About   Help   FAQ
Gene Expression Data
Assay Details
Assay
Reference: J:314133 Marczenke M, et al., GAS1 is required for NOTCH-dependent facilitation of SHH signaling in the ventral forebrain neuroepithelium. Development. 2021 Nov 1;148(21):dev200080
Assay type: RNA in situ
MGI Accession ID: MGI:6866989
Gene symbol: Gli1
Gene name: GLI-Kruppel family member GLI1
Probe: Mm-Gli1 (311001)
Probe preparation: Antisense
Visualized with: Fluorescein
Results
Specimen 1A Gli1 Control: embryonic day 8.5 (more )
Note: 8-9 somites
Structure Level Pattern Image Note
TS13: future prosencephalon Present Regionally restricted 1A Gli1 Control Expression in prechordal plate (PrCP) and overlying rostral diencephalon ventral midline (RDVM).
Specimen 1A Gli1 Gas1-/-: embryonic day 8.5; Gas1tm2Fan/Gas1tm2Fan (more )
Note: 8-9 somites
Structure Level Pattern Image Note
TS13: future prosencephalon Present Regionally restricted 1A Gli1 Gas1-/- No difference in expression compared to control
Specimen 1B Gli1 Control: embryonic day 8.5 (more )
Note: 10-11 somites
Structure Level Pattern Image Note
TS13: future prosencephalon Present Regionally restricted 1B Gli1 Control Expression in prechordal plate (PrCP) and overlying rostral diencephalon ventral midline (RDVM).
Specimen 1B Gli1 Gas1-/-: embryonic day 8.5; Gas1tm2Fan/Gas1tm2Fan (more )
Note: 10-11 somites
Structure Level Pattern Image Note
TS13: future prosencephalon Present Regionally restricted 1B Gli1 Gas1-/- No difference in expression compared to control.
Specimen S1A 8-9 somites Gli1h Control: embryonic day 8.5 (more )
Note: 8-9 somites. Triple labeled: green - Gli1 RNA; red - Shh RNA (assay MGI:6866988); magenta - SHH protein (assay MGI:6866996).
Structure Level Pattern Image Note
TS13: future prosencephalon Present Regionally restricted S1A 8-9 somites Control merge , S1A 8-9 somites Gli1 Control Expression in prechordal plate (PrCP) and overlying rostral diencephalon ventral midline (RDVM). Co-expression with Shh.
Specimen S1A 8-9 somites Gli1 Gas1-/-: embryonic day 8.5; Gas1tm2Fan/Gas1tm2Fan (more )
Note: 8-9 somites. Triple labeled: green - Gli1 RNA; red - Shh RNA (assay MGI:6866988); magenta - SHH protein (assay MGI:6866996).
Structure Level Pattern Image Note
TS13: future prosencephalon Present Regionally restricted S1A 8-9 somites Gas1-/- merge , S1A 8-9 somites Gli1 Gas1-/- No difference in expression compared to control.
Specimen S1A 10-11 somites Gli1 Control: embryonic day 8.5 (more )
Note: 10-11 somites. Triple labeled: green - Gli1 RNA; red - Shh RNA (assay MGI:6866988); magenta - SHH protein (assay MGI:6866996).
Structure Level Pattern Image Note
TS13: future prosencephalon Present Regionally restricted S1A 10-11 somites Gli1 Control , S1A 10-11 somites Control merge Expression in prechordal plate (PrCP) and overlying rostral diencephalon ventral midline (RDVM). Co-expression with Shh.
Specimen S1A 10-11 somites Gli1 Gas1-/-: embryonic day 8.5; Gas1tm2Fan/Gas1tm2Fan (more )
Note: 10-11 somites. Triple labeled: green - Gli1 RNA; red - Shh RNA (assay MGI:6866988); magenta - SHH protein (assay MGI:6866996).
Structure Level Pattern Image Note
TS13: future prosencephalon Present Regionally restricted S1A 10-11 somites Gli1 Gas1-/- , S1A 10-11 somites Gas1-/- merge No difference in expression compared to control.
Specimen 1C Gli1 Control: embryonic day 9.5 (more )
Structure Level Pattern Image Note
TS15: future forebrain Present Regionally restricted 1C Gli1 Control Expression in rostral ventral neuroepithelium.
Specimen 1C Gli1 Gas1-/-: embryonic day 9.5; Gas1tm2Fan/Gas1tm2Fan (more )
Structure Level Pattern Image Note
TS15: future forebrain Weak Regionally restricted 1C Gli1 Gas1-/- Expression in rostral ventral neuroepithelium decreased compared to controls.
Specimen 1D Gli1 Control: embryonic day 10.5 (more )
Structure Level Pattern Image Note
TS17: forebrain Present Regionally restricted 1D Gli1 Control Expression in rostral ventral neuroepithelium
Specimen 1D Gli1 Gas1-/-: embryonic day 10.5; Gas1tm2Fan/Gas1tm2Fan (more )
Structure Level Pattern Image Note
TS17: forebrain Absent 1D Gli1 Gas1-/- Expression in rostral ventral neuroepithelium completely lost.
Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
Citing These Resources
Funding Information
Warranty Disclaimer, Privacy Notice, Licensing, & Copyright
Send questions and comments to User Support. 		last database update
03/25/2025
MGI 6.24 		The Jackson Laboratory