Gene Expression Data
Assay Details
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Assay
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Reference:
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J:142507
Tian NM, et al., Foxg1 regulates retinal axon pathfinding by repressing an ipsilateral program in nasal retina and by causing optic chiasm cells to exert a net axonal growth-promoting activity. Development. 2008 Dec;135(24):4081-9
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Assay type:
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Immunohistochemistry
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MGI Accession ID:
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MGI:6842542
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Gene symbol:
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Zic2
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Gene name:
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zinc finger protein of the cerebellum 2
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Antibody:
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anti-Zic2
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Detection system:
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Secondary antibody coupled to Alexa Fluor 488
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Results
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Specimen
2A:
embryonic day 14.5
(more  )
Note: Triple labeled: green - Zic2; red - Brn3a (Pou4f1 assay MGI:6842543); blue - TOPRO-3.
Specimen
2A:
(close  )
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Genetic Background:
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Not Specified
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Age:
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embryonic day 14.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Note:
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Triple labeled: green - Zic2; red - Brn3a (Pou4f1 assay MGI:6842543); blue - TOPRO-3.
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| Structure |
Level |
Pattern |
Image |
Note |
| TS22: retina |
Present |
Regionally restricted |
2A
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Few expressing cells in retinal ganglion cell (RGC) layer; some seen in other layers.
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Specimen
2B:
embryonic day 14.5
(more )
Note: Triple labeled: green - Zic2; red - Brn3a (Pou4f1 assay MGI:6842543); blue - TOPRO-3.
Specimen
2B:
(close )
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Genetic Background:
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Not Specified
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Age:
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embryonic day 14.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Note:
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Triple labeled: green - Zic2; red - Brn3a (Pou4f1 assay MGI:6842543); blue - TOPRO-3.
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| Structure |
Level |
Pattern |
Image |
Note |
| TS22: retina |
Present |
Regionally restricted |
2B
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Few expressing cells in retinal ganglion cell (RGC) layer; some seen in other layers.
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Specimen
2C:
embryonic day 14.5
(more )
Note: Triple labeled: green - Zic2; red - Brn3a (Pou4f1 assay MGI:6842543); blue - TOPRO-3.
Specimen
2C:
(close )
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Genetic Background:
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Not Specified
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Age:
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embryonic day 14.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Note:
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Triple labeled: green - Zic2; red - Brn3a (Pou4f1 assay MGI:6842543); blue - TOPRO-3.
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| Structure |
Level |
Pattern |
Image |
Note |
| TS22: retina |
Strong |
Regionally restricted |
2C
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Expressing cells in inner retinal layer, predominantly in ventral-temporal retina. Strongest expression in cluster of cells adjacent to ciliary marginal zone (CMZ).
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Specimen
2D:
embryonic day 14.5
(more )
Note: Triple labeled: green - Zic2; red - Brn3a (Pou4f1 assay MGI:6842543); blue - TOPRO-3.
Specimen
2D:
(close )
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Genetic Background:
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Not Specified
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Age:
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embryonic day 14.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Note:
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Triple labeled: green - Zic2; red - Brn3a (Pou4f1 assay MGI:6842543); blue - TOPRO-3.
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| Structure |
Level |
Pattern |
Image |
Note |
| TS22: retina |
Strong |
Regionally restricted |
2D
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Expressing cells in inner retinal layer, predominantly in ventral-temporal retina. Strongest expression in cluster of cells adjacent to ciliary marginal zone (CMZ).
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Specimen
2E:
embryonic day 14.5
(more )
Note: Triple labeled: green - Zic2; red - Brn3a (Pou4f1 assay MGI:6842543); blue - TOPRO-3.
Specimen
2E:
(close )
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Genetic Background:
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Not Specified
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Age:
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embryonic day 14.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Note:
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Triple labeled: green - Zic2; red - Brn3a (Pou4f1 assay MGI:6842543); blue - TOPRO-3.
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| Structure |
Level |
Pattern |
Image |
Note |
| TS22: retina |
Present |
Regionally restricted |
2E
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Few expressing cells in retinal ganglion cell (RGC) layer; some seen in other layers. Most expressing cell are outside the RGC layer.
