2-cre)1Ntsu/0 (more )
Note: Conditional mutant.
Structure Level Pattern Image Note
TS21: humerus cartilage condensation Present Regionally restricted 4G Anti-Sox9 11Enh-Cre; Sox9flox/flox The area of Sox9 deletion was limited to the central part of cartilage in the conditional mutant.
Specimen 4H Sox9flox/+: embryonic day 14.5; Sox9tm2Crm/Sox9+ (more )
Structure Cell Type Level Pattern Image Note
TS22: humerus cartilage condensation chondrocyte Present 4H Anti-Sox9 Sox9flox/+ Expression was detected in epiphyseal round chondrocytes and metaphyseal flat proliferative chondrocytes.
Specimen 4H 11Prom-Cre; Sox9flox/flox: embryonic day 14.5; Sox9tm2Crm/Sox9tm2Crm, Tg(Col11a2-cre)2Ntsu/0 (more )
Note: Conditional mutant.
Structure Cell Type Level Pattern Image Note
TS22: humerus cartilage condensation chondrocyte Present Regionally restricted 4H Anti-Sox9 11Prom-Cre; Sox9flox/flox Sox9 was only detected in epiphyseal round chondrocytes. Metaphyseal flat proliferative chondrocytes did not express Sox9.
Specimen 5D Sox9flox/+; Ptenflox/+: embryonic day 14.5; Ptentm2Mak/Pten+, Sox9tm2Crm/Sox9+ (more )
Note: Metaphyseal region of the humerus.
Structure Cell Type Level Pattern Image Note
TS22: humerus cartilage condensation chondrocyte Present Regionally restricted 5D Anti-Sox9 Sox9flox/+;Ptenflox/+ Expression was in proliferative chondrocytes in the metaphyseal region.
Specimen 5D 11Prom-Cre; Sox9flox/flox; Pten+/+: embryonic day 14.5; Sox9tm2Crm/Sox9tm2Crm, Tg(Col11a2-cre)2Ntsu/0 (more )
Note: Conditional mutant. Metaphyseal region of the humerus.
Structure Cell Type Level Pattern Image Note
TS22: humerus cartilage condensation chondrocyte Ambiguous 5D Anti-Sox9 11Prom-Cre;Sox9flox/flox;Pten+/+ Metaphyseal chondrocytes do not express Sox9 in the conditional mutant.
Specimen 5D 11Prom-Cre; Sox9flox/flox; Ptenflox/flox: embryonic day 14.5; Ptentm2Mak/Ptentm2Mak, Sox9tm2Crm/Sox9tm2Crm, Tg(Col11a2-cre)2Ntsu/0 (more )
Note: Conditional mutant. Metaphyseal region of the humerus.
Structure Cell Type Level Pattern Image Note
TS22: humerus cartilage condensation chondrocyte Ambiguous 5D Anti-Sox9 11Prom-Cre;Sox9flox/flox;Ptenflox/flox Metaphyseal chondrocytes do not express Sox9 in the conditional mutant.
Specimen 6E Sox9flox/+: embryonic day 14.5; Sox9tm2Crm/Sox9+ (more )
Note: Proximal humerus.
Structure Cell Type Level Pattern Image Note
TS22: humerus cartilage condensation chondrocyte Present Regionally restricted 6E anti-Sox9 Sox9flox/+ Expression was detected in both round and flat proliferative chondrocytes.
Specimen 6E 11Prom-Cre; Sox9flox/flox: embryonic day 14.5; Sox9tm2Crm/Sox9tm2Crm, Tg(Col11a2-cre)2Ntsu/0 (more )
Note: Conditional mutant. Proximal humerus.
Structure Cell Type Level Pattern Image Note
TS22: humerus cartilage condensation chondrocyte Present Regionally restricted 6E anti-Sox9 11Prom-Cre; Sox9flox/flox In the conditional mutant, a line indicates chondrocytes in which Sox9 gene was deleted compared with Sox9-expressing chondrocytes.
Specimen S4A Sox9flox/+: embryonic day 14.5; Sox9tm2Crm/Sox9+ (more )
Note: Proximal humerus.
Structure Cell Type Level Pattern Image Note
TS22: humerus cartilage condensation chondrocyte Present Regionally restricted S4A Anti-Sox9 Sox9flox/+ Expression was detected in epiphyseal round chondrocytes and metaphyseal flat proliferative chondrocytes.
Specimen S4A 11Prom-Cre; Sox9flox/flox: embryonic day 14.5; Sox9tm2Crm/Sox9tm2Crm, Tg(Col11a2-cre)2Ntsu/0 (more )
Note: Conditional mutant. Proximal humerus.
Structure Cell Type Level Pattern Image Note
TS22: humerus cartilage condensation chondrocyte Present Regionally restricted S4A Anti-Sox9 11Prom-Cre;Sox9flox/flox Sox9 was only detected in epiphyseal round chondrocytes. Metaphyseal flat proliferative chondrocytes did not express Sox9.
Specimen S6 Sox9flox/+; Ptenflox/+: embryonic day 14.5; Ptentm2Mak/Pten+, Sox9tm2Crm/Sox9+ (more )
Structure Cell Type Level Pattern Image Note
TS22: humerus cartilage condensation chondrocyte Present Regionally restricted S6_Anti-Sox9 Sox9flox/+;Ptenflox/+ Expression was detected in round and flat proliferative chondrocytes.
Specimen S6 11Prom-Cre; Sox9flox/flox; Pten+/+: embryonic day 14.5; Sox9tm2Crm/Sox9tm2Crm, Tg(Col11a2-cre)2Ntsu/0 (more )
Note: Conditional mutant.
Structure Cell Type Level Pattern Image Note
TS22: humerus cartilage condensation chondrocyte Present Regionally restricted S6_Anti-Sox9 11Prom-Cre;Sox9flox/flox;Pten+/+ Expression was deleted in flat proliferative chondrocytes.
Specimen S6 11Prom-Cre; Sox9flox/flox; Ptenflox/flox: embryonic day 14.5; Ptentm2Mak/Ptentm2Mak, Sox9tm2Crm/Sox9tm2Crm, Tg(Col11a2-cre)2Ntsu/0 (more )
Note: Conditional mutant.
Structure Cell Type Level Pattern Image Note
TS22: humerus cartilage condensation chondrocyte Present Regionally restricted S6_Anti-Sox9 11Prom-Cre;Sox9flox/flox;Ptenflox/flox Expression was deleted in flat proliferative chondrocytes.
Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
Citing These Resources
Funding Information
Warranty Disclaimer, Privacy Notice, Licensing, & Copyright
Send questions and comments to User Support. 		last database update
05/21/2024
MGI 6.23 		The Jackson Laboratory