Probe:	Emilin3 cDNA2
Probe preparation:	Antisense labelled with digoxigenin RNA

Structure	Level	Pattern	Image	Note
TS19: midbrain ventricular layer	Present	Regionally restricted	3A	Expression is ventral.
TS19: diencephalon ventricular layer	Present		3A
TS19: gut	Present	Regionally restricted	3A	Expression is diffuse in the wall of the gastrointestinal system from the esophagus to the hindgut.
TS19: cardiovascular system	Absent		3A

Structure	Level	Pattern	Image	Note
TS19: midbrain ventricular layer	Present	Regionally restricted	3B	Expression is ventral.

Structure	Level	Pattern	Image	Note
TS19: midbrain ventricular layer	Present	Regionally restricted	3C	Expression is ventral.

Structure	Level	Pattern	Image	Note
TS20: esophagus	Present	Regionally restricted	3D	Expression is diffuse in the wall of the gastrointestinal system from the esophagus to the hindgut.
TS20: gut	Present	Regionally restricted	3D	Expression is diffuse in the wall of the gastrointestinal system from the esophagus to the hindgut.

Structure	Level	Pattern	Image	Note
TS22: enteric nervous system	Present	Regionally restricted	3E	Expression is in a thin layer corresponding to the myenteric plexus, showing a craniocaudal gradient with strongest signal in the esophagus.
TS22: esophagus	Present	Regionally restricted	3E	Expression is in a thin layer corresponding to the myenteric plexus, showing a craniocaudal gradient with strongest signal in the esophagus.
TS22: gut	Present	Regionally restricted	3E	Expression is in a thin layer corresponding to the myenteric plexus, showing a craniocaudal gradient with strongest signal in the esophagus.

Structure	Level	Pattern	Image	Note
TS19: enteric nervous system	Present	Regionally restricted	3F	Expression is in a thin layer corresponding to the myenteric plexus, showing a craniocaudal gradient with strongest signal in the esophagus (shown midgut).
TS19: esophagus	Present	Regionally restricted	3F	Expression is in a thin layer corresponding to the myenteric plexus, showing a craniocaudal gradient with strongest signal in the esophagus (shown midgut).
TS19: gut	Present	Regionally restricted	3F	Expression is in a thin layer corresponding to the myenteric plexus, showing a craniocaudal gradient with strongest signal in the esophagus (shown midgut).
TS19: midgut	Present	Regionally restricted	3F	Expression is in a thin layer corresponding to the myenteric plexus, showing a craniocaudal gradient with strongest signal in the esophagus (shown midgut).

Structure	Level	Pattern	Image	Note
TS20: enteric nervous system	Present	Regionally restricted	3G	Expression is in a thin layer corresponding to the myenteric plexus, showing a craniocaudal gradient with strongest signal in the esophagus (shown midgut).
TS20: esophagus	Present	Regionally restricted	3G	Expression is in a thin layer corresponding to the myenteric plexus, showing a craniocaudal gradient with strongest signal in the esophagus (shown midgut).
TS20: gut	Present	Regionally restricted	3G	Expression is in a thin layer corresponding to the myenteric plexus, showing a craniocaudal gradient with strongest signal in the esophagus (shown midgut).
TS20: midgut	Present	Regionally restricted	3G	Expression is in a thin layer corresponding to the myenteric plexus, showing a craniocaudal gradient with strongest signal in the esophagus (shown midgut).

Structure	Level	Pattern	Image	Note
TS22: enteric nervous system	Present	Regionally restricted	3H	Expression is in a thin layer corresponding to the myenteric plexus, showing a craniocaudal gradient with strongest signal in the esophagus (shown midgut).
TS22: esophagus	Present	Regionally restricted	3H	Expression is in a thin layer corresponding to the myenteric plexus, showing a craniocaudal gradient with strongest signal in the esophagus (shown midgut).
TS22: gut	Present	Regionally restricted	3H	Expression is in a thin layer corresponding to the myenteric plexus, showing a craniocaudal gradient with strongest signal in the esophagus (shown midgut).
TS22: midgut	Present	Regionally restricted	3H	Expression is in a thin layer corresponding to the myenteric plexus, showing a craniocaudal gradient with strongest signal in the esophagus (shown midgut).

