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Gene Expression Data
Assay Details
Assay
Reference: J:149478 Mao J, et al., Fgf-dependent Etv4/5 activity is required for posterior restriction of Sonic Hedgehog and promoting outgrowth of the vertebrate limb. Dev Cell. 2009 Apr;16(4):600-6
Assay type: RNA in situ
MGI Accession ID: MGI:6758797
Gene symbol: Shh
Gene name: sonic hedgehog
Probe: Shh probe199
Probe preparation: Antisense labelled with digoxigenin RNA
Results
Specimen 3A E9.5 R26-EtvEnR: embryonic day 9.5; Gt(ROSA)26Sortm5(Etv4/en,-GFP)Amc/Gt(ROSA)26Sor+ (more )
Structure Level Pattern Image Note
TS15: limb mesenchyme Present Regionally restricted 3A R26-EtvEnR E9.5 Expression is in the posterior mesenchyme of the zone of polarizing activity.
Specimen 3A E9.5 Prx1-Cre;R26-EtvEnR: embryonic day 9.5; Gt(ROSA)26Sortm5(Etv4/en,-GFP)Amc/Gt(ROSA)26Sor+, Tg(Prrx1-cre)1Cjt/0 (more )
Note: Conditional mutant.
Structure Level Pattern Image Note
TS15: limb mesenchyme Present Regionally restricted 3A Prx1-Cre;R26-EtvEnR E9.5 The expression in the posterior mesenchyme of the zone of polarizing activity has extended anteriorly in this mutant compared to controls.
Specimen 3A E10.5 R26-EtvEnR: embryonic day 10.5; Gt(ROSA)26Sortm5(Etv4/en,-GFP)Amc/Gt(ROSA)26Sor+ (more )
Structure Level Pattern Image Note
TS17: limb mesenchyme Present Regionally restricted 3A R26-EtvEnR E10.5 Expression is in the posterior mesenchyme of the zone of polarizing activity.
Specimen 3A E10.5 Prx1-Cre;R26-EtvEnR: embryonic day 10.5; Gt(ROSA)26Sortm5(Etv4/en,-GFP)Amc/Gt(ROSA)26Sor+, Tg(Prrx1-cre)1Cjt/0 (more )
Note: Conditional mutant.
Structure Level Pattern Image Note
TS17: limb mesenchyme Present Regionally restricted 3A Prx1-Cre;R26-EtvEnR E10.5 Expression has extended across the entire distal limb bud to the anterior border in this mutant compared to controls.
Specimen 3A E11.5 R26-EtvEnR: embryonic day 11.5; Gt(ROSA)26Sortm5(Etv4/en,-GFP)Amc/Gt(ROSA)26Sor+ (more )
Structure Level Pattern Image Note
TS19: limb mesenchyme Present Regionally restricted 3A R26-EtvEnR E11.5 Expression is in the posterior mesenchyme of the zone of polarizing activity.
Specimen 3A E11.5 Prx1-Cre;R26-EtvEnR: embryonic day 11.5; Gt(ROSA)26Sortm5(Etv4/en,-GFP)Amc/Gt(ROSA)26Sor+, Tg(Prrx1-cre)1Cjt/0 (more )
Note: Conditional mutant.
Structure Level Pattern Image Note
TS19: limb mesenchyme Present Regionally restricted 3A Prx1-Cre;R26-EtvEnR E11.5 Ectopic expression faded along the distal anterior-posterior extent of the autopod, whereas expression remained strong at both the posterior and anterior margins.
Specimen S7A R26-EtvEnR: embryonic day 10.5; Gt(ROSA)26Sortm5(Etv4/en,-GFP)Amc/Gt(ROSA)26Sor+ (more )
Structure Level Pattern Image Note
TS17: limb mesenchyme Present Regionally restricted S7A R26-EtvEnR Expression is in the posterior mesenchyme of the zone of polarizing activity.
Specimen S7A Shh-Cre;R26-EtvEnR: embryonic day 10.5; Gt(ROSA)26Sortm5(Etv4/en,-GFP)Amc/Gt(ROSA)26Sor+, Shhtm1Amc/Shhtm2Amc, Tg(Prrx1-cre)1Cjt/0 (more )
Note: Conditional mutant.
Structure Level Pattern Image Note
TS17: limb mesenchyme Present Regionally restricted S7A Shh-Cre;R26-EtvEnR Expression in this mutant is similar to controls.
Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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01/20/2026
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