Antibody:	anti-neurofilament clone 2H3
Detection system:	Biotinylated secondary antibody/[Strept]avidin coupled to horseradish peroxidase

Structure	Level	Pattern	Image	Note
TS17: cranial nerve	Present		3A

Structure	Level	Pattern	Image	Note
TS17: cranial nerve	Present		3B	In this mutant nerves extend in the correct direction to their targets but defasciculate and occupy a wider area compared to wild-type embryos. No abnormality was seen in the oculomotor nerve.

Structure	Level	Pattern	Image	Note
TS17: cranial nerve	Present		3C

Structure	Level	Pattern	Image	Note
TS17: cranial nerve	Present		3D	In this mutant nerves extend in the correct direction to their targets but defasciculate and occupy a wider area compared to wild-type embryos. No abnormality was seen in the oculomotor nerve.

Structure	Level	Pattern	Image	Note
TS17: cranial nerve	Present		3E

Structure	Level	Pattern	Image	Note
TS17: cranial nerve	Present		3F	In this mutant the terminals of the vagus and accessory nerves spread out compared to wild-type embryos.

Structure	Level	Pattern	Image	Note
TS20: face	Present		4A	Expression is in the trigeminal nerve which innervates a region around the eye, extending into the maxillary process and muzzle.
TS20: trigeminal nerve	Present		4A	Expression is in the trigeminal nerve which innervates a region around the eye, extending into the maxillary process and muzzle.
TS20: cornea	Present	Regionally restricted	4A	Expression is in the trigeminal, sympathetic, and ciliary nerves at the periphery of the cornea.
TS20: nerve	Present	Regionally restricted	4A	Expression is in the trigeminal, sympathetic, and ciliary nerves at the periphery of the cornea.
TS20: lens primordium	Absent		4A

Structure	Level	Pattern	Image	Note
TS20: face	Present	Regionally restricted	4B	This mutant has abnormalities in the projection patterns, it extends further and occupied twice as much area as compared to wild-type embryos.
TS20: trigeminal nerve	Present	Regionally restricted	4B	This mutant has abnormalities in the projection patterns, it extends further and occupied twice as much area as compared to wild-type embryos.
TS20: cornea	Present	Regionally restricted	4B	This mutant has abnormalities in the projection patterns in the cornea extending over the lens.
TS20: lens primordium	Present	Regionally restricted	4B	This mutant has abnormalities in the projection patterns in the cornea extending over the lens.
TS20: nerve	Present	Regionally restricted	4B	This mutant has abnormalities in the projection patterns in the cornea extending over the lens.

Structure	Level	Pattern	Image	Note
TS21: face	Present	Regionally restricted	4C	Expression is in the trigeminal nerve which innervates a region around the eye, extending into the maxillary process and muzzle.
TS21: trigeminal nerve	Present	Regionally restricted	4C	Expression is in the trigeminal nerve which innervates a region around the eye, extending into the maxillary process and muzzle.

Structure	Level	Pattern	Image	Note
TS21: face	Present	Regionally restricted	4D	In this mutant expression in trigeminal terminals extended to certain areas in the ventrocaudal quadrant of the face.
TS21: trigeminal nerve	Present	Regionally restricted	4D	In this mutant expression in trigeminal terminals extended to certain areas in the ventrocaudal quadrant of the face.

Structure	Level	Pattern	Image	Note
TS20: eye	Present	Regionally restricted	4E	Expression is in nerve fibers that enter the tunica fibrosa bulbi.
TS20: nerve	Present	Regionally restricted	4E	Expression is in nerve fibers that enter the tunica fibrosa bulbi.

Structure	Level	Pattern	Image	Note
TS20: lens primordium	Present	Regionally restricted	4F	Expression is in nerve fibers that enter the lens.
TS20: nerve	Present	Regionally restricted	4F	Expression is in nerve fibers that enter the lens.

Structure	Level	Pattern	Image	Note
TS20: forelimb nerve	Present		4G

Structure	Level	Pattern	Image	Note
TS20: forelimb nerve	Present		4H	In this mutant there are more axon branches than in controls, and reach the base of the handplate.

Structure	Level	Pattern	Image	Note
TS21: forelimb nerve	Present		4J	In this mutant there are more axon branches than in controls, and reach the base of the handplate.

Structure	Level	Pattern	Image	Note
TS20: spinal nerve	Present		4K	Expression is in spinal nerve projection in the dorsal muscles and stop at the sclerotome boundary.

Structure	Level	Pattern	Image	Note
TS20: spinal nerve	Present		4L	In this mutant expression in spinal nerves did not stop at the sclerotome boundary and crossed the dorsal midline.

Structure	Level	Pattern	Image	Note
TS21: spinal nerve	Present		4M	Expression is in spinal nerve projection in the dorsal muscles and stop at the sclerotome boundary.

Structure	Level	Pattern	Image	Note
TS20: dorsal root ganglion	Present		5A
TS20: sensory ganglion	Present		5A

Structure	Level	Pattern	Image	Note
TS20: dorsal root ganglion	Present		5B	This mutant has abnormal axon projections.
TS20: sensory ganglion	Present		5B	This mutant has abnormal axon projections.

Structure	Level	Pattern	Image	Note
TS20: sympathetic ganglion	Present		5C

Structure	Level	Pattern	Image	Note
TS20: sympathetic ganglion	Present		5D	This mutant has a disrupted shape of sympathetic chains with cell bodies scattered in clumps over a broader medial-lateral area.