Alx4 Immunohistochemistry Gene Expression Assay

Gene Expression Data
Assay Details

Assay

Reference:	J:111643 Boras-Granic K, et al., Genetic interaction between Lef1 and Alx4 is required for early embryonic development. Int J Dev Biol. 2006;50(7):601-10
Assay type:	Immunohistochemistry
MGI Accession ID:	MGI:6755165
Gene symbol:	Alx4
Gene name:	aristaless-like homeobox 4

Antibody:	anti-Alx4
Detection system:	Secondary antibody coupled to fluorescein

Results

Specimen 2A/C: embryonic day 9.0 (more

)
Note: Double labeled: green - Alx4; red - Lef1 (assay MGI:6755151).

Structure	Level	Pattern	Image	Note
TS14: future telencephalon	Strong	Regionally restricted	2A , 2C	Expressed in the mesenchyme surrounding the telencephalic vesicle.

Specimen 2D/F: embryonic day 9.0 (more

)
Note: Double labeled: green - Alx4; red - Lef1 (assay MGI:6755151).

Structure	Level	Pattern	Image	Note
TS14: migrating neural crest	Present	Regionally restricted	2D , 2F	Expressed in potential neural crest cell migratory tract which followed a path dorsal to the otic vesicle.

Specimen 2J/L: embryonic day 9.0 (more

)
Note: Double labeled: green - Alx4; red - Lef1 (assay MGI:6755151).

Structure	Level	Pattern	Image	Note
TS14: trunk mesenchyme	Present	Regionally restricted	2L , 2J	Expressed in the mesenchymal cells surrounding the umbilical vein.
TS14: somite	Absent		2L , 2J

Specimen 9: embryonic day 9.5 (more

)
Note: Double labeled: green - Alx4; red - Pecam1 (assay MGI:6755166).

Structure	Level	Pattern	Image	Note
TS15: umbilical cord mesenchyme	Present		9_lower right , 9_lower left , 9_upper left	Expressed in mesenchymal cells surrounding the umbilical vein. No expression in in the nuclei of endothelial cells.

Contributing Projects: Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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