Gene Expression Data
Assay Details
Assay
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Reference:
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J:310857
Welcker D, et al., Hemicentin-1 is an essential extracellular matrix component of the dermal-epidermal and myotendinous junctions. Sci Rep. 2021 Sep 9;11(1):17926
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Assay type:
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Immunohistochemistry
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MGI Accession ID:
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MGI:6883743
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Gene symbol:
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Hmcn2
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Gene name:
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hemicentin 2
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Antibody:
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anti-Hmcn2
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Detection system:
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Secondary antibody coupled to Alexa Fluor 555
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Results
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Specimen
2B/B':
postnatal day 1
(more )
Note: Double labeled: red - Hmcn2; green - Tnc (assay MGI:6885592).
Specimen
2B/B':
(close )
Genetic Background:
|
Not Specified
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Age:
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postnatal day 1
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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Note:
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Double labeled: red - Hmcn2; green - Tnc (assay MGI:6885592).
|
Structure |
Level |
Pattern |
Image |
Note |
TS27: epidermis |
Present |
Regionally restricted |
2B'
,
2B
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Expressed in the pericellular space of basal keratinocytes.
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Specimen
2D/D':
postnatal adult
(more )
Note: Double labeled: red - Hmcn2; green - Tnc (assay MGI:6885592).
Specimen
2D/D':
(close )
Genetic Background:
|
Not Specified
|
Age:
|
postnatal adult
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Cryosection
|
Note:
|
Double labeled: red - Hmcn2; green - Tnc (assay MGI:6885592).
|
Structure |
Level |
Pattern |
Image |
Note |
TS28: epidermis |
Present |
Regionally restricted |
2D
,
2D'
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Expressed in basal keratinocytes of hair follicles and the interfollicular epidermis.
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Specimen
2F/F':
postnatal day 1
(more )
Note: Double labeled: red - Hmcn2; green - Tnc (assay MGI:6885592).
Specimen
2F/F':
(close )
Genetic Background:
|
Not Specified
|
Age:
|
postnatal day 1
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Cryosection
|
Note:
|
Double labeled: red - Hmcn2; green - Tnc (assay MGI:6885592).
|
Structure |
Level |
Pattern |
Image |
Note |
TS27: lower leg muscle |
Strong |
Regionally restricted |
2F
,
2F'
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Expressed in the endomysium of myofibers, with strong enrichment at the sites where myofibers were in close proximity to the tendon.
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Specimen
2H:
postnatal adult
(more )
Note: Double labeled: red - Hmcn2; green - Tnc (assay MGI:6885592).
Specimen
2H:
(close )
Genetic Background:
|
Not Specified
|
Age:
|
postnatal adult
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Cryosection
|
Note:
|
Double labeled: red - Hmcn2; green - Tnc (assay MGI:6885592).
|
Structure |
Level |
Pattern |
Image |
Note |
TS28: lower leg muscle |
Strong |
Regionally restricted |
2H
|
Expressed in the endomysium of myofibers, with strong enrichment at the sites where myofibers were in close proximity to the tendon.
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Specimen
2J/J':
postnatal day 1
(more )
Note: Double labeled: red - Hmcn2; green - Tnc (assay MGI:6885592).
Specimen
2J/J':
(close )
Genetic Background:
|
Not Specified
|
Age:
|
postnatal day 1
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Cryosection
|
Note:
|
Double labeled: red - Hmcn2; green - Tnc (assay MGI:6885592).
|
|