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Specimen
2F:
embryonic day 14.5
(more )
Note: Triple labeled: green - Zic2; red - Brn3a (Pou4f1 assay MGI:6842543); blue - TOPRO-3.
Specimen
2F:
(close )
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Genetic Background:
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Not Specified
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Age:
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embryonic day 14.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Note:
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Triple labeled: green - Zic2; red - Brn3a (Pou4f1 assay MGI:6842543); blue - TOPRO-3.
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| Structure |
Level |
Pattern |
Image |
Note |
| TS22: retina |
Strong |
Regionally restricted |
2F
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Strong expression in ciliary marginal zone (CMZ). Most expressing cell are outside the RGC layer.
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Specimen
2G:
embryonic day 14.5
(more )
Note: Triple labeled: green - Zic2; red - Brn3a (Pou4f1 assay MGI:6842543); blue - TOPRO-3.
Specimen
2G:
(close )
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Genetic Background:
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Not Specified
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Age:
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embryonic day 14.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Note:
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Triple labeled: green - Zic2; red - Brn3a (Pou4f1 assay MGI:6842543); blue - TOPRO-3.
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| Structure |
Level |
Pattern |
Image |
Note |
| TS22: retina |
Strong |
Regionally restricted |
2G
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Expressing cells in inner retinal layer. Strongest expression in cluster of cells adjacent to ciliary marginal zone (CMZ).
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Specimen
2H:
embryonic day 14.5
(more )
Note: Triple labeled: green - Zic2; red - Brn3a (Pou4f1 assay MGI:6842543); blue - TOPRO-3.
Specimen
2H:
(close )
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Genetic Background:
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Not Specified
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Age:
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embryonic day 14.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Note:
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Triple labeled: green - Zic2; red - Brn3a (Pou4f1 assay MGI:6842543); blue - TOPRO-3.
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| Structure |
Level |
Pattern |
Image |
Note |
| TS22: retina |
Present |
Regionally restricted |
2H
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Most expressing cell are outside the RGC layer.
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Specimen
2I:
embryonic day 14.5; Foxg1tm1M/ Foxg1tm1M
(more )
Note: Triple labeled: green - Zic2; red - Brn3a (Pou4f1 assay MGI:6842543); blue - TOPRO-3.
Specimen
2I:
(close )
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Genetic Background:
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involves: 129S1/Sv * CBA
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Mutant Allele(s):
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Foxg1tm1M/Foxg1tm1M
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Age:
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embryonic day 14.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Note:
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Triple labeled: green - Zic2; red - Brn3a (Pou4f1 assay MGI:6842543); blue - TOPRO-3.
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| Structure |
Level |
Pattern |
Image |
Note |
| TS22: retina |
Present |
Regionally restricted |
2I
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Expression in cells in retinal ganglion cell (RGC) layer both nasally and temporally.
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Specimen
2J:
embryonic day 14.5; Foxg1tm1M/ Foxg1tm1M
(more )
Note: Triple labeled: green - Zic2; red - Brn3a (Pou4f1 assay MGI:6842543); blue - TOPRO-3.
Specimen
2J:
(close )
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Genetic Background:
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involves: 129S1/Sv * CBA
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Mutant Allele(s):
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Foxg1tm1M/Foxg1tm1M
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Age:
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embryonic day 14.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Note:
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Triple labeled: green - Zic2; red - Brn3a (Pou4f1 assay MGI:6842543); blue - TOPRO-3.
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| Structure |
Level |
Pattern |
Image |
Note |
| TS22: retina |
Present |
Regionally restricted |
2J
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Large increase in proportion of expressing cell in retinal ganglion cell (RGC)
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Specimen
2K:
embryonic day 14.5; Foxg1tm1M/ Foxg1tm1M
(more )
Note: Triple labeled: green - Zic2; red - Brn3a (Pou4f1 assay MGI:6842543); blue - TOPRO-3.
Specimen
2K:
(close )
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Genetic Background:
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involves: 129S1/Sv * CBA
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Mutant Allele(s):
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Foxg1tm1M/Foxg1tm1M
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Age:
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embryonic day 14.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Note:
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Triple labeled: green - Zic2; red - Brn3a (Pou4f1 assay MGI:6842543); blue - TOPRO-3.
