About   Help   FAQ
Gene Expression Data
Assay Details
Assay
Reference: J:241009 Himmelstein DS, et al., SHH E176/E177-Zn2+ conformation is required for signaling at endogenous sites. Dev Biol. 2017 Apr 15;424(2):221-235
Assay type: RT-PCR
MGI Accession ID: MGI:6761576
Gene symbol: Shh
Gene name: sonic hedgehog
Results
Image: 5B ©

 Sample Information Bands Other Sample Information
Lane AgeStructure Size Not Specified (a) Size Not Specified (b) Amount Genetic BackgroundMutant Allele(s)Sex Note
Shh+/- E9.5 TS15: future brain Present Absent Not Specified; total RNA involves: 129S1/Sv * 129X1/SvJ Shhtm1Chg/Shh+Not Specified
Shh+/- + MluI E9.5 TS15: future brain Present Absent Not Specified; total RNA involves: 129S1/Sv * 129X1/SvJ Shhtm1Chg/Shh+Not Specified Digested with MluI.
E177A/+ E9.5 TS15: future brain Present Absent Not Specified; total RNA Not Specified Shhtm1.1Jhdk/Shh+Not Specified
E177A/+ + MluI E9.5 TS15: future brain Present Present Not Specified; total RNA Not Specified Shhtm1.1Jhdk/Shh+Not Specified Digested with MluI.
E177A/- E9.5 TS15: future brain Present Absent Not Specified; total RNA involves: 129S1/Sv * 129X1/SvJ Shhtm1.1Jhdk/Shhtm1ChgNot Specified
E177A/- + MluI E9.5 TS15: future brain Absent Present Not Specified; total RNA involves: 129S1/Sv * 129X1/SvJ Shhtm1.1Jhdk/Shhtm1ChgNot Specified Digested with MluI.
Notes:
(a) Wild-type band.
(b) Smaller E177A mutant band after digestion with MluI.
Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
Citing These Resources
Funding Information
Warranty Disclaimer, Privacy Notice, Licensing, & Copyright
Send questions and comments to User Support. 		last database update
03/18/2025
MGI 6.24 		The Jackson Laboratory