Structure	Level	Pattern	Image	Note
TS21: integumental system mesenchyme	Strong	Regionally restricted	3I	Expression is in the subepidermal mesenchyme.
TS21: trunk skin	Strong	Regionally restricted	3I	Expression is in the subepidermal mesenchyme.

Structure	Level	Pattern	Image	Note
TS20: urogenital ridge	Strong		3J
TS20: metanephros	Absent		3J

Structure	Level	Pattern	Image	Note
TS21: urogenital ridge	Strong		3K
TS21: metanephros	Absent		3K

Structure	Level	Pattern	Image	Note
TS22: mesonephros	Strong		3L
TS22: testis	Strong		3L
TS22: metanephros	Absent		3L

Structure	Level	Pattern	Image	Note
TS22: diaphragm	Present		3M
TS22: head mesenchyme	Present		3M
TS22: intervertebral disc	Present		3M
TS22: lung	Present	Regionally restricted	3M	Expression is in the region surrounding the main branches of alveoli.
TS22: mystacial pad	Present	Regionally restricted	3M	Expression is in the mesenchymal tissue around vibrissae.
TS22: hindlimb	Present	Regionally restricted	3M	Expression is in the perichondrium of the hindlimb.
TS22: perichondrium	Present	Regionally restricted	3M	Expression is in the perichondrium of the hindlimb.
TS22: cardiovascular system	Absent		3M

Structure	Level	Pattern	Image	Note
TS22: eye	Present		3N

Structure	Level	Pattern	Image	Note
TS22: mystacial pad	Present	Regionally restricted	3O	Expression is in the mesenchymal tissue around vibrissae.

Structure	Level	Pattern	Image	Note
TS22: diaphragm	Present		3P
TS22: lung	Present	Regionally restricted	3P	Expression is in the region surrounding the main branches of alveoli.

Structure	Level	Pattern	Image	Note
TS22: intervertebral disc	Present		3Q

Structure	Level	Pattern	Image	Note
TS22: perichondrium	Strong		3R
TS22: hindlimb	Strong	Regionally restricted	3R	Expression is in the perichondrium of the hindlimb.

Structure	Level	Pattern	Image	Note
TS22: inner ear	Present		3S	Expression is in the perichondrium around sites of bone and cartilage formation.

Structure	Level	Pattern	Image	Note
TS13: trunk	Weak	Regionally restricted	S3A 8.5	Expression is in the tail bud.
TS13: cardiovascular system	Absent		S3A 8.5

Structure	Level	Pattern	Image	Note
TS15: tail	Present		S3A 9.5
TS15: hindgut	Weak		S3A 9.5
TS15: cardiovascular system	Absent		S3A 9.5

Structure	Level	Pattern	Image	Note
TS19: diencephalon ventricular layer	Present		S3B 11.5
TS19: midbrain ventricular layer	Present		S3B 11.5
TS19: cardiovascular system	Absent		S3B 11.5

Structure	Level	Pattern	Image	Note
TS22: diaphragm	Present		S3B 14.5
TS22: head mesenchyme	Present		S3B 14.5
TS22: intervertebral disc	Present		S3B 14.5
TS22: lung	Present	Regionally restricted	S3B 14.5	Expression is in the region surrounding the main branches of alveoli.
TS22: mystacial pad	Present	Regionally restricted	S3B 14.5	Expression is in the mesenchymal tissue around vibrissae.
TS22: hindlimb	Present	Regionally restricted	S3B 14.5	Expression is in the perichondrium of the hindlimb.
TS22: perichondrium	Present	Regionally restricted	S3B 14.5	Expression is in the perichondrium of the hindlimb.
TS22: cardiovascular system	Absent		S3B 14.5

Structure	Level	Pattern	Image	Note
TS22: heart	Absent		S3C left

Structure	Level	Pattern	Image	Note
TS22: blood vessel	Absent		S3C right

Structure	Level	Pattern	Image	Note
TS17: branchial arch mesenchyme	Strong
TS17: esophagus	Strong
TS17: tail	Strong
TS17: midbrain	Strong	Regionally restricted		Expression is in a small region.

Structure	Level	Pattern	Image	Note
TS20: diencephalon ventricular layer	Present

Structure	Level	Pattern	Image	Note
TS21: diencephalon ventricular layer	Absent