Gene Expression Data
Assay Details
Assay
|
Reference:
|
J:311536
Kim BJ, et al., RERE deficiency causes retinal and optic nerve atrophy through degeneration of retinal cells. Dev Dyn. 2021 Oct;250(10):1398-1409
|
Assay type:
|
Immunohistochemistry
|
MGI Accession ID:
|
MGI:7262882
|
Gene symbol:
|
Rere
|
Gene name:
|
arginine glutamic acid dipeptide (RE) repeats
|
|
Results
|
Specimen
1A:
embryonic day 13.5
(more )
Specimen
1A:
(close )
Genetic Background:
|
involves: 129/Sv * C57BL/6
|
Age:
|
embryonic day 13.5
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
Formaldehyde
|
Embedding:
|
Paraffin
|
Structure |
Level |
Pattern |
Image |
Note |
TS21: neural retina |
Present |
Regionally restricted |
1A'
,
1A
|
Expression was primarily detected in the ciliary margin of the neural retina. A few immunopositive cells were seen in the bottom of the ganglion cell layer. Expressing cells were not detected in the neuroblast layer.
|
TS21: lens epithelium |
Present |
|
1A
|
|
Specimen
1B:
embryonic day 15.5
(more )
Specimen
1B:
(close )
Genetic Background:
|
involves: 129/Sv * C57BL/6
|
Age:
|
embryonic day 15.5
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
Formaldehyde
|
Embedding:
|
Paraffin
|
Structure |
Level |
Pattern |
Image |
Note |
TS23: lens epithelium |
Present |
|
1B'
,
1B
|
|
TS23: retina layer |
Present |
Regionally restricted |
1B
,
1B"
|
Broad expression was detected in the ganglion cell layer. Expression in the ciliary margin of the neural retina disappeared at E15.5. Expressing cells were not detected in the neuroblast layer.
|
Specimen
1C:
embryonic day 17.5
(more )
Specimen
1C:
(close )
Genetic Background:
|
involves: 129/Sv * C57BL/6
|
Age:
|
embryonic day 17.5
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
Formaldehyde
|
Embedding:
|
Paraffin
|
Structure |
Level |
Pattern |
Image |
Note |
TS25: ciliary body |
Present |
|
1C'
,
1C
|
|
TS25: retina layer |
Present |
Regionally restricted |
1C
|
Expression was detected in the ganglion cell layer. A few immunopositive cells were seen in the junction between the neuroblast layer and inner plexiform layer.
|
Specimen
1D:
postnatal day 10
(more )
Specimen
1D:
(close )
Genetic Background:
|
involves: 129/Sv * C57BL/6
|
Age:
|
postnatal day 10
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
Formaldehyde
|
Embedding:
|
Paraffin
|
Specimen
1E:
embryonic day 17.5
(more )
Note: Double labeled: red - Rere; green - Pou4f2 (assay MGI:7262890).
Specimen
1E:
(close )
Genetic Background:
|
involves: 129/Sv * C57BL/6
|
Age:
|
embryonic day 17.5
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
Formaldehyde
|
Embedding:
|
Paraffin
|
Note:
|
Double labeled: red - Rere; green - Pou4f2 (assay MGI:7262890).
|
Structure |
Cell Type |
Level |
Pattern |
Image |
Note |
TS25: retina layer |
retinal ganglion cell |
Present |
Regionally restricted |
1E
,
1G
|
A subset of Pou4f2-positive retinal ganglion cells was also Rere-immunopositive.
|
TS25: retina layer |
|
Present |
Regionally restricted |
1G
,
1E
|
A few Rere-positive cells were also found in the junction between the neuroblast layer and the inner plexiform layer, which were not labeled with Pou4f2.
|
Specimen
2B Rere+/+:
embryonic day 17.5
(more )
Note: Double labeled: red - Rere; green - Pou4f2 (assay MGI:7262890).
Specimen
2B Rere<+/+>:
(close )
Genetic Background:
|
involves: 129S6/SvEvTac * BTBR * C57BL/6
|
Age:
|
embryonic day 17.5
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
Formaldehyde
|
Embedding:
|
Paraffin
|
Note:
|
Double labeled: red - Rere; green - Pou4f2 (assay MGI:7262890).
|
Specimen
2B Rereom/eyes3:
embryonic day 17.5; Rereeyes3/ Rereom
(more )
Note: Double labeled: red - Rere; green - Pou4f2 (assay MGI:7262890).
Specimen
2B Rere:
(close )
Genetic Background:
|
involves: 129S6/SvEvTac * BTBR * C57BL/6
|
Mutant Allele(s):
|
Rereeyes3/Rereom
|
Age:
|
embryonic day 17.5
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
Formaldehyde
|
Embedding:
|
Paraffin
|
Note:
|
Double labeled: red - Rere; green - Pou4f2 (assay MGI:7262890).
|
|