Shh RNA in situ Gene Expression Assay

Gene Expression Data
Assay Details

Assay

Reference:	J:314133 Marczenke M, et al., GAS1 is required for NOTCH-dependent facilitation of SHH signaling in the ventral forebrain neuroepithelium. Development. 2021 Nov 1;148(21):dev200080
Assay type:	RNA in situ
MGI Accession ID:	MGI:6866988
Gene symbol:	Shh
Gene name:	sonic hedgehog

Probe:	Mm-Shh (314361)
Probe preparation:	Antisense
Visualized with:	Fluorescein

Results

1A Shh Control ©

Specimen 1A Shh Control: embryonic day 8.5 (more

)
Note: 8-9 somites

Structure	Level	Pattern	Image	Note
TS13: future prosencephalon	Present	Regionally restricted	1A Shh Control	Expression in prechordal plate (PrCP) and overlying rostral diencephalon ventral midline (RDVM).

1A Shh Gas1-/- ©

Specimen 1A Shh Gas1-/-: embryonic day 8.5; Gas1^tm2Fan/Gas1^tm2Fan (more

)
Note: 8-9 somites

Structure	Level	Pattern	Image	Note
TS13: future prosencephalon	Present	Regionally restricted	1A Shh Gas1-/-	No difference in expression compared to control.

1B Shh Control ©

Specimen 1B Shh Control: embryonic day 8.5 (more

)
Note: 10-11 somites

Structure	Level	Pattern	Image	Note
TS13: future prosencephalon	Present	Regionally restricted	1B Shh Control	Expression in prechordal plate (PrCP) and overlying rostral diencephalon ventral midline (RDVM).

1B Shh Gas1-/- ©

Specimen 1B Shh Gas1-/-: embryonic day 8.5; Gas1^tm2Fan/Gas1^tm2Fan (more

)
Note: 10-11 somites

Structure	Level	Pattern	Image	Note
TS13: future prosencephalon	Present	Regionally restricted	1B Shh Gas1-/-	No difference in expression compared to control.

S1A 8-9 somites Control merge ©

S1A 8-9 somites Shh Control ©

Specimen S1A 8-9 somites Shh Control: embryonic day 8.5 (more

)
Note: 8-9 somites. Triple labeled: red - Shh RNA; magenta - SHH protein (assay MGI:6866996); green - Gli1 RNA (assay MGI:6866989).

Structure	Level	Pattern	Image	Note
TS13: future prosencephalon	Present	Regionally restricted	S1A 8-9 somites Control merge , S1A 8-9 somites Shh Control	Expression in prechordal plate (PrCP) and overlying rostral diencephalon ventral midline (RDVM). Co-expression with SHH protein and Gli1.

S1A 8-9 somites Gas1-/- merge ©

S1A 8-9 somites Shh Gas1-/- ©

Specimen S1A 8-9 somites Shh Gas1-/-: embryonic day 8.5; Gas1^tm2Fan/Gas1^tm2Fan (more

)
Note: 8-9 somites. Triple labeled: red - Shh RNA; magenta - SHH protein (assay MGI:6866996); green - Gli1 RNA (assay MGI:6866989).

Structure	Level	Pattern	Image	Note
TS13: future prosencephalon	Present	Regionally restricted	S1A 8-9 somites Shh Gas1-/- , S1A 8-9 somites Gas1-/- merge	No difference in size of expression compared to control.

S1A 10-11 somites Control merge ©

S1A 10-11 somites Shh Control ©

Specimen S1A 10-11 somites Shh Control: embryonic day 8.5 (more

)
Note: 10-11 somites. Triple labeled: red - Shh RNA; magenta - SHH protein (assay MGI:6866996); green - Gli1 RNA (assay MGI:6866989).

Structure	Level	Pattern	Image	Note
TS13: future prosencephalon	Present	Regionally restricted	S1A 10-11 somites Shh Control , S1A 10-11 somites Control merge	Expression in prechordal plate (PrCP) and overlying rostral diencephalon ventral midline (RDVM). Co-expression with SHH protein and Gli1.

S1A 10-11 somites Gas1-/- merge ©

S1A 10-11 somites Shh Gas1-/- ©

Specimen S1A 10-11 somites Shh Gas1-/-: embryonic day 8.5; Gas1^tm2Fan/Gas1^tm2Fan (more

)
Note: 10-11 somites. Triple labeled: red - Shh RNA; magenta - SHH protein (assay MGI:6866996); green - Gli1 RNA (assay MGI:6866989).

Structure	Level	Pattern	Image	Note
TS13: future prosencephalon	Present	Regionally restricted	S1A 10-11 somites Shh Gas1-/- , S1A 10-11 somites Gas1-/- merge	No difference in size of expression compared to control.

S1B Control ©

Specimen S1B Control: embryonic day 8.5 (more

)

Structure	Level	Pattern	Image	Note
TS13: future prosencephalon	Present	Regionally restricted	S1B Control	Expression in prechordal plate (PrCP) and overlying rostral diencephalon ventral midline (RDVM).

S1B Gas1-/- ©

Specimen S1B Gas1-/-: embryonic day 8.5; Gas1^tm2Fan/Gas1^tm2Fan (more

)

Structure	Level	Pattern	Image	Note
TS13: future prosencephalon	Present	Regionally restricted	S1B Gas1-/-	No difference in size of expression compared to control.

Specimen 1C Shh Control: embryonic day 9.5 (more

)

Structure	Level	Pattern	Image	Note
TS15: future forebrain	Present	Regionally restricted	1C Shh Control	Expression in rostral ventral neuroepithelium.

Specimen 1C Shh Gas1-/-: embryonic day 9.5; Gas1^tm2Fan/Gas1^tm2Fan (more

)

Structure	Level	Pattern	Image	Note
TS15: future forebrain	Weak	Regionally restricted	1C Shh Gas1-/-	Expression in rostral ventral neuroepithelium decreased compared to controls.

Specimen 1D Shh Control: embryonic day 10.5 (more

)

Structure	Level	Pattern	Image	Note
TS17: forebrain	Present	Regionally restricted	1D Shh Control	Expression in rostral ventral neuroepithelium.

Specimen 1D Shh Gas1-/-: embryonic day 10.5; Gas1^tm2Fan/Gas1^tm2Fan (more

)

Structure	Level	Pattern	Image	Note
TS17: forebrain	Absent		1D Shh Gas1-/-	Expression in rostral ventral neuroepithelium completely lost.

Contributing Projects: Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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