Lfng RNA in situ Gene Expression Assay

Gene Expression Data
Assay Details

Assay

Reference:	J:289914 Brown RM 2nd, et al., Notch-mediated lateral induction is necessary to maintain vestibular prosensory identity during inner ear development. Dev Biol. 2020 Jun 1;462(1):74-84
Assay type:	RNA in situ
MGI Accession ID:	MGI:6761817
Gene symbol:	Lfng
Gene name:	LFNG O-fucosylpeptide 3-beta-N-acetylglucosaminyltransferase

Probe:	Lfng probe25
Probe preparation:	Antisense labelled with digoxigenin RNA
Visualized with:	Alkaline phosphatase

Results

2B1-1 ©

Specimen 2B WT E9.5: embryonic day 9.5 (more

)

Structure	Level	Pattern	Image	Note
TS15: otocyst epithelium	Present	Regionally restricted	2B1-1	Expressed in the macular prosensory domain.

2B2-1 ©

Specimen 2B cKO E9.5: embryonic day 9.5; Rbpj^tm1.1Hon/Rbpj^tm1Hon, Tg(Pax2-cre)1Akg/0 (more

)
Note: Conditional mutant.

Structure	Level	Pattern	Image	Note
TS15: otocyst epithelium	Present	Regionally restricted	2B2-1	Expressed in the macular prosensory domain, similar as in the control.

2B1-2 ©

Specimen 2B WT E10.5 Anterior: embryonic day 10.5 (more

)

Structure	Level	Pattern	Image	Note
TS17: otocyst epithelium	Present	Regionally restricted	2B1-2	Expressed in the macular prosensory domain.

2B2-2 ©

Specimen 2B cKO E10.5 Anterior: embryonic day 10.5; Rbpj^tm1.1Hon/Rbpj^tm1Hon, Tg(Pax2-cre)1Akg/0 (more

)
Note: Conditional mutant.

Structure	Level	Pattern	Image	Note
TS17: otocyst epithelium	Present	Regionally restricted	2B2-2	Reduced expression in the macular prosensory domain, compared with the control.

2B1-3 ©

Specimen 2B WT E10.5 Middle: embryonic day 10.5 (more

)

Structure	Level	Pattern	Image	Note
TS17: otocyst epithelium	Present	Regionally restricted	2B1-3	Expressed in the macular and cochlear prosensory domains.

2B2-3 ©

Specimen 2B cKO E10.5 Middle: embryonic day 10.5; Rbpj^tm1.1Hon/Rbpj^tm1Hon, Tg(Pax2-cre)1Akg/0 (more

)
Note: Conditional mutant.

Structure	Level	Pattern	Image	Note
TS17: otocyst epithelium	Present	Regionally restricted	2B2-3	Expressed in cochlear prosensory domain. A marked reduction in the size and intensity of expression in macular prosensory domain.

2B1-4 ©

Specimen 2B WT E11.5 Anterior: embryonic day 11.5 (more

)

Structure	Level	Pattern	Image	Note
TS19: otocyst epithelium	Present	Regionally restricted	2B1-4	Expressed in the macular prosensory domain.

2B2-4 ©

Specimen 2B cKO E11.5 Anterior: embryonic day 11.5; Rbpj^tm1.1Hon/Rbpj^tm1Hon, Tg(Pax2-cre)1Akg/0 (more

)
Note: Conditional mutant.

Structure	Level	Pattern	Image	Note
TS19: otocyst epithelium	Ambiguous	Regionally restricted	2B2-4	No expression in the macular prosensory domain was seen.

2B1-5 ©

Specimen 2B WT E11.5 Middle: embryonic day 11.5 (more

)

Structure	Level	Pattern	Image	Note
TS19: otocyst epithelium	Present	Regionally restricted	2B1-5	Expressed in the macular and cochlear prosensory domains.

2B2-5 ©

Specimen 2B cKO E11.5 Middle: embryonic day 11.5; Rbpj^tm1.1Hon/Rbpj^tm1Hon, Tg(Pax2-cre)1Akg/0 (more

)
Note: Conditional mutant.

Structure	Level	Pattern	Image	Note
TS19: otocyst epithelium	Ambiguous	Regionally restricted	2B2-5	A marked reduction in the size and intensity of expression in macular prosensory domain was seen from the figure, although author stated no expression in the results.

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