Gene Expression Data
Assay Details
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Assay
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Reference:
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J:281028
Kiyama T, et al., Essential Roles of Tbr1 in the Formation and Maintenance of the Orientation-Selective J-RGCs and a Group of OFF-Sustained RGCs in Mouse. Cell Rep. 2019 Apr 16;27(3):900-915.e5
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Assay type:
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In situ reporter (knock in)
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MGI Accession ID:
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MGI:6781824
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Gene symbol:
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Tbr1
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Gene name:
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T-box brain transcription factor 1
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Reporter:
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GFP
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Antibody:
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anti-GFP (#A10262)
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Detection system:
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Biotinylated secondary antibody/[Strept]avidin coupled to Alexa Fluor 488
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Results
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Specimen
5B - E14.5:
(close  )
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Genetic Background:
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Not Specified
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Mutant Allele(s):
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Tbr1tm1.1(cre/ERT2)Maoc/Tbr1+
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Age:
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embryonic day 14.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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| Structure |
Level |
Pattern |
Image |
Note |
| TS22: neural retina |
Present |
Single cells |
5B E14.5
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Sparce numbers of positive cells seen in the neural blast layer and ganglion cell layer.
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Specimen
5B - E17.5:
(close )
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Genetic Background:
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Not Specified
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Mutant Allele(s):
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Tbr1tm1.1(cre/ERT2)Maoc/Tbr1+
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Age:
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embryonic day 17.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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| Structure |
Level |
Pattern |
Image |
Note |
| TS25: retina |
Present |
Single cells |
5B E17.5
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Sparce numbers of positive cells seen in the neural blast layer and ganglion cell layer.
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Specimen
6E:
(close )
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Genetic Background:
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Not Specified
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Mutant Allele(s):
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Tbr1tm1.1(cre/ERT2)Maoc/Tbr1+
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Age:
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postnatal day 30
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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4% Paraformaldehyde
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Specimen
6F:
(close )
| Structure |
Level |
Pattern |
Image |
Note |
| TS28: retina |
Trace |
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6F
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Expression is almost absent in the mutant.
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)
Analysis Tools