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Gene Expression Data
Assay Details
Assay
Reference: J:111643 Boras-Granic K, et al., Genetic interaction between Lef1 and Alx4 is required for early embryonic development. Int J Dev Biol. 2006;50(7):601-10
Assay type: Immunohistochemistry
MGI Accession ID: MGI:6755151
Gene symbol: Lef1
Gene name: lymphoid enhancer binding factor 1
Antibody: anti-Lef1
Detection system: Secondary antibody coupled to Cy3
Results
Specimen 2B/C: embryonic day 9.0 (more )
Note: Double labeled: red - Lef1; green - Alx4 (assay MGI:6755165).
Structure Level Pattern Image Note
TS14: future telencephalon Present Regionally restricted 2C , 2B Expressed in the mesenchyme surrounding the telencephalic vesicle.
TS14: Rathke's pouch Present 2B , 2C
TS14: embryo ectoderm Present 2B , 2C
Specimen 2E/F: embryonic day 9.0 (more )
Note: Double labeled: red - Lef1; green - Alx4 (assay MGI:6755165).
Structure Level Pattern Image Note
TS14: migrating neural crest Present Regionally restricted 2F , 2E Co-expressed with Alx4 in potential neural crest cell migratory tract which followed a path dorsal to the otic vesicle. Also expressed in cells medial to the otic vesicle, between the otic vesicle and the neural tube.
TS14: neural tube Present 2F , 2E Expressed in the nuclei.
TS14: otic placode Present 2F , 2E Expressed in the nuclei.
Specimen 2K/L: embryonic day 9.0 (more )
Note: Double labeled: red - Lef1; green - Alx4 (assay MGI:6755165).
Structure Level Pattern Image Note
TS14: trunk mesenchyme Present Regionally restricted 2K , 2L Co-expressed with Alx4 in the mesenchymal cells surrounding the umbilical vein.
TS14: neural tube Present 2K , 2L Weaker expression.
Specimen 8: embryonic day 9.5 (more )
Note: Double labeled: red - Lef1; green - Pecam1 (assay MGI:6755150).
Structure Level Pattern Image Note
TS15: trunk mesenchyme Strong Regionally restricted 8F , 8E , 8A , 8D Expressed in the mesenchymal cells surrounding the dorsal aorta.
TS15: dorsal aorta Trace Regionally restricted 8A , 8E , 8F , 8D Very weak expression just above background levels was seen in the nuclei of endothelial cells.
Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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03/25/2025
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