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| Structure |
Level |
Pattern |
Image |
Note |
| TS22: retina |
Present |
Regionally restricted |
2K
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Large increase in proportion of expressing cell in retinal ganglion cell (RGC)
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Specimen
2L:
embryonic day 14.5; Foxg1tm1M/ Foxg1tm1M
(more )
Note: Triple labeled: green - Zic2; red - Brn3a (Pou4f1 assay MGI:6842543); blue - TOPRO-3.
Specimen
2L:
(close )
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Genetic Background:
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involves: 129S1/Sv * CBA
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Mutant Allele(s):
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Foxg1tm1M/Foxg1tm1M
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Age:
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embryonic day 14.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Note:
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Triple labeled: green - Zic2; red - Brn3a (Pou4f1 assay MGI:6842543); blue - TOPRO-3.
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| Structure |
Level |
Pattern |
Image |
Note |
| TS22: retina |
Present |
Regionally restricted |
2L
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Expression in cells in retinal ganglion cell (RGC) layer both nasally and temporally.
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Specimen
2M:
embryonic day 14.5; Foxg1tm1M/ Foxg1tm1M
(more )
Note: Triple labeled: green - Zic2; red - Brn3a (Pou4f1 assay MGI:6842543); blue - TOPRO-3.
Specimen
2M:
(close )
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Genetic Background:
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involves: 129S1/Sv * CBA
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Mutant Allele(s):
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Foxg1tm1M/Foxg1tm1M
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Age:
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embryonic day 14.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Note:
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Triple labeled: green - Zic2; red - Brn3a (Pou4f1 assay MGI:6842543); blue - TOPRO-3.
|
| Structure |
Level |
Pattern |
Image |
Note |
| TS22: retina |
Strong |
Regionally restricted |
2M
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Strong expression in ciliary marginal zone (CMZ).
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Specimen
2N:
embryonic day 14.5; Foxg1tm1M/ Foxg1tm1M
(more )
Note: Triple labeled: green - Zic2; red - Brn3a (Pou4f1 assay MGI:6842543); blue - TOPRO-3.
Specimen
2N:
(close )
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Genetic Background:
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involves: 129S1/Sv * CBA
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Mutant Allele(s):
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Foxg1tm1M/Foxg1tm1M
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Age:
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embryonic day 14.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Note:
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Triple labeled: green - Zic2; red - Brn3a (Pou4f1 assay MGI:6842543); blue - TOPRO-3.
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| Structure |
Level |
Pattern |
Image |
Note |
| TS22: retina |
Present |
Regionally restricted |
2N
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Large increase in proportion of expressing cell in retinal ganglion cell (RGC)
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Specimen
2O:
embryonic day 14.5; Foxg1tm1M/ Foxg1tm1M
(more )
Note: Triple labeled: green - Zic2; red - Brn3a (Pou4f1 assay MGI:6842543); blue - TOPRO-3.
Specimen
2O:
(close )
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Genetic Background:
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involves: 129S1/Sv * CBA
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Mutant Allele(s):
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Foxg1tm1M/Foxg1tm1M
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Age:
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embryonic day 14.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Note:
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Triple labeled: green - Zic2; red - Brn3a (Pou4f1 assay MGI:6842543); blue - TOPRO-3.
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| Structure |
Level |
Pattern |
Image |
Note |
| TS22: retina |
Present |
Regionally restricted |
2O
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Large increase in proportion of expressing cell in retinal ganglion cell (RGC)
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Specimen
2P:
embryonic day 14.5; Foxg1tm1M/ Foxg1tm1M
(more )
Note: Triple labeled: green - Zic2; red - Brn3a (Pou4f1 assay MGI:6842543); blue - TOPRO-3.
Specimen
2P:
(close )
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Genetic Background:
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involves: 129S1/Sv * CBA
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Mutant Allele(s):
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Foxg1tm1M/Foxg1tm1M
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Age:
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embryonic day 14.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Note:
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Triple labeled: green - Zic2; red - Brn3a (Pou4f1 assay MGI:6842543); blue - TOPRO-3.
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| Structure |
Level |
Pattern |
Image |
Note |
| TS22: retina |
Present |
Regionally restricted |
2P
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Expression in cells in retinal ganglion cell (RGC) layer both nasally and temporally